外周膜结合的 Tam41 型酶合成 CDP-DAG。

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Koji Okamoto
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引用次数: 0

摘要

胞苷二磷酸二酰甘油(CDP-DAG)是原核生物和真核生物转化为多种磷脂的关键中间体。在芽殖酵母中,CDP-DAG 由三磷酸胞苷(CTP)和磷脂酸(PA)合成,由内质网中的膜整合蛋白 Cds1 和线粒体中的外周膜结合蛋白 Tam41 催化。尽管最近的一项研究发现,裂殖酵母的 SpTam41 由一个核苷酸转移酶结构域和一个翼状螺旋结构域组成,在两个结构域之间形成一个用于结合 CTP 的活性位点口袋,C-末端的两性螺旋用于与膜结合,但 CTP 和 Mg2+(最喜欢的二价阳离子)如何与 PA 相容仍不清楚。最近,Kimura 等人的报告(J. Biochem. 2022; 171:429-441)解决了 FbTam41(一种来自真菌的功能直向同源物)与 CTP-Mg2+ 的晶体结构,成功地提供了 CDP-DAG 合成的详细分子观点。在这篇评论中,我们讨论了目前对 Tam41 介导的反应的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CDP-DAG synthesis by peripheral membrane-bound Tam41-type enzymes.

Cytidine diphosphate diacylglycerol (CDP-DAG) is a critical intermediate that is converted to multiple phospholipids in prokaryotes and eukaryotes. In budding yeast, CDP-DAG synthesis from cytidine triphosphate (CTP) and phosphatidic acid (PA) is catalyzed by the membrane-integrated protein Cds1 in the endoplasmic reticulum and the peripheral membrane-bound protein Tam41 in mitochondria. Although a recent study revealed that the fission yeast SpTam41 consists of a nucleotidyltransferase domain and a winged helix domain, forming an active-site pocket for CTP binding between the two domains together with a C-terminal amphipathic helix for membrane association, how CTP and Mg 2+, a most-favoured divalent cation, are accommodated with PA remains obscure. A more recent report by Kimura et al. (J. Biochem. 2022; 171:429-441) solved the crystal structure of FbTam41, a functional ortholog from a Firmicutes bacterium, with CTP-Mg 2+, successfully providing a detailed molecular view of CDP-DAG synthesis. In this commentary, our current understanding of Tam41-mediated reaction is discussed.

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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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