假单胞菌 WBC-3 的蛋白 1619 通过将对苯醌转化为对苯二酚参与对硝基苯酚降解过程

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhongchan Peng , Wenxian Zhang , Yishuang Duan , Jing Gu , Jiaoyu Deng
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引用次数: 0

摘要

由于对硝基苯酚(PNP)具有剧毒,而且能够在环境中持久存在,因此有必要开发适当的方法来消除环境中的对硝基苯酚。此前,从污染土壤中分离出的假单胞菌 WBC-3 菌株被发现能够利用对硝基苯酚作为唯一的碳源和氮源,但效率不高。在本研究中,通过从头开始的基因组测序,WBC-3 被证明属于假单胞菌(Pseudomonas putida)。为了提高其利用 PNP 的效率,研究人员获得了一株 PNP 降解率显著提高的突变菌株(PM1-33)。虽然在 WBC-3 和 PM1-33 之间没有观察到已知 PNP 分解基因/蛋白质表达水平的增加,但在 PM1-33 中,蛋白质 1619 的表达水平明显增加。在 WBC-3 和 PM1-33 中删除 GM1619 会导致两种菌株的 PNP 降解率下降,并消除两种菌株之间的 PNP 降解差异。使用 AlphaFold2 进行的功能预测显示,蛋白质 1619 可能与对苯醌(BQ)结合。因此,对蛋白 1619 进行了生物化学鉴定,证实了其将对苯醌(BQ)转化为对苯二酚(HQ)的能力。因此,发现了一种参与 PNP 降解的新蛋白,从而为细菌的 PNP 降解途径增添了新的知识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Protein 1619 of Pseudomonas putida WBC-3 participates in para-nitrophenol degradation by converting p-benzoquinone to hydroquinone

It is necessary to develop appropriate approaches to eliminate para-nitrophenol (PNP) in our environment, because the pollutant is highly toxic and also able to persist in the environment. Previously, Pseudomonas sp. strain WBC-3 isolated from polluted soil was found to be able to use PNP as the sole carbon and nitrogen source, but not very efficiently. In this study, WBC-3 was shown to belong to Pseudomonas putida through de novo genome sequencing. To enhance its efficiency of PNP utilization, a mutant strain (PM1-33) with a significantly increased PNP degradation rate was obtained. Although no increase in the expression levels of known PNP catabolizing genes/proteins were observed between WBC-3 and PM1-33, the expression level of protein 1619 significantly increased in PM1-33. Deleting GM1619 in WBC-3 and PM1-33 caused decreased PNP degradation rates in both strains and eliminated the difference in PNP degradation between the two strains. Functional prediction using AlphaFold2 showed that protein1619 might bind to p-benzoquinone (BQ). Consequently, protein 1619 was biochemically characterized, confirming its ability to convert BQ into hydroquinone (HQ). Thus, a new protein involved in PNP degradation was identified, thereby adding new knowledge to bacterial PNP degradation pathways.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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