评估负载 EGCG 的纳米颗粒在未受刺激和受刺激小鼠中的抗抑郁潜力

Shakti Dahiya, Ruma Rani, Neeraj Dilbaghi, Dinesh Dhingra, Sant Lal and Jaya Verma
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引用次数: 0

摘要

表没食子儿茶素-3-棓酸盐(EGCG)是绿茶中的一种主要生物活性成分,在实验动物中显示出显著的抗抑郁活性。纳米微粒给药具有巨大潜力,可通过将 EGCG 转化为有效的可给药药物,克服其溶解度低和稳定性差等缺点。在目前的研究中,我们采用反溶剂沉淀法合成了EGCG单独和与胡椒碱的纳米制剂,然后评估了它们在未受应激和受应激的瑞士雄性白化小鼠体内的抗抑郁效果。小鼠暴露于不同的应激源,如捏尾巴、诱导固定等,时间跨度为三周。Zein是一种蛋白质纳米载体,与EGCG(25毫克)和EGCG+胡椒碱(25毫克+5毫克)一起被纳米封装。连续三周给小鼠服用含 EGCG 的纳米悬浮剂(25 毫克/公斤-1)和 EGCG-胡椒碱纳米复合物(25 毫克/公斤-1)。为了确定各种药物治疗对应激和非应激小鼠的影响,采用了尾悬试验(TST)作为行为范例。同时还评估了暴露于各种药物处理的小鼠对运动活动的影响。将动物安乐死后,进一步测定血浆皮质酮、血浆亚硝酸盐、脑丙二醛、脑 MAO-A、脑还原型谷胱甘肽和脑过氧化氢酶的水平。与使用药物治疗的对照组相比,EGCG-哌啶纳米复合物(25 毫克/千克-1)和盐酸帕罗西汀(10 毫克/千克-1)可显著缩短未受刺激和受刺激小鼠的静止时间。但是,在运动活动方面,没有观察到明显的影响。EGCG纳米悬浮剂、EGCG-哌啶纳米复合物和盐酸帕罗西汀能显著降低血浆亚硝酸盐、脑MAO-A、脑丙二醛和脑过氧化氢酶的水平。然而,与使用药物治疗的对照组相比,这些药物治疗可明显提高未应激和应激小鼠的血浆皮质酮和脑还原型谷胱甘肽水平。因此,腹腔注射单独使用 EGCG 和与胡椒碱一起合成的纳米制剂可明显改善小鼠的抗抑郁行为。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Evaluation of the anti-depressant potential of EGCG-loaded nanoparticles in unstressed and stressed mice

Evaluation of the anti-depressant potential of EGCG-loaded nanoparticles in unstressed and stressed mice

Epigallocatechin-3-gallate (EGCG) is a key bio-active component of green tea and has demonstrated significant antidepressant activity in laboratory animals. Nano-particulate drug delivery offers great potential to overcome drawbacks associated with EGCG i.e. its low solubility and stability by transforming it into effective deliverable drugs. In the current study, nano-formulations of EGCG alone and with piperine were synthesized using antisolvent precipitation methodology followed by evaluation of their in vivo antidepressant effect in unstressed and stressed Swiss male albino mice. The mice were exposed to distinct stressors i.e. tail pinch, induction of immobilization, etc. throughout a span of three weeks. Zein, a protein nanocarrier, was nano-encapsulated with EGCG (25 mg) and EGCG + piperine (25 mg + 5 mg). For a continuous three weeks, the mice were administered EGCG-loaded nanosuspensions (25 mg kg−1) and EGCG–piperine nanocomplexes (25 mg kg−1). To determine the impact of various medication treatments on stressed and unstressed mice, the tail suspension test (TST) was employed as a behavioural paradigm. Mice exposed to various drug treatments were also evaluated for the effect on locomotor activity. The animals were euthanized followed by further estimation of plasma corticosterone, plasma nitrite, brain malondialdehyde, brain MAO-A, brain reduced glutathione, and brain catalase levels. The EGCG–piperine nanocomplex (25 mg kg−1) and paroxetine HCl (10 mg kg−1) per se significantly reduced immobility time in unstressed and stressed mice as compared to their respective control groups treated with a vehicle. However, in the case of locomotor activity, no significant effect was observed. EGCG loaded nanosuspension, EGCG–piperine nanocomplex and paroxetine HCl significantly decreased plasma nitrite, and brain MAO-A, brain malondialdehyde and brain catalase levels. However, these drug treatments significantly increased plasma corticosterone and brain reduced glutathione levels in unstressed and stressed mice as compared to their respective control groups treated with a vehicle. So, the intraperitoneal administration of nanoformulations synthesized using EGCG alone and along with piperine significantly improves the antidepressant-like behavior in mice.

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