通过 HRP 自偶联增强氧化铱纳米粒子聚集检测非小细胞肺癌外泌体的可视化双模式灵敏传感器。

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Xin Xu, Ze Zhang, Tong Shen, Hongzhi Pan, Dong Chang
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引用次数: 0

摘要

非小细胞肺癌死亡率高的主要原因是,患者通常在疾病晚期才被确诊。外泌体是正常细胞或肿瘤细胞分泌的小膜囊泡,在非小细胞肺癌的进展过程中起着重要作用。本研究成功优化了与辣根过氧化物酶自偶联的人工纳米酶(IrO2NPs@HRP-AptCD63)的制备方法,在不添加任何配体的情况下,显示出显著的催化活性,适用于检测NSCLC外泌体表面的表皮生长因子受体蛋白。IrO2NPs@HRP在3,3',5,5'-四甲基联苯胺-H2O2氧化还原体系中表现出更强的催化活性,从而增强了比色信号。这种现象可以用肉眼分辨,也可以用紫外可见分光光度计进行量化。同时,随着氧化还原反应的发生,作为电解质的 3,3',5,5'-四甲基联苯胺-H2O2 的电流信号也会发生变化。所开发的灵敏传感器可产生双模信号输出,首先可目测样品的阳性或阴性状态,然后采用更深入的电化学或比色分析方法,对可疑的阳性样品进行详细的定量分析。电化学分析和比色分析的检测限分别为 0.9 × 103 粒子/毫升和 0.14 × 103 粒子/毫升。与CA125等传统生物标记物相比,该方法具有极高的特异性,能同时区分健康志愿者、慢性阻塞性肺病患者和NSCLC患者的血清外泌体,促进了小鼠模型中外泌体的检测,从而用于肿瘤监测。此外,它还阐明了血清外泌体表面表皮生长因子受体蛋白表达在整个发育过程中的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Visual dual-mode aptasensor for non-small cell lung cancer exosome detection via HRP self-coupling enhanced oxidized iridium nanoparticle aggregation

The main reason for the high mortality rate of non-small cell lung cancer is that patients are usually diagnosed at an advanced stage of the disease. Exosomes, small membrane vesicles secreted by normal cells or tumor cells, play a significant role in the progression of NSCLC. This study successfully optimized the preparation of artificial nanoenzymes self-coupling with horseradish peroxidase (IrO2NPs@HRP-AptCD63), without adding any ligand, demonstrating remarkable catalytic activity suitable for detecting the EGFR protein on the surface of NSCLC exosomes. When fused with the CD63 aptamer for identifying NSCLC exosomes, IrO2NPs@HRP showed enhanced catalytic activity in the 3,3′,5,5′-tetramethylbenzidine-H2O2 oxidation–reduction system, thereby enhancing the colorimetric signal. This phenomenon can be distinguished by the naked eye and quantified using a UV–Vis spectrophotometer. Meanwhile, as the redox reaction occurs, the current signal of 3,3′,5,5′-tetramethylbenzidine-H2O2, acting as an electrolyte, changes. The developed aptasensor generates dual-mode signal outputs, firstly, to visually assess the samples for their positive or negative status, and subsequently employ more in-depth electrochemical or colorimetric analysis methods for a detailed quantitative analysis of suspected positive samples. The detection limits of electrochemical analysis and colorimetric analysis were 0.9 × 10particles/mL and 0.14 × 10particles/mL, respectively. Compared with traditional biomarkers such as CA125, this method exhibits exceptional specificity, capable of simultaneously distinguishing serum exosomes of healthy volunteers, COPD patients, and NSCLC patients, promoting exosome detection in mouse models for tumor monitoring. Additionally, it elucidates the changes in EGFR protein expression on the surface of serum exosomes throughout the developmental trajectory.

Graphical abstract

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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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