miR-665 靶向 GNB3 在铝诱导神经细胞凋亡中的作用机制

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Chanting He , Jingjing Jia , Yang Lei , Qian Hu , Yulu Xin , Yafen Chu , Congying Liu , Qiao Niu
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引用次数: 0

摘要

方法将40只SD大鼠分为0、10、20和40 mM麦芽酚铝[Al(mal)3]组。细胞实验分为 0、100、200 和 400 μM Al(mal)3 剂量组和对照组、Al(mal)3 组、Al(mal)3+抑制剂 NC 组、Al(mal)3+miR-665 抑制剂干预组。水迷宫检测大鼠的学习记忆功能,HE染色观察大鼠海马CA1区神经元的形态和数量,流式细胞术检测PC12细胞的凋亡,PCR和Western印迹检测Caspase3、miR-665和GNB3/PI3K/AKT蛋白的表达。结果体内实验结果表明,随着 Al(mal)3 浓度的增加,大鼠的逃逸潜伏期延长,靶象限停留时间缩短,穿越平台的数量减少。此外,神经元排列松散,数量减少;Caspase3和miR-665表达增加,GNB3/PI3K/AKT蛋白表达减少。在体外实验中,随着Al(mal)3浓度的增加,PC12细胞的凋亡率增加,Caspase3、miR-665和GNB3/PI3K/AKT蛋白的表达与大鼠结果一致。结论 miR-665通过靶向GNB3和调控PI3K/AKT通路在铝诱导的神经元凋亡中发挥重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The mechanism of miR-665 targeting GNB3 in aluminum-induced neuronal apoptosis

Background

Aluminum exerts neurotoxic effects through various mechanisms, mainly manifested as impaired learning and memory function.

Methods

Forty SD rats were divided into 0, 10, 20, and 40 mM maltol aluminum [Al(mal)3] groups. Cell experiments are divided into 0, 100, 200, and 400 μM Al(mal)3 dose group and control, Al(mal)3, Al(mal)3+inhibitor NC, Al(mal)3+miR-665 inhibitor intervention group. Water maze was used to detect the learning and memory function of rats, HE staining was used to observe the morphology and number of neurons in the CA1 area of the rat hippocampus, Flow cytometry was used to detect the apoptosis of PC12 cells, PCR and Western blotting were used to detect the expression of Caspase3, miR-665 and GNB3/PI3K/AKT proteins. The target binding relationship between miR-665 and GNB3 was verified by double luciferase reporter gene experiment.

Results

In vivo experimental results showed that with the increase of Al(mal)3 concentration, the escape latency of rats was prolonged, the target quadrant dwell time was shortened, and the number of crossing platform was reduced. Moreover, the arrangement of neurons was loose and the number decreased; the expression of Caspase3 and miR-665 increased, while the expression of GNB3/PI3K/AKT proteins decreased. In vitro experiments, with the increase of Al(mal)3 concentration, apoptosis rate of PC12 cells increased, the expression of Caspase3, miR-665 and GNB3/PI3K/AKT proteins were consistent with rat results. After inhibiting miR-665 in the intervention group experiment, apoptosis rate of PC12 cells in the aluminum exposure group decreased, the expression of Caspase3 and miR-665 decreased, and the expression of GNB3/PI3K/AKT proteins increased.

Conclusion

MiR-665 plays an important role in aluminum induced neuronal apoptosis by targeting GNB3 and regulating the PI3K/AKT pathway.

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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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