基于反义寡核苷酸共轭金纳米结构的电化学生物传感器用于检测 SARS-CoV-2

IF 7.5 Q1 CHEMISTRY, PHYSICAL
Tejaswini P. Patil , Vishakha S. Parkhe , Somnath S. Kundale , Rajanish K. Kamat , Tukaram D. Dongale , Rajendra S Patil , Arpita P. Tiwari
{"title":"基于反义寡核苷酸共轭金纳米结构的电化学生物传感器用于检测 SARS-CoV-2","authors":"Tejaswini P. Patil ,&nbsp;Vishakha S. Parkhe ,&nbsp;Somnath S. Kundale ,&nbsp;Rajanish K. Kamat ,&nbsp;Tukaram D. Dongale ,&nbsp;Rajendra S Patil ,&nbsp;Arpita P. Tiwari","doi":"10.1016/j.apsadv.2024.100618","DOIUrl":null,"url":null,"abstract":"<div><p>Nanoconstructs of gold nanoparticles (AuNPs) conjugated with SARS-CoV-2 specific antisense oligonucleotides (ASO) have been utilized to develop sensitive electrochemical nucleic acid biosensor for the detection of SARS-CoV-2 RNA. AuNPs were prepared through a one-pot synthesis method by utilizing Poly-L-Lysine (PLL) biopolymer and as synthesised AuNP were characterized by various analytical techniques such as UV–Vis spectroscopy, X-ray Diffraction (XRD) analysis, Fourier Transform Infra-Red spectroscopy (FT-IR), zeta potential, and Transmission Electron Microscopy (TEM). Poly-L-Lysine functionalized AuNPs (PLL-AuNPs) nanoconstructs platform was employed for immobilization of SARS-CoV-2 specific antisense oligonucleotides (ASO-conjugated PLL-AuNPs) via electrostatic interactions. The PLL-AuNPs were drop casted on glassy carbon electrode (GCE) following immobilization of ASO for fabrication of electrochemical biosensor. The ASO-conjugated PLL-AuNPs nanoconstructs were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The responsiveness of ASO-conjugated PLL-AuNPs nanoconstructs in presence SARS-CoV-2 RNA was monitored using the DPV, SWV and EIS technique, where methylene blue was employed as an electrochemical indicator for DNA-RNA hybridization detection. The biosensor exhibits a detection range for SARS-CoV-2 RNA infection ranging from 0 to 100 nM, with a limit of detection at 30.2 nM. The electrode, modified with ASO-conjugated PLL-AuNPs, was employed for the detection of SARS-CoV-2 RNA from clinical samples collected from COVID-19-positive individuals.</p></div>","PeriodicalId":34303,"journal":{"name":"Applied Surface Science Advances","volume":null,"pages":null},"PeriodicalIF":7.5000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2666523924000461/pdfft?md5=227fac56f1013d066bea1266181a6daa&pid=1-s2.0-S2666523924000461-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Antisense oligonucleotide conjugated gold nanoconstructs-based electrochemical biosensor for detection of SARS-CoV-2\",\"authors\":\"Tejaswini P. Patil ,&nbsp;Vishakha S. Parkhe ,&nbsp;Somnath S. Kundale ,&nbsp;Rajanish K. Kamat ,&nbsp;Tukaram D. Dongale ,&nbsp;Rajendra S Patil ,&nbsp;Arpita P. Tiwari\",\"doi\":\"10.1016/j.apsadv.2024.100618\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Nanoconstructs of gold nanoparticles (AuNPs) conjugated with SARS-CoV-2 specific antisense oligonucleotides (ASO) have been utilized to develop sensitive electrochemical nucleic acid biosensor for the detection of SARS-CoV-2 RNA. AuNPs were prepared through a one-pot synthesis method by utilizing Poly-L-Lysine (PLL) biopolymer and as synthesised AuNP were characterized by various analytical techniques such as UV–Vis spectroscopy, X-ray Diffraction (XRD) analysis, Fourier Transform Infra-Red spectroscopy (FT-IR), zeta potential, and Transmission Electron Microscopy (TEM). Poly-L-Lysine functionalized AuNPs (PLL-AuNPs) nanoconstructs platform was employed for immobilization of SARS-CoV-2 specific antisense oligonucleotides (ASO-conjugated PLL-AuNPs) via electrostatic interactions. The PLL-AuNPs were drop casted on glassy carbon electrode (GCE) following immobilization of ASO for fabrication of electrochemical biosensor. The ASO-conjugated PLL-AuNPs nanoconstructs were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The responsiveness of ASO-conjugated PLL-AuNPs nanoconstructs in presence SARS-CoV-2 RNA was monitored using the DPV, SWV and EIS technique, where methylene blue was employed as an electrochemical indicator for DNA-RNA hybridization detection. The biosensor exhibits a detection range for SARS-CoV-2 RNA infection ranging from 0 to 100 nM, with a limit of detection at 30.2 nM. The electrode, modified with ASO-conjugated PLL-AuNPs, was employed for the detection of SARS-CoV-2 RNA from clinical samples collected from COVID-19-positive individuals.</p></div>\",\"PeriodicalId\":34303,\"journal\":{\"name\":\"Applied Surface Science Advances\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.5000,\"publicationDate\":\"2024-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2666523924000461/pdfft?md5=227fac56f1013d066bea1266181a6daa&pid=1-s2.0-S2666523924000461-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Surface Science Advances\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666523924000461\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, PHYSICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Surface Science Advances","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666523924000461","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0

