Jonas Wüster, Norbert Neckel, Florian Sterzik, Li Xiang-Tischhauser, Dirk Barnewitz, Antje Genzel, Steffen Koerdt, Carsten Rendenbach, Christian Müller-Mai, Max Heiland, Susanne Nahles, Christine Knabe
{"title":"基于羟基磷灰石的合成骨移植材料与现有骨替代材料相比对雌性肩胛骨临界大小骨缺损再生的影响。","authors":"Jonas Wüster, Norbert Neckel, Florian Sterzik, Li Xiang-Tischhauser, Dirk Barnewitz, Antje Genzel, Steffen Koerdt, Carsten Rendenbach, Christian Müller-Mai, Max Heiland, Susanne Nahles, Christine Knabe","doi":"10.1093/rb/rbae041","DOIUrl":null,"url":null,"abstract":"<p><p>Lately, the potential risk of disease transmission due to the use of bovine-derived bone substitutes has become obvious, demonstrating the urgent need for a synthetic grafting material with comparable bioactive behaviour and properties. Therefore, the effect of a synthetic hydroxyapatite (HA) (Osbone<sup>®</sup>) bone grafting material on bone regeneration was evaluated 2 weeks, 1 month, and 3, 6, 12 and 18 months after implantation in critical-size bone defects in the ovine scapula and compared to that of a bovine-derived HA (Bio-Oss<sup>®</sup>) and β-tricalcium phosphate (TCP) (Cerasorb<sup>®</sup> M). New bone formation and the biodegradability of the bone substitutes were assessed histomorphometrically. Hard tissue histology and immunohistochemical analysis were employed to characterize collagen type I, alkaline phosphatase, osteocalcin, as well as bone sialoprotein expression in the various cell and matrix components of the bone tissue to evaluate the bioactive properties of the bone grafting materials. No inflammatory tissue response was detected with any of the bone substitute materials studied. After 3 and 6 months, β-TCP (Cerasorb<sup>®</sup> M) showed superior bone formation when compared to both HA-based materials (3 months: β-TCP 55.65 ± 2.03% vs. SHA 49.05 ± 3.84% and BHA 47.59 ± 1.97%; <i>p </i>≤<i> </i>0.03; 6 months: β-TCP 62.03 ± 1.58%; SHA: 55.83 ± 2.59%; BHA: 53.44 ± 0.78%; <i>p </i>≤<i> </i>0.04). Further, after 12 and 18 months, a similar degree of bone formation and bone-particle contact was noted for all three bone substitute materials without any significant differences. The synthetic HA supported new bone formation, osteogenic marker expression, matrix mineralization and good bone-bonding behaviour to an equal and even slightly superior degree compared to the bovine-derived HA. As a result, synthetic HA can be regarded as a valuable alternative to the bovine-derived HA without the potential risk of disease transmission.</p>","PeriodicalId":20929,"journal":{"name":"Regenerative Biomaterials","volume":null,"pages":null},"PeriodicalIF":5.6000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11187503/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of a synthetic hydroxyapatite-based bone grafting material compared to established bone substitute materials on regeneration of critical-size bone defects in the ovine scapula.\",\"authors\":\"Jonas Wüster, Norbert Neckel, Florian Sterzik, Li Xiang-Tischhauser, Dirk Barnewitz, Antje Genzel, Steffen Koerdt, Carsten Rendenbach, Christian Müller-Mai, Max Heiland, Susanne Nahles, Christine Knabe\",\"doi\":\"10.1093/rb/rbae041\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Lately, the potential risk of disease transmission due to the use of bovine-derived bone substitutes has become obvious, demonstrating the urgent need for a synthetic grafting material with comparable bioactive behaviour and properties. Therefore, the effect of a synthetic hydroxyapatite (HA) (Osbone<sup>®</sup>) bone grafting material on bone regeneration was evaluated 2 weeks, 1 month, and 3, 6, 12 and 18 months after implantation in critical-size bone defects in the ovine scapula and compared to that of a bovine-derived HA (Bio-Oss<sup>®</sup>) and β-tricalcium phosphate (TCP) (Cerasorb<sup>®</sup> M). New bone formation and the biodegradability of the bone substitutes were assessed histomorphometrically. Hard tissue histology