{"title":"全蛋白质组分析揭示了水稻(Oryza sativa)中的 G 蛋白偶联受体样蛋白。","authors":"Dinesh K Yadav, Gyan Prakash Srivastava, Ananya Singh, Madhavi Singh, Neelam Yadav, Narendra Tuteja","doi":"10.1080/15592324.2024.2365572","DOIUrl":null,"url":null,"abstract":"<p><p>G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane proteins in metazoans that mediate the regulation of various physiological responses to discrete ligands through heterotrimeric G protein subunits. The existence of GPCRs in plant is contentious, but their comparable crucial role in various signaling pathways necessitates the identification of novel remote GPCR-like proteins that essentially interact with the plant G protein α subunit and facilitate the transduction of various stimuli. In this study, we identified three putative GPCR-like proteins (OsGPCRLPs) (LOC_Os06g09930.1, LOC_Os04g36630.1, and LOC_Os01g54784.1) in the rice proteome using a stringent bioinformatics workflow. The identified OsGPCRLPs exhibited a canonical GPCR 'type I' 7TM topology, patterns, and biologically significant sites for membrane anchorage and desensitization. Cluster-based interactome mapping revealed that the identified proteins interact with the G protein α subunit which is a characteristic feature of GPCRs. Computational results showing the interaction of identified GPCR-like proteins with G protein α subunit and its further validation by the membrane yeast-two-hybrid assay strongly suggest the presence of GPCR-like 7TM proteins in the rice proteome. The absence of a regulator of G protein signaling (RGS) box in the C- terminal domain, and the presence of signature motifs of canonical GPCR in the identified OsGPCRLPs strongly suggest that the rice proteome contains GPCR-like proteins that might be involved in signal transduction.</p>","PeriodicalId":94172,"journal":{"name":"Plant signaling & behavior","volume":"19 1","pages":"2365572"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195488/pdf/","citationCount":"0","resultStr":"{\"title\":\"Proteome-wide analysis reveals G protein-coupled receptor-like proteins in rice (<i>Oryza sativa</i>).\",\"authors\":\"Dinesh K Yadav, Gyan Prakash Srivastava, Ananya Singh, Madhavi Singh, Neelam Yadav, Narendra Tuteja\",\"doi\":\"10.1080/15592324.2024.2365572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane proteins in metazoans that mediate the regulation of various physiological responses to discrete ligands through heterotrimeric G protein subunits. The existence of GPCRs in plant is contentious, but their comparable crucial role in various signaling pathways necessitates the identification of novel remote GPCR-like proteins that essentially interact with the plant G protein α subunit and facilitate the transduction of various stimuli. In this study, we identified three putative GPCR-like proteins (OsGPCRLPs) (LOC_Os06g09930.1, LOC_Os04g36630.1, and LOC_Os01g54784.1) in the rice proteome using a stringent bioinformatics workflow. The identified OsGPCRLPs exhibited a canonical GPCR 'type I' 7TM topology, patterns, and biologically significant sites for membrane anchorage and desensitization. Cluster-based interactome mapping revealed that the identified proteins interact with the G protein α subunit which is a characteristic feature of GPCRs. Computational results showing the interaction of identified GPCR-like proteins with G protein α subunit and its further validation by the membrane yeast-two-hybrid assay strongly suggest the presence of GPCR-like 7TM proteins in the rice proteome. The absence of a regulator of G protein signaling (RGS) box in the C- terminal domain, and the presence of signature motifs of canonical GPCR in the identified OsGPCRLPs strongly suggest that the rice proteome contains GPCR-like proteins that might be involved in signal transduction.</p>\",\"PeriodicalId\":94172,\"journal\":{\"name\":\"Plant signaling & behavior\",\"volume\":\"19 1\",\"pages\":\"2365572\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11195488/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant signaling & behavior\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/15592324.2024.2365572\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/6/21 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant signaling & behavior","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/15592324.2024.2365572","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/6/21 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
G 蛋白偶联受体(GPCR)是元类动物中最大的跨膜蛋白家族,通过异三聚体 G 蛋白亚基介导对离散配体的各种生理反应的调节。植物中是否存在 GPCR 尚存争议,但由于它们在各种信号通路中发挥着相当关键的作用,因此有必要鉴定新型的远端 GPCR 类蛋白,这些蛋白本质上与植物 G 蛋白 α 亚基相互作用,促进各种刺激的转导。在本研究中,我们采用严格的生物信息学工作流程,在水稻蛋白质组中鉴定出了三个假定的类 GPCR 蛋白(OsGPCRLPs)(LOC_Os06g09930.1、LOC_Os04g36630.1 和 LOC_Os01g54784.1)。鉴定出的 OsGPCRLPs 具有典型的 GPCR "I 型 "7TM 拓扑结构、模式以及具有生物学意义的膜锚定和脱敏位点。基于聚类的相互作用组图谱显示,鉴定出的蛋白质与 G 蛋白 α 亚基相互作用,这是 GPCR 的一个特征。计算结果显示了已鉴定的类 GPCR 蛋白与 G 蛋白 α 亚基的相互作用,并通过膜酵母-双杂交试验进一步验证了这一点,这有力地表明了水稻蛋白质组中存在类 GPCR 7TM 蛋白。在已鉴定的 OsGPCRLPs 的 C 端结构域中没有 G 蛋白信号转导调节器(RGS)框,而且存在典型 GPCR 的标志性基序,这些都有力地表明水稻蛋白质组中含有可能参与信号转导的 GPCR 样蛋白。
Proteome-wide analysis reveals G protein-coupled receptor-like proteins in rice (Oryza sativa).
G protein-coupled receptors (GPCRs) constitute the largest family of transmembrane proteins in metazoans that mediate the regulation of various physiological responses to discrete ligands through heterotrimeric G protein subunits. The existence of GPCRs in plant is contentious, but their comparable crucial role in various signaling pathways necessitates the identification of novel remote GPCR-like proteins that essentially interact with the plant G protein α subunit and facilitate the transduction of various stimuli. In this study, we identified three putative GPCR-like proteins (OsGPCRLPs) (LOC_Os06g09930.1, LOC_Os04g36630.1, and LOC_Os01g54784.1) in the rice proteome using a stringent bioinformatics workflow. The identified OsGPCRLPs exhibited a canonical GPCR 'type I' 7TM topology, patterns, and biologically significant sites for membrane anchorage and desensitization. Cluster-based interactome mapping revealed that the identified proteins interact with the G protein α subunit which is a characteristic feature of GPCRs. Computational results showing the interaction of identified GPCR-like proteins with G protein α subunit and its further validation by the membrane yeast-two-hybrid assay strongly suggest the presence of GPCR-like 7TM proteins in the rice proteome. The absence of a regulator of G protein signaling (RGS) box in the C- terminal domain, and the presence of signature motifs of canonical GPCR in the identified OsGPCRLPs strongly suggest that the rice proteome contains GPCR-like proteins that might be involved in signal transduction.