Zhaoqun Liu , Weilin Wang , Yanan Zong , Meijia Li , Yuqian Gao , Xiaoyu Xin , Ting Zhu , Lingling Wang , Linsheng Song
{"title":"去甲肾上腺素通过 A1AR-p38 MAPK-Relish 通路调节巨牡蛎粒细胞中 TNF 的表达。","authors":"Zhaoqun Liu , Weilin Wang , Yanan Zong , Meijia Li , Yuqian Gao , Xiaoyu Xin , Ting Zhu , Lingling Wang , Linsheng Song","doi":"10.1016/j.dci.2024.105217","DOIUrl":null,"url":null,"abstract":"<div><p>Norepinephrine (NE) is involved in regulating cytokine expression and phagocytosis of immune cells in the innate immunity of vertebrates. In the present study, the modulation mechanism of NE on the biosynthesis of TNFs in oyster granulocytes was explored. The transcripts of <em>Cg</em>TNF-1, <em>Cg</em>TNF-2 and <em>Cg</em>TNF-3 were highly expressed in granulocytes, and they were significantly up-regulated after LPS stimulation, while down-regulated after NE treatment. The phagocytic rate and apoptosis index of oyster granulocytes were also triggered by LPS stimulation and suppressed by NE treatment. The mRNA expressions of <em>Cg</em>MAPK14 and <em>Cg</em>Relish were significantly induced after NE treatment, and the translocation of <em>Cg</em>Relish from cytoplasm to nucleus was observed. The concentration of intracellular Ca<sup>2+</sup> in granulocytes was significantly up-regulated upon NE incubation, and this trend reverted after the treatment with DOX (specific antagonist for NE receptor, <em>Cg</em>A1AR-1). No obvious significance was observed in intracellular cAMP concentrations in the PBS, NE and NE + DOX groups. Once <em>Cg</em>A1AR-1 was blocked by DOX, the mRNA expressions of <em>Cg</em>MAPK14 and <em>Cg</em>Relish were significantly inhibited, and the translocation of <em>Cg</em>Relish from cytoplasm to nucleus was also dramatically suppressed, while the mRNA expression of <em>Cg</em>TNF-1 and the apoptosis index increased significantly to the same level with those in LPS group, respectively. These results collectively suggested that NE modulated TNF expression in oyster granulocyte through A1AR-p38 MAPK-Relish signaling pathway.</p></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Norepinephrine regulates TNF expression via the A1AR-p38 MAPK-Relish pathway in granulocytes of oyster Crassostrea gigas\",\"authors\":\"Zhaoqun Liu , Weilin Wang , Yanan Zong , Meijia Li , Yuqian Gao , Xiaoyu Xin , Ting Zhu , Lingling Wang , Linsheng Song\",\"doi\":\"10.1016/j.dci.2024.105217\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Norepinephrine (NE) is involved in regulating cytokine expression and phagocytosis of immune cells in the innate immunity of vertebrates. In the present study, the modulation mechanism of NE on the biosynthesis of TNFs in oyster granulocytes was explored. The transcripts of <em>Cg</em>TNF-1, <em>Cg</em>TNF-2 and <em>Cg</em>TNF-3 were highly expressed in granulocytes, and they were significantly up-regulated after LPS stimulation, while down-regulated after NE treatment. The phagocytic rate and apoptosis index of oyster granulocytes were also triggered by LPS stimulation and suppressed by NE treatment. The mRNA expressions of <em>Cg</em>MAPK14 and <em>Cg</em>Relish were significantly induced after NE treatment, and the translocation of <em>Cg</em>Relish from cytoplasm to nucleus was observed. The concentration of intracellular Ca<sup>2+</sup> in granulocytes was significantly up-regulated upon NE incubation, and this trend reverted after the treatment with DOX (specific antagonist for NE receptor, <em>Cg</em>A1AR-1). No obvious significance was observed in intracellular cAMP concentrations in the PBS, NE and NE + DOX groups. Once <em>Cg</em>A1AR-1 was blocked by DOX, the mRNA expressions of <em>Cg</em>MAPK14 and <em>Cg</em>Relish were significantly inhibited, and the translocation of <em>Cg</em>Relish from cytoplasm to nucleus was also dramatically suppressed, while the mRNA expression of <em>Cg</em>TNF-1 and the apoptosis index increased significantly to the same level with those in LPS group, respectively. These results collectively suggested that NE modulated TNF expression in oyster granulocyte through A1AR-p38 MAPK-Relish signaling pathway.</p></div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-06-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0145305X24000892\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0145305X24000892","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
引用次数: 0
摘要
去甲肾上腺素(NE)参与调节脊椎动物先天性免疫中细胞因子的表达和免疫细胞的吞噬作用。本研究探讨了 NE 对牡蛎粒细胞 TNFs 生物合成的调节机制。CgTNF-1、CgTNF-2和CgTNF-3的转录本在粒细胞中高表达,它们在LPS刺激后显著上调,而在NE处理后下调。牡蛎粒细胞的吞噬率和凋亡指数也受 LPS 刺激的触发和 NE 处理的抑制。NE处理后,CgMAPK14和CgRelish的mRNA表达被显著诱导,并观察到CgRelish从细胞质转位到细胞核。NE培养后,粒细胞内Ca2+浓度明显升高,而用DOX(NE受体的特异性拮抗剂,CgA1AR-1)处理后,这一趋势得到恢复。PBS组、NE组和NE+DOX组细胞内cAMP浓度无明显变化。DOX阻断CgA1AR-1后,CgMAPK14和CgRelish的mRNA表达受到显著抑制,CgRelish从细胞质向细胞核的转位也被显著抑制,而CgTNF-1的mRNA表达和细胞凋亡指数则分别显著增加至与LPS组相同的水平。这些结果共同表明,NE通过A1AR-p38 MAPK-Relish信号通路调节了牡蛎粒细胞中TNF的表达。
Norepinephrine regulates TNF expression via the A1AR-p38 MAPK-Relish pathway in granulocytes of oyster Crassostrea gigas
Norepinephrine (NE) is involved in regulating cytokine expression and phagocytosis of immune cells in the innate immunity of vertebrates. In the present study, the modulation mechanism of NE on the biosynthesis of TNFs in oyster granulocytes was explored. The transcripts of CgTNF-1, CgTNF-2 and CgTNF-3 were highly expressed in granulocytes, and they were significantly up-regulated after LPS stimulation, while down-regulated after NE treatment. The phagocytic rate and apoptosis index of oyster granulocytes were also triggered by LPS stimulation and suppressed by NE treatment. The mRNA expressions of CgMAPK14 and CgRelish were significantly induced after NE treatment, and the translocation of CgRelish from cytoplasm to nucleus was observed. The concentration of intracellular Ca2+ in granulocytes was significantly up-regulated upon NE incubation, and this trend reverted after the treatment with DOX (specific antagonist for NE receptor, CgA1AR-1). No obvious significance was observed in intracellular cAMP concentrations in the PBS, NE and NE + DOX groups. Once CgA1AR-1 was blocked by DOX, the mRNA expressions of CgMAPK14 and CgRelish were significantly inhibited, and the translocation of CgRelish from cytoplasm to nucleus was also dramatically suppressed, while the mRNA expression of CgTNF-1 and the apoptosis index increased significantly to the same level with those in LPS group, respectively. These results collectively suggested that NE modulated TNF expression in oyster granulocyte through A1AR-p38 MAPK-Relish signaling pathway.