DNA-PAINT 适配器可实现高效的多路复用。

IF 4.3 Q1 BIOCHEMICAL RESEARCH METHODS

Cell Reports Methods Pub Date : 2024-06-17 DOI:10.1016/j.crmeth.2024.100801

Matthew D Lycas, Suliana Manley

{"title":"DNA-PAINT 适配器可实现高效的多路复用。","authors":"Matthew D Lycas, Suliana Manley","doi":"10.1016/j.crmeth.2024.100801","DOIUrl":null,"url":null,"abstract":"Multiplexed super-resolution imaging offers a route to spatial proteomics; however, time-efficient mapping of many protein species has been challenging. Two recent works in Cell highlight SUM