Shin-Mei Yeh, Minsoo Yoon, Sidney Scott, Abhishek Chatterjee, Lauren M Hemara, Ronan K Y Chen, Tianchi Wang, Kerry Templeton, Erik H A Rikkerink, Jay Jayaraman, Cyril Brendolise
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引用次数: 0
摘要
Pseudomonas syringae pv. actinidiae biovar 3(Psa3)会导致黄肉猕猴桃(Actinidia chinensis)发生毁灭性腐烂病。在导致猕猴桃腐烂病全球大爆发的所有 Psa3 分离物中都存在效应子 HopZ5,它能触发本苋属烟草的免疫,但在易感猕猴桃栽培品种中却无法识别。在寻找 N. benthamiana 对 HopZ5 的非宿主抗性基因时,我们发现核苷酸结合富亮氨酸重复受体 NbPTR1 能识别 HopZ5。来自 N. benthamiana 和 A. chinensis 的 RPM1-interacting protein 4 同源物与 NbPTR1 形成复合物,而 HopZ5 的活性能够破坏这种相互作用。在 chinensis 中没有发现 NbPTR1 的功能性直向同源物。将 NbPTR1 转化到对 Psa3 敏感的 A. chinensis var.总之,这项研究表明,在易感 Psa3 的猕猴桃中表达 NbPTR1 是获得 Psa3 抗性的一种可行方法,它为其他易感猕猴桃基因型的抗性工程提供了有价值的信息。
NbPTR1 confers resistance against Pseudomonas syringae pv. actinidiae in kiwifruit.
Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes a devastating canker disease in yellow-fleshed kiwifruit (Actinidia chinensis). The effector HopZ5, which is present in all isolates of Psa3 causing global outbreaks of pandemic kiwifruit canker disease, triggers immunity in Nicotiana benthamiana and is not recognised in susceptible A. chinensis cultivars. In a search for N. benthamiana nonhost resistance genes against HopZ5, we found that the nucleotide-binding leucine-rich repeat receptor NbPTR1 recognised HopZ5. RPM1-interacting protein 4 orthologues from N. benthamiana and A. chinensis formed a complex with NbPTR1 and HopZ5 activity was able to disrupt this interaction. No functional orthologues of NbPTR1 were found in A. chinensis. NbPTR1 transformed into Psa3-susceptible A. chinensis var. chinensis 'Hort16A' plants introduced HopZ5-specific resistance against Psa3. Altogether, this study suggested that expressing NbPTR1 in Psa3-susceptible kiwifruit is a viable approach to acquiring resistance to Psa3 and it provides valuable information for engineering resistance in otherwise susceptible kiwifruit genotypes.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.