通过表达 OPG、RANK 和 RANKL,用纳米颗粒稻壳液体烟雾治疗牙周炎。

IF 3 Q3 MATERIALS SCIENCE, BIOMATERIALS
International Journal of Biomaterials Pub Date : 2024-06-14 eCollection Date: 2024-01-01 DOI:10.1155/2024/5015893
Ira Arundina, Theresia Indah Budhy, Aqsa Sjuhada Oki, Meircurius Dwi Condro Surboyo, Arvind Babu Rajendra Santosh, Sidarningsih, Indeswati Diyatri, Tytania Rahmaputry, Arya Pradana, Mohammad Iqbal, Azzahra Salsabila Adira Moelyanto
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引用次数: 0

摘要

导言:采用亚微米级纳米材料治疗牙周炎有望增强骨生成并促进牙周细胞增殖。本研究旨在通过评估骨保护素(OPG)、核因子-kβ受体激活剂(RANK)和核因子-kβ受体激活剂配体(RANKL)的表达,评估基于纳米颗粒的稻壳液体烟雾(n-RHLS)在牙周炎动物模型中的潜力:将 109 CFU/ml 的牙龈卟啉单胞菌接种到 28 只雄性 Wistar 大鼠的下颌切牙沟区域,造成牙周炎,然后用 n-RHLS 治疗,用生理盐水对照。治疗后 2 天和 7 天,对下颌切牙进行免疫组化染色,以评估 OPG、RANK 和 RANKL 的表达:结果:OPG 的表达在治疗 2 天和 7 天后都有明显增加,而 RANKL 的表达在使用 n-RHLS 治疗 7 天后明显下降(p < 0.05)。相比之下,RANK 的表达与对照组相比没有明显差异(p > 0.05):结论:稻壳纳米颗粒衍生的纳米结构液态烟雾(n-RHLS)具有治疗牙周炎的潜力,尤其是通过调节OPG/RANK/RANKL的表达来支持牙周组织的健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression.

Introduction: Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (n-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kβ (RANK), and receptor activator of nuclear factor-kβ ligand (RANKL).

Methods: Twenty-eight male Wistar rats were inoculated with 109 CFU/ml of Porphyromonas gingivalis in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment.

Results: OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (p < 0.05). In contrast, RANK expression did not show significant differences compared to the control groups (p > 0.05).

Conclusion: Nanostructured liquid smoke derived from rice husk nanoparticles (n-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health.

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来源期刊
International Journal of Biomaterials
International Journal of Biomaterials MATERIALS SCIENCE, BIOMATERIALS-
CiteScore
4.30
自引率
3.20%
发文量
50
审稿时长
21 weeks
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