在 COVID-19 期间对尸体组织进行 RT-qPCR 调查。

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2024-06-01 Epub Date: 2024-06-21 DOI:10.32725/jab.2024.013
Zhanna Berdygulova, Elina Maltseva, Yuliya Perfilyeva, Anna Nizkorodova, Andrey Zhigailov, Dinara Naizabayeva, Yekaterina O Ostapchuk, Saltanat Kuatbekova, Zhaniya Dosmagambet, Moldir Kuatbek, Akerke Bissenbay, Alena Cherusheva, Akzhigit Mashzhan, Nurshat Abdolla, Sanzhar Ashimbekov, Gulnara Ismagulova, Andrey Dmitrovskiy, Seidigapbar Mamadaliyev, Yuriy Skiba
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引用次数: 0

摘要

2020 年,哈萨克斯坦有许多病例出现 COVID-19 的临床症状,但鼻咽和口咽拭子的 PCR 结果为阴性。经临床和 CT 扫描(计算机断层扫描)确诊。在确认 SARS-CoV-2 感染时,PCR 结果为阴性的问题依然存在,这表明在此类病例中需要通过支气管肺泡灌洗液来确诊。目前还缺乏有关已故患者 SARS-CoV-2 感染确诊的信息。在这项研究中,研究人员对 2020 年至 2022 年期间死亡的 8 名推测感染 SARS-CoV-2 的患者的各种组织材料(包括肺、支气管和气管)进行了检查。这些患者在医院采集的鼻/咽拭子的 PCR 检测结果均为阴性。本研究在比较实验室中最常用的 RNA 分离方法的基础上,提出了一种改进的 RNA 分离方法:QIAamp Viral RNA Mini Kit 和基于 TRIzol 的方法。这种改良的核酸提取方案可用于在有争议的病例中通过 RT-qPCR 在死亡患者的组织中确认 SARS-CoV-2 感染。用这种方法从在-80 °C下保存超过32个月的尸体组织中重新提取的SARS-CoV-2的RNA进行RT-qPCR检测,仍然显示出高阳性结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
RT-qPCR investigation of post-mortem tissues during COVID-19.

In 2020, there were numerous cases in Kazakhstan with clinical symptoms of COVID-19 but negative PCR results in nasopharyngeal and oropharyngeal swabs. The diagnosis was confirmed clinically and by CT scans (computed tomography). The problem with such negative PCR results for SARS-CoV-2 infection confirmation still exists and indicates the need to confirm the diagnosis in the bronchoalveolar lavage in such cases. There is also a lack of information about confirmation of SARS-CoV-2 infection in deceased patients. In this study, various tissue materials, including lungs, bronchi, and trachea, were examined from eight patients who died, presumably from SARS-CoV-2 infection, between 2020 and 2022. Naso/oropharyngeal swabs taken from these patients in hospitals tested PCR negative for SARS-CoV-2. This study presents a modified RNA isolation method based on a comparison of the most used methods for RNA isolation in laboratories: QIAamp Viral RNA Mini Kit and TRIzol-based method. This modified nucleic acid extraction protocol can be used to confirm SARS-CoV-2 infection by RT-qPCR in the tissues of deceased patients in disputed cases. RT-qPCR with RNA of SARS-CoV-2 re-extracted with such method from post-mortem tissues that were stored at -80 °C for more than 32 months still demonstrated high-yielding positive results.

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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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