and immunohistochemical analysis were employed to characterize collagen type I, alkaline phosphatase, osteocalcin, as well as bone sialoprotein expression in the various cell and matrix components of the bone tissue to evaluate the bioactive properties of the bone grafting materials. No inflammatory tissue response was detected with any of the bone substitute materials studied. After 3 and 6 months, β-TCP (Cerasorb<sup>®</sup> M) showed superior bone formation when compared to both HA-based materials (3 months: β-TCP 55.65 ± 2.03% vs. SHA 49.05 ± 3.84% and BHA 47.59 ± 1.97%; <i>p </i>≤<i> </i>0.03; 6 months: β-TCP 62.03 ± 1.58%; SHA: 55.83 ± 2.59%; BHA: 53.44 ± 0.78%; <i>p </i>≤<i> </i>0.04). Further, after 12 and 18 months, a similar degree of bone formation and bone-particle contact was noted for all three bone substitute materials without any significant differences. The synthetic HA supported new bone formation, osteogenic marker expression, matrix mineralization and good bone-bonding behaviour to an equal and even slightly superior degree compared to the bovine-derived HA. 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引用次数: 0
摘要
近来,使用牛源性骨替代物所带来的潜在疾病传播风险已变得十分明显,这表明迫切需要一种具有可比生物活性行为和特性的合成植骨材料。因此,我们评估了合成羟基磷灰石 (HA) (Osbone®) 骨移植材料在雌性肩胛骨临界大小骨缺损中植入 2 周、1 个月、3、6、12 和 18 个月后对骨再生的影响,并与牛源性 HA (Bio-Oss®) 和 β-磷酸三钙 (TCP) (Cerasorb® M) 进行了比较。新骨形成和骨替代物的生物降解性通过组织形态计量学进行评估。采用硬组织组织学和免疫组化分析来确定骨组织各种细胞和基质成分中 I 型胶原蛋白、碱性磷酸酶、骨钙素以及骨硅蛋白的表达,以评估骨移植材料的生物活性特性。所研究的骨替代材料均未发现炎症组织反应。3 个月和 6 个月后,β-TCP(Cerasorb® M)的骨形成优于两种基于 HA 的材料(3 个月:β-TCP 55.65 ± 2.03% vs. SHA 49.05 ± 3.84% 和 BHA 47.59 ± 1.97%;p ≤ 0.03;6 个月:β-TCP 62.03 ± 1.58%;SHA:55.83 ± 2.59%;BHA:53.44 ± 0.78%;p ≤ 0.04)。此外,12 个月和 18 个月后,三种骨替代材料的骨形成和骨颗粒接触程度相似,没有明显差异。与牛源性 HA 相比,合成 HA 对新骨形成、成骨标志物表达、基质矿化和良好的骨结合性能的支持程度相当,甚至略胜一筹。因此,合成 HA 可被视为牛源性 HA 的一种有价值的替代品,而且没有潜在的疾病传播风险。
Effect of a synthetic hydroxyapatite-based bone grafting material compared to established bone substitute materials on regeneration of critical-size bone defects in the ovine scapula.
Lately, the potential risk of disease transmission due to the use of bovine-derived bone substitutes has become obvious, demonstrating the urgent need for a synthetic grafting material with comparable bioactive behaviour and properties. Therefore, the effect of a synthetic hydroxyapatite (HA) (Osbone®) bone grafting material on bone regeneration was evaluated 2 weeks, 1 month, and 3, 6, 12 and 18 months after implantation in critical-size bone defects in the ovine scapula and compared to that of a bovine-derived HA (Bio-Oss®) and β-tricalcium phosphate (TCP) (Cerasorb® M). New bone formation and the biodegradability of the bone substitutes were assessed histomorphometrically. Hard tissue histology and immunohistochemical analysis were employed to characterize collagen type I, alkaline phosphatase, osteocalcin, as well as bone sialoprotein expression in the various cell and matrix components of the bone tissue to evaluate the bioactive properties of the bone grafting materials. No inflammatory tissue response was detected with any of the bone substitute materials studied. After 3 and 6 months, β-TCP (Cerasorb® M) showed superior bone formation when compared to both HA-based materials (3 months: β-TCP 55.65 ± 2.03% vs. SHA 49.05 ± 3.84% and BHA 47.59 ± 1.97%; p ≤0.03; 6 months: β-TCP 62.03 ± 1.58%; SHA: 55.83 ± 2.59%; BHA: 53.44 ± 0.78%; p ≤0.04). Further, after 12 and 18 months, a similar degree of bone formation and bone-particle contact was noted for all three bone substitute materials without any significant differences. The synthetic HA supported new bone formation, osteogenic marker expression, matrix mineralization and good bone-bonding behaviour to an equal and even slightly superior degree compared to the bovine-derived HA. As a result, synthetic HA can be regarded as a valuable alternative to the bovine-derived HA without the potential risk of disease transmission.
期刊介绍:
Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.