将基因组学带入实地:为森林恢复寻找种子的综合方法

IF 2.7 3区 生物学 Q2 PLANT SCIENCES
Anoob Prakash, Thibaut Capblancq, Kathryn Shallows, David Saville, Deborah Landau, Chad Landress, Tal Jacobs, Stephen Keller
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引用次数: 0

摘要

前提 全球人为变化威胁着森林生态系统的健康和生产力。协助迁移和重新造林是帮助减轻这些影响的工具。然而,如何寻找种子以确保高建树率和未来的适应性仍然是个问题。 方法 利用外显子组捕获测序,我们展示了一种计算方法,从种子来源候选列表中找到最佳的 n 组,这些 n 组共同实现了高遗传多样性(GD)和最小遗传负荷(GL),同时还提高了数量性状的可进化性。这种由三部分组成的策略(多样性-遗传负荷-可进化性)的好处是,既能提高近期的建植成功率,又能增强进化潜力,以应对未来的压力。大自然保护协会和中阿巴拉契亚云杉恢复计划的成员在 255 英亩的土地上种植了 58,000 株树苗。对一部分幼苗的成活率和生长变化进行了监测。 结果表明,相对于 GL,集合修复与单一来源修复在幼苗生长方面的 GD 和定量遗传变异都有所增加。在各个种植地点都没有观察到单一的 "超级来源";相反,监测结果表明,汇集多个来源有助于实现更高的 GD:GL 值和可进化性。 讨论 我们的研究显示了将基因组学融入地方规模恢复的潜力,以及在学术研究人员和应用保护管理人员之间建立合作关系的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bringing genomics to the field: An integrative approach to seed sourcing for forest restoration

Bringing genomics to the field: An integrative approach to seed sourcing for forest restoration

Premise

Global anthropogenic change threatens the health and productivity of forest ecosystems. Assisted migration and reforestation are tools to help mitigate these impacts. However, questions remain about how to approach sourcing seeds to ensure high establishment and future adaptability.

Methods

Using exome-capture sequencing, we demonstrate a computational approach to finding the best n-sets from a candidate list of seed sources that collectively achieve high genetic diversity (GD) and minimal genetic load (GL), while also increasing evolvability in quantitative traits. The benefits of this three-part strategy (diversity-load-evolvability) are to increase near-term establishment success while also boosting evolutionary potential to respond to future stressors. Members of The Nature Conservancy and the Central Appalachian Spruce Restoration Initiative planted 58,000 seedlings across 255 acres. A subset of seedlings was monitored for establishment success and variation in growth.

Results

The results show gains in GD relative to GL and increases in quantitative genetic variation in seedling growth for pooled vs. single-source restoration. No single “super source” was observed across planting sites; rather, monitoring results demonstrate that pooling of multiple sources helps achieve higher GD:GL and evolvability.

Discussion

Our study shows the potential for integrating genomics into local-scale restoration and the importance of building partnerships between academic researchers and applied conservation managers.

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来源期刊
CiteScore
7.30
自引率
0.00%
发文量
50
审稿时长
12 weeks
期刊介绍: Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences. APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.
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