用油漆辊从水下表面采集海洋 eDNA 样本

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY
Simon Jarman , Jason B. Alexander , Kathryn L. Dawkins , Sherralee S. Lukehurst , Georgia M. Nester , Shaun Wilkinson , Michael J. Marnane , Justin I. McDonald , Travis S. Elsdon , Euan S. Harvey
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引用次数: 0

摘要

对海洋环境中存在的物种进行环境 DNA(eDNA)分析是目前最有效的生物多样性测量工具。确定和开发尽可能快速、经济、通用和实用的 eDNA 采样方法将提高海洋物种的检测率。eDNA 生物多样性调查的最佳结果来自于采用高水平重复采样的研究,因此任何能使采样更快、更便宜的方法都将提高科学成果。在这项研究中,我们比较了从水下海洋表面采集 eDNA 样本的几种方法的多样性检测:聚氨酯泡沫、尼龙棉签、超细纤维油漆滚筒和沉积物勺。所有方法都能鉴定出不同的物种,研究地点的 eDNA 代表了 250 个多细胞物种。我们发现,对于从水下海洋表面采集 eDNA 样本,广泛使用的小型油漆辊是一种有效、易于获得且经济实惠的方法。这种方法可以在人工采样不可行的情况下,使用加长杆或遥控潜水器对海洋 eDNA 进行采样。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Marine eDNA sampling from submerged surfaces with paint rollers

Environmental DNA (eDNA) analyses of species present in marine environments is the most effective biological diversity measurement tool currently available. eDNA sampling methods are an intrinsically important part of the eDNA biodiversity analysis process. Identification and development of eDNA sampling methods that are as rapid, affordable, versatile and practical as possible will improve rates of detection of marine species. Optimal outcomes of eDNA biodiversity surveys come from studies employing high levels of sampling replication, so any methods that make sampling faster and cheaper will improve scientific outcomes. eDNA sampling methods that can be applied more widely will also enable sampling from a greater range of marine surface micro-habitats, resulting in detection of a wider range of organisms. In this study, we compared diversity detection by several methods for sampling eDNA from submerged marine surfaces: polyurethane foam, nylon swabs, microfibre paint rollers, and sediment scoops. All of the methods produced a diverse range of species identifications, with >250 multicellular species represented by eDNA at the study site. We found that widely-available small paint rollers were an effective, readily available and affordable method for sampling eDNA from underwater marine surfaces. This approach enables the sampling of marine eDNA using extended poles, or potentially by remotely operated vehicles, where surface sampling by hand is impractical.

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来源期刊
Marine genomics
Marine genomics 生物-遗传学
CiteScore
3.60
自引率
5.30%
发文量
50
审稿时长
29 days
期刊介绍: The journal publishes papers on all functional and evolutionary aspects of genes, chromatin, chromosomes and (meta)genomes of marine (and freshwater) organisms. It deals with new genome-enabled insights into the broader framework of environmental science. Topics within the scope of this journal include: • Population genomics and ecology • Evolutionary and developmental genomics • Comparative genomics • Metagenomics • Environmental genomics • Systems biology More specific topics include: geographic and phylogenomic characterization of aquatic organisms, metabolic capacities and pathways of organisms and communities, biogeochemical cycles, genomics and integrative approaches applied to microbial ecology including (meta)transcriptomics and (meta)proteomics, tracking of infectious diseases, environmental stress, global climate change and ecosystem modelling.
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