用于细胞成分高级分类的紧凑型林奈克式高光谱定量相显微镜。

IF 2 3区 物理与天体物理 Q3 BIOCHEMICAL RESEARCH METHODS
Himanshu Joshi, Bhanu Pratap Singh, Ankit Butola, Varun Surya, Deepika Mishra, Krishna Agarwal, Dalip Singh Mehta
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引用次数: 0

摘要

高光谱定量相位显微镜(HS-QPM)涉及窄光谱带相位图像的采集,可对不同的生物样本进行波长依赖性研究。本研究开发了一种紧凑型林奈克式 HS-QPM 系统,以降低传统 HS-QPM 技术的不稳定性和复杂性。参比臂和样品臂均使用单物镜,使得该装置结构紧凑。通过评估工业和生物样本的 HS 相图,展示了该系统的能力。不同波长的脱落颊细胞相位图被叠加在一起,形成一个 HS 相位立方体,为空间相位分布增加了光谱维度。利用主成分分析法对不同细胞成分的波长响应进行分析,以确定 HS 相位数据集中的主要光谱特征。研究结果强调了 HS-QPM 在推进生物医学研究和临床应用的细胞表征方面的效率和有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Compact Linnik-type hyperspectral quantitative phase microscope for advanced classification of cellular components

Compact Linnik-type hyperspectral quantitative phase microscope for advanced classification of cellular components

Hyperspectral quantitative phase microscopy (HS-QPM) involves the acquisition of phase images across narrow spectral bands, which enables wavelength-dependent study of different biological samples. In the present work, a compact Linnik-type HS-QPM system is developed to reduce the instability and complexity associated with conventional HS-QPM techniques. The use of a single objective lens for both reference and sample arms makes the setup compact. The capabilities of the system are demonstrated by evaluating the HS phase map of both industrial and biological specimens. Phase maps of exfoliated cheek cells at different wavelengths are stacked to form a HS phase cube, adding spectral dimensionality to spatial phase distribution. Analysis of wavelength response of different cellular components are performed using principal component analysis to identify dominant spectral features present in the HS phase dataset. Findings of the study emphasize on the efficiency and effectiveness of HS-QPM for advancing cellular characterization in biomedical research and clinical applications.

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来源期刊
Journal of Biophotonics
Journal of Biophotonics 生物-生化研究方法
CiteScore
5.70
自引率
7.10%
发文量
248
审稿时长
1 months
期刊介绍: The first international journal dedicated to publishing reviews and original articles from this exciting field, the Journal of Biophotonics covers the broad range of research on interactions between light and biological material. The journal offers a platform where the physicist communicates with the biologist and where the clinical practitioner learns about the latest tools for the diagnosis of diseases. As such, the journal is highly interdisciplinary, publishing cutting edge research in the fields of life sciences, medicine, physics, chemistry, and engineering. The coverage extends from fundamental research to specific developments, while also including the latest applications.
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