Shanhui Wang, Chu Yuan, Chengbin Xu, Ditian Li, Han Zhang, Jing Wang, Xin Wang, Yan Li, Dian Jiao, Siliang Yuan, Han Chen, Dongru Qiu
{"title":"Denitrificimonas halotolerans sp.","authors":"Shanhui Wang, Chu Yuan, Chengbin Xu, Ditian Li, Han Zhang, Jing Wang, Xin Wang, Yan Li, Dian Jiao, Siliang Yuan, Han Chen, Dongru Qiu","doi":"10.1007/s10482-024-01987-5","DOIUrl":null,"url":null,"abstract":"<div><p>A Gram-stain-negative, facultative anaerobe, rod-shaped strain JX-1<sup>T</sup> was isolated from UASB sludge treating landfill leachate in Wuhan, China. The isolate is capable of growing under conditions of pH 6.0–11.0 (optimum, pH 7.0–8.0), temperature 4–42 ℃ (optimum, 20–30 ℃), 0–8.0% (w/v) NaCl (optimum, 5.0%), and ammonia nitrogen concentration of 200–5000 mg/L (optimum, 500 mg/L) on LB plates. The microorganism can utilize malic acid, D-galactose, L-rhamnose, inosine, and L-glutamic acid as carbon sources, but does not reduce nitrates and nitrites. The major fatty acids are C<sub>18:1</sub>ω7c/C<sub>18:1</sub>ω6c, iso-C<sub>15:0</sub>, and anteiso-C<sub>15:0</sub>. The respiratory quinones are Q9 (91.92%) and Q8 (8.08%). Polar lipids include aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, and phospholipid. Compared with other strains, strain JX-1<sup>T</sup> and <i>Denitrificimonas caeni</i> HY-14<sup>T</sup> have the highest values in terms of 16S rRNA gene sequence similarity (96.79%), average nucleotide identity (ANI; 76.06%), and average amino acid identity (AAI; 78.89%). Its digital DNA-DNA hybridization (dDDH) result is 20.3%. The genome of strain JX-1<sup>T</sup>, with a size of 2.78 Mb and 46.12 mol% G + C content, lacks genes for denitrification and dissimilatory nitrate reduction to ammonium (DNRA), but contains genes for ectoine synthesis as a secondary metabolite. The results of this polyphasic study allow genotypic and phenotypic differentiation of the analysed strain from the closest related species and confirm that the strain represents a novel species within the genus <i>Denitrificimonas</i>, for which the name <i>Denitrificimonas halotolerans</i> sp. nov. is proposed with JX-1<sup>T</sup> (= MCCC 1K08958<sup>T</sup> = KCTC 8395<sup>T</sup>) as the type strain.</p></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Denitrificimonas halotolerans sp. nov., a novel species isolated from UASB sludge treating landfill leachate\",\"authors\":\"Shanhui Wang, Chu Yuan, Chengbin Xu, Ditian Li, Han Zhang, Jing Wang, Xin Wang, Yan Li, Dian Jiao, Siliang Yuan, Han Chen, Dongru Qiu\",\"doi\":\"10.1007/s10482-024-01987-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A Gram-stain-negative, facultative anaerobe, rod-shaped strain JX-1<sup>T</sup> was isolated from UASB sludge treating landfill leachate in Wuhan, China. The isolate is capable of growing under conditions of pH 6.0–11.0 (optimum, pH 7.0–8.0), temperature 4–42 ℃ (optimum, 20–30 ℃), 0–8.0% (w/v) NaCl (optimum, 5.0%), and ammonia nitrogen concentration of 200–5000 mg/L (optimum, 500 mg/L) on LB plates. The microorganism can utilize malic acid, D-galactose, L-rhamnose, inosine, and L-glutamic acid as carbon sources, but does not reduce nitrates and nitrites. The major fatty acids are C<sub>18:1</sub>ω7c/C<sub>18:1</sub>ω6c, iso-C<sub>15:0</sub>, and anteiso-C<sub>15:0</sub>. The respiratory quinones are Q9 (91.92%) and Q8 (8.08%). Polar lipids include aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, and phospholipid. Compared with other strains, strain JX-1<sup>T</sup> and <i>Denitrificimonas caeni</i> HY-14<sup>T</sup> have the highest values in terms of 16S rRNA gene sequence similarity (96.79%), average nucleotide identity (ANI; 76.06%), and average amino acid identity (AAI; 78.89%). Its digital DNA-DNA hybridization (dDDH) result is 20.3%. The genome of strain JX-1<sup>T</sup>, with a size of 2.78 Mb and 46.12 mol% G + C content, lacks genes for denitrification and dissimilatory nitrate reduction to ammonium (DNRA), but contains genes for ectoine synthesis as a secondary metabolite. The results of this polyphasic study allow genotypic and phenotypic differentiation of the analysed strain from the closest related species and confirm that the strain represents a novel species within the genus <i>Denitrificimonas</i>, for which the name <i>Denitrificimonas halotolerans</i> sp. nov. is proposed with JX-1<sup>T</sup> (= MCCC 1K08958<sup>T</sup> = KCTC 8395<sup>T</sup>) as the type strain.</p></div>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":\"117 1\",\"pages\":\"\"},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-06-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10482-024-01987-5\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10482-024-01987-5","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Denitrificimonas halotolerans sp. nov., a novel species isolated from UASB sludge treating landfill leachate
A Gram-stain-negative, facultative anaerobe, rod-shaped strain JX-1T was isolated from UASB sludge treating landfill leachate in Wuhan, China. The isolate is capable of growing under conditions of pH 6.0–11.0 (optimum, pH 7.0–8.0), temperature 4–42 ℃ (optimum, 20–30 ℃), 0–8.0% (w/v) NaCl (optimum, 5.0%), and ammonia nitrogen concentration of 200–5000 mg/L (optimum, 500 mg/L) on LB plates. The microorganism can utilize malic acid, D-galactose, L-rhamnose, inosine, and L-glutamic acid as carbon sources, but does not reduce nitrates and nitrites. The major fatty acids are C18:1ω7c/C18:1ω6c, iso-C15:0, and anteiso-C15:0. The respiratory quinones are Q9 (91.92%) and Q8 (8.08%). Polar lipids include aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, and phospholipid. Compared with other strains, strain JX-1T and Denitrificimonas caeni HY-14T have the highest values in terms of 16S rRNA gene sequence similarity (96.79%), average nucleotide identity (ANI; 76.06%), and average amino acid identity (AAI; 78.89%). Its digital DNA-DNA hybridization (dDDH) result is 20.3%. The genome of strain JX-1T, with a size of 2.78 Mb and 46.12 mol% G + C content, lacks genes for denitrification and dissimilatory nitrate reduction to ammonium (DNRA), but contains genes for ectoine synthesis as a secondary metabolite. The results of this polyphasic study allow genotypic and phenotypic differentiation of the analysed strain from the closest related species and confirm that the strain represents a novel species within the genus Denitrificimonas, for which the name Denitrificimonas halotolerans sp. nov. is proposed with JX-1T (= MCCC 1K08958T = KCTC 8395T) as the type strain.
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.