摘要

利用与 SARS-CoV-2 特异性反义寡核苷酸(ASO)共轭的金纳米粒子(AuNPs)纳米结构开发了灵敏的电化学核酸生物传感器,用于检测 SARS-CoV-2 RNA。利用聚-L-赖氨酸(PLL)生物聚合物通过一锅合成法制备了 AuNPs,并通过紫外可见光谱、X 射线衍射(XRD)分析、傅立叶变换红外光谱(FT-IR)、ZETA 电位和透射电子显微镜(TEM)等各种分析技术对合成的 AuNPs 进行了表征。利用聚赖氨酸功能化 AuNPs(PLL-AuNPs)纳米结构平台,通过静电相互作用固定 SARS-CoV-2 特异性反义寡核苷酸(ASO 共轭 PLL-AuNPs)。在固定 ASO 后,将 PLL-AuNPs 滴铸在玻璃碳电极(GCE)上,用于制造电化学生物传感器。通过循环伏安法(CV)、差分脉冲伏安法(DPV)和电化学阻抗谱(EIS)技术对 ASO 共轭的 PLL-AuNPs 纳米结构进行了表征。利用 DPV、SWV 和 EIS 技术监测了 ASO 共轭 PLL-AuNPs 纳米结构在 SARS-CoV-2 RNA 存在下的响应性,其中亚甲基蓝被用作 DNA-RNA 杂交检测的电化学指示剂。该生物传感器对 SARS-CoV-2 RNA 感染的检测范围为 0 至 100 nM,检测限为 30.2 nM。该电极由 ASO 共轭 PLL-AuNPs 修饰,用于检测从 COVID-19 阳性个体采集的临床样本中的 SARS-CoV-2 RNA。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antisense oligonucleotide conjugated gold nanoconstructs-based electrochemical biosensor for detection of SARS-CoV-2

Antisense oligonucleotide conjugated gold nanoconstructs-based electrochemical biosensor for detection of SARS-CoV-2

Nanoconstructs of gold nanoparticles (AuNPs) conjugated with SARS-CoV-2 specific antisense oligonucleotides (ASO) have been utilized to develop sensitive electrochemical nucleic acid biosensor for the detection of SARS-CoV-2 RNA. AuNPs were prepared through a one-pot synthesis method by utilizing Poly-L-Lysine (PLL) biopolymer and as synthesised AuNP were characterized by various analytical techniques such as UV–Vis spectroscopy, X-ray Diffraction (XRD) analysis, Fourier Transform Infra-Red spectroscopy (FT-IR), zeta potential, and Transmission Electron Microscopy (TEM). Poly-L-Lysine functionalized AuNPs (PLL-AuNPs) nanoconstructs platform was employed for immobilization of SARS-CoV-2 specific antisense oligonucleotides (ASO-conjugated PLL-AuNPs) via electrostatic interactions. The PLL-AuNPs were drop casted on glassy carbon electrode (GCE) following immobilization of ASO for fabrication of electrochemical biosensor. The ASO-conjugated PLL-AuNPs nanoconstructs were characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) techniques. The responsiveness of ASO-conjugated PLL-AuNPs nanoconstructs in presence SARS-CoV-2 RNA was monitored using the DPV, SWV and EIS technique, where methylene blue was employed as an electrochemical indicator for DNA-RNA hybridization detection. The biosensor exhibits a detection range for SARS-CoV-2 RNA infection ranging from 0 to 100 nM, with a limit of detection at 30.2 nM. The electrode, modified with ASO-conjugated PLL-AuNPs, was employed for the detection of SARS-CoV-2 RNA from clinical samples collected from COVID-19-positive individuals.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
8.10
自引率
1.60%
发文量
128
审稿时长
66 days
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信