热处理木材提取物对木材腐朽真菌的抗真菌活性

IF 4.1 2区 环境科学与生态学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zhenju Bi , Xinqi Gao , Jing Zhang , Yafang Lei , Li Yan
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引用次数: 0

摘要

利用热处理保护木材的方法已趋于成熟,但热处理木材提取物在抗腐烂方面的作用却缺乏关注。为了评估热处理木材提取物在木材防腐方面的潜力,我们测试了提取物对木材腐朽真菌的抗真菌活性以及提取物对真菌木材降解酶活性和细胞膜完整性的影响。对落叶松(Larix gmelinii (Rupr.) Kuzen)加工过程中产生的小刨花进行了热处理和提取。测试了萃取物对木材腐朽真菌的抗真菌活性以及浸渍萃取物木材的抗腐朽性,以评估其潜力。通过检测与提取物培养的真菌的纤维素酶、半纤维素酶和木质素酶的活性,评估了提取物对白腐真菌 Trametes versicolor (L.) Lloyd 和褐腐真菌 Gloeophyllum trabeum (Pers.: Fr.) Murr.萃取物对真菌细胞膜完整性的影响是通过碘化丙啶(PI)染色和真菌暴露于萃取物后的核酸和蛋白质泄漏检测来评估的。测试了浸渍提取物的木材浸出液对淡水发光细菌(青海弧菌-Q67)和小鼠巨噬细胞(RAW264.7)的毒性,并与原木浸出液进行了比较。结果表明,经热处理的落叶松木材提取物可提高杨木(Populus tomentosa Carr.)的抗腐性能,且浸渍木材的提取物浸出液对 Q67 和 RAW264.7 的影响与原木材浸出液相同。提取物抑制了受试真菌的木质素酶活性(仅对 T. versicolor)、纤维素酶活性和呼吸代谢,并损害了膜的完整性。这项研究发现了一种潜在的木材防腐剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antifungal activity of heat-treated wood extract against wood decay fungi

Antifungal activity of heat-treated wood extract against wood decay fungi

Using heat treatment for wood protection has been driven to maturity, but the role of heat-treated wood extracts in decay resistance has lacked attention. To assess the potential of heat-treated wood extract for wood preservation, the antifungal activity of the extract against wood decay fungi and the effects of the extract on fungal wood degrading enzyme activity and cell membrane integrity were tested. Small shavings generated during the processing of larch (Larix gmelinii (Rupr.) Kuzen) were heat-treated and extracted. The antifungal activity of extract against wood decay fungi and decay resistance of extract impregnated wood were tested to assess their potential. The effect of extracts on the enzyme activity of the white-rot fungus, Trametes versicolor (L.) Lloyd and the brown-rot fungus, Gloeophyllum trabeum (Pers.: Fr.) Murr. for wood degradation was assessed by detecting the activity of cellulose, hemicellulose, and ligninase of fungi incubated with extract. The effect of extracts on the integrity of cell membranes of fungi was assessed by staining with propidium iodide (PI) and the leakage detection of nucleic acid and protein in fungi after exposure to extract. The toxicity to freshwater luminescent bacteria (Vibrio qinghaiensis sp. -Q67) and mouse macrophages (RAW264.7) of the extract impregnated wood leachate was tested and compared with the leachate of the raw wood. The results denoted that the decay resistance of poplar (Populus tomentosa Carr.) wood could be improved by heat-treated larch wood extract, and the effect of the extract impregnated wood leachate on Q67 and RAW264.7 was the same as that of raw wood leachate. The extract inhibited ligninase activity (only for T. versicolor), cellulase activity, and respiratory metabolism of tested fungi, and impaired the membrane integrity. The study identified a potential wood preservative.

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来源期刊
CiteScore
9.60
自引率
10.40%
发文量
107
审稿时长
21 days
期刊介绍: International Biodeterioration and Biodegradation publishes original research papers and reviews on the biological causes of deterioration or degradation.
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