脑外伤后死亡者的硬脑膜和存活时间测定:一项初步研究。

IF 1.5 4区 医学 Q2 MEDICINE, LEGAL
Forensic Science, Medicine and Pathology Pub Date : 2025-03-01 Epub Date: 2024-06-22 DOI:10.1007/s12024-024-00834-3
Marcello Benevento, Antonio d'Amati, Simona Nicolì, Laura Ambrosi, Jacek Baj, Davide Ferorelli, Giuseppe Ingravallo, Biagio Solarino
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引用次数: 0

摘要

背景:创伤性脑损伤(TBI)是年轻人发病和死亡的主要原因之一,也是法医病理学家关注的问题。许多学者尝试用不同的方法估算创伤性脑损伤后的存活时间(ST):本研究旨在提出一种创新的工作流程,通过观察硬脑膜(DM)的炎症反应来估算创伤后的存活时间:作者收集了 36 具尸体(20 具有创伤性脑损伤,16 具无创伤性脑损伤病史或体征)的硬脑膜样本。每个样本都用三种不同的一抗(CD15、CD68 和 CD3)进行免疫组化标记,共制作了 108 张切片。切片采用 QuPath 软件进行数字化和分析:结果:DM 参与了创伤性脑损伤后的炎症反应。CD15 免疫反应使我们能够区分创伤性脑损伤后立即死亡的受试者和创伤性脑损伤后数分钟或数小时死亡的受试者。CD3 免疫反应可用于区分死亡时间为数天和其他时间的受试者。此外,即使样本有腐败迹象,DM 样本的诊断率也是可以接受的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dura mater and survival time determination in individuals who died after traumatic brain injury: a preliminary study.

Background: Traumatic brain injury (TBI) is one of the major causes of morbidity and mortality among young people and is a matter of concern for forensic pathologists. Many authors have tried to estimate a person's survival time (ST) after TBI using different approaches.

Objective: The present study aimed to present an innovative workflow to estimate the ST after TBI by observing the inflammatory reaction of the dura mater (DM).

Methods: The authors collected DM samples from 36 cadavers (20 with TBI and 16 with no history or signs of TBI). Each sample was labelled via immunohistochemistry with three different primary antibodies, CD15, CD68, and CD3, yielding 108 slides in total. The slides were digitalized and analysed using QuPath software.

Results: The DM is involved in the inflammatory response after TBI. CD15 immunoreactivity allowed us to distinguish between subjects who died immediately after TBI and those with an ST of minutes or hours. CD3 immunoreactivity can be used to differentiate subjects with an ST of days from those with other STs. Moreover, the DM samples showed an acceptable diagnostic yield even in samples with signs of putrefaction.

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来源期刊
Forensic Science, Medicine and Pathology
Forensic Science, Medicine and Pathology MEDICINE, LEGAL-PATHOLOGY
CiteScore
3.90
自引率
5.60%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Forensic Science, Medicine and Pathology encompasses all aspects of modern day forensics, equally applying to children or adults, either living or the deceased. This includes forensic science, medicine, nursing, and pathology, as well as toxicology, human identification, mass disasters/mass war graves, profiling, imaging, policing, wound assessment, sexual assault, anthropology, archeology, forensic search, entomology, botany, biology, veterinary pathology, and DNA. Forensic Science, Medicine, and Pathology presents a balance of forensic research and reviews from around the world to reflect modern advances through peer-reviewed papers, short communications, meeting proceedings and case reports.
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