{"title":"通过对乙酰氨基酚的解毒作用了解新型 Scenedesmus 菌株的生化转变:利用方框-贝肯优化和等温线分析进行实验","authors":"Dixita Phukan, Vipin Kumar, Ankur Singh, Saumya Anand","doi":"10.1016/j.ibiod.2024.105841","DOIUrl":null,"url":null,"abstract":"<div><p>Acetaminophen's inherent solubility and hydrophilic nature facilitate its accumulation in aquatic ecosystems. Herein, <em>Scenedesmus dimorphus</em> IITISM-DIX1 demonstrates efficient acetaminophen removal, concurrently serving as a substrate for lipid biosynthesis. Employing Box-Behnken design, optimization of parameters like pH, light duration and concentration of acetaminophen influencing its elimination is executed. Characterization of pre- and post-algal biomass involves FE-SEM, FTIR, and BET analysis. Kinetic and adsorption analyses reveal pseudo-first-order kinetics (R<sup>2</sup> = 0.99) and adherence to the Freundlich isotherm (R<sup>2</sup> = 0.94). FTIR spectroscopy demonstrates subtle shifts in IR bands post-sorption, indicative of biomass involvement in adsorption processes. Biodegradation and biosorption serve as the main removal pathways, facilitated by exopolysaccharides, generating by-products such as 4-aminophenol, hydroquinone, and formic acid. The Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC) values obtained for the Freundlich isotherm validate it as the optimal model, indicating heterogeneous multilayered sorption with efficiency ranging from 44% to 100%. Additionally, exposure to acetaminophen-contaminated media leads to biochemical alterations in <em>Scenedesmus dimorphus</em> IITISM-DIX1. The findings of this study unveil the first elucidated pathway for acetaminophen degradation by any <em>Scenedesmus</em> species, delivering essential knowledge about microalgae-mediated acetaminophen degradation and lipid enrichment mechanisms.</p></div>","PeriodicalId":13643,"journal":{"name":"International Biodeterioration & Biodegradation","volume":"193 ","pages":"Article 105841"},"PeriodicalIF":4.1000,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Accessing biochemical shifts in a novel Scenedesmus strain via acetaminophen detoxification: Experiment utilizing Box-Behnken optimization and isotherm analysis\",\"authors\":\"Dixita Phukan, Vipin Kumar, Ankur Singh, Saumya Anand\",\"doi\":\"10.1016/j.ibiod.2024.105841\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Acetaminophen's inherent solubility and hydrophilic nature facilitate its accumulation in aquatic ecosystems. Herein, <em>Scenedesmus dimorphus</em> IITISM-DIX1 demonstrates efficient acetaminophen removal, concurrently serving as a substrate for lipid biosynthesis. Employing Box-Behnken design, optimization of parameters like pH, light duration and concentration of acetaminophen influencing its elimination is executed. Characterization of pre- and post-algal biomass involves FE-SEM, FTIR, and BET analysis. Kinetic and adsorption analyses reveal pseudo-first-order kinetics (R<sup>2</sup> = 0.99) and adherence to the Freundlich isotherm (R<sup>2</sup> = 0.94). FTIR spectroscopy demonstrates subtle shifts in IR bands post-sorption, indicative of biomass involvement in adsorption processes. Biodegradation and biosorption serve as the main removal pathways, facilitated by exopolysaccharides, generating by-products such as 4-aminophenol, hydroquinone, and formic acid. The Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC) values obtained for the Freundlich isotherm validate it as the optimal model, indicating heterogeneous multilayered sorption with efficiency ranging from 44% to 100%. Additionally, exposure to acetaminophen-contaminated media leads to biochemical alterations in <em>Scenedesmus dimorphus</em> IITISM-DIX1. The findings of this study unveil the first elucidated pathway for acetaminophen degradation by any <em>Scenedesmus</em> species, delivering essential knowledge about microalgae-mediated acetaminophen degradation and lipid enrichment mechanisms.</p></div>\",\"PeriodicalId\":13643,\"journal\":{\"name\":\"International Biodeterioration & Biodegradation\",\"volume\":\"193 \",\"pages\":\"Article 105841\"},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-06-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Biodeterioration & Biodegradation\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0964830524001124\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Biodeterioration & Biodegradation","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0964830524001124","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Accessing biochemical shifts in a novel Scenedesmus strain via acetaminophen detoxification: Experiment utilizing Box-Behnken optimization and isotherm analysis
Acetaminophen's inherent solubility and hydrophilic nature facilitate its accumulation in aquatic ecosystems. Herein, Scenedesmus dimorphus IITISM-DIX1 demonstrates efficient acetaminophen removal, concurrently serving as a substrate for lipid biosynthesis. Employing Box-Behnken design, optimization of parameters like pH, light duration and concentration of acetaminophen influencing its elimination is executed. Characterization of pre- and post-algal biomass involves FE-SEM, FTIR, and BET analysis. Kinetic and adsorption analyses reveal pseudo-first-order kinetics (R2 = 0.99) and adherence to the Freundlich isotherm (R2 = 0.94). FTIR spectroscopy demonstrates subtle shifts in IR bands post-sorption, indicative of biomass involvement in adsorption processes. Biodegradation and biosorption serve as the main removal pathways, facilitated by exopolysaccharides, generating by-products such as 4-aminophenol, hydroquinone, and formic acid. The Akaike Information Criterion (AIC) and Bayesian Information Criterion (BIC) values obtained for the Freundlich isotherm validate it as the optimal model, indicating heterogeneous multilayered sorption with efficiency ranging from 44% to 100%. Additionally, exposure to acetaminophen-contaminated media leads to biochemical alterations in Scenedesmus dimorphus IITISM-DIX1. The findings of this study unveil the first elucidated pathway for acetaminophen degradation by any Scenedesmus species, delivering essential knowledge about microalgae-mediated acetaminophen degradation and lipid enrichment mechanisms.
期刊介绍:
International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.