{"title":"通过表达 OPG、RANK 和 RANKL,用纳米颗粒稻壳液体烟雾治疗牙周炎。","authors":"Ira Arundina, Theresia Indah Budhy, Aqsa Sjuhada Oki, Meircurius Dwi Condro Surboyo, Arvind Babu Rajendra Santosh, Sidarningsih, Indeswati Diyatri, Tytania Rahmaputry, Arya Pradana, Mohammad Iqbal, Azzahra Salsabila Adira Moelyanto","doi":"10.1155/2024/5015893","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (<i>n</i>-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-k<i>β</i> (RANK), and receptor activator of nuclear factor-k<i>β</i> ligand (RANKL).</p><p><strong>Methods: </strong>Twenty-eight male Wistar rats were inoculated with 10<sup>9</sup> CFU/ml of <i>Porphyromonas gingivalis</i> in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment.</p><p><strong>Results: </strong>OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (<i>p</i> < 0.05). In contrast, RANK expression did not show significant differences compared to the control groups (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>Nanostructured liquid smoke derived from rice husk nanoparticles (<i>n</i>-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health.</p>","PeriodicalId":13704,"journal":{"name":"International Journal of Biomaterials","volume":"2024 ","pages":"5015893"},"PeriodicalIF":3.0000,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192596/pdf/","citationCount":"0","resultStr":"{\"title\":\"Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression.\",\"authors\":\"Ira Arundina, Theresia Indah Budhy, Aqsa Sjuhada Oki, Meircurius Dwi Condro Surboyo, Arvind Babu Rajendra Santosh, Sidarningsih, Indeswati Diyatri, Tytania Rahmaputry, Arya Pradana, Mohammad Iqbal, Azzahra Salsabila Adira Moelyanto\",\"doi\":\"10.1155/2024/5015893\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (<i>n</i>-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-k<i>β</i> (RANK), and receptor activator of nuclear factor-k<i>β</i> ligand (RANKL).</p><p><strong>Methods: </strong>Twenty-eight male Wistar rats were inoculated with 10<sup>9</sup> CFU/ml of <i>Porphyromonas gingivalis</i> in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment.</p><p><strong>Results: </strong>OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (<i>p</i> < 0.05). In contrast, RANK expression did not show significant differences compared to the control groups (<i>p</i> > 0.05).</p><p><strong>Conclusion: </strong>Nanostructured liquid smoke derived from rice husk nanoparticles (<i>n</i>-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health.</p>\",\"PeriodicalId\":13704,\"journal\":{\"name\":\"International Journal of Biomaterials\",\"volume\":\"2024 \",\"pages\":\"5015893\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2024-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11192596/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biomaterials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/5015893\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biomaterials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/5015893","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Nanoparticle-Based Rice Husk Liquid Smoke as Periodontitis Therapy through OPG, RANK, and RANKL Expression.
Introduction: Periodontitis therapy employing nanomaterials with submicron sizes holds promise for enhancing osteogenesis and facilitating periodontal cell proliferation. This study aims to assess the potential of nanoparticle-based rice husk liquid smoke (n-RHLS) in an animal model of periodontitis by evaluating the expression of osteoprotegerin (OPG), receptor activator of nuclear factor-kβ (RANK), and receptor activator of nuclear factor-kβ ligand (RANKL).
Methods: Twenty-eight male Wistar rats were inoculated with 109 CFU/ml of Porphyromonas gingivalis in the sulcus mandibular incisor region to create periodontitis and subsequently treated with n-RHLS while the control with saline. Immunohistochemical staining was performed on the mandibular incisor to assess OPG, RANK, and RANKL expression 2 and 7 days after treatment.
Results: OPG expression exhibited a significant increase at both 2 and 7 days, while RANKL expression decreased notably after 7 days of treatment using n-RHLS (p < 0.05). In contrast, RANK expression did not show significant differences compared to the control groups (p > 0.05).
Conclusion: Nanostructured liquid smoke derived from rice husk nanoparticles (n-RHLS) demonstrates potential as a therapeutic agent for periodontitis, especially on OPG/RANK/RANKL expression, by modulating OPG and RANKL expression to support periodontal tissue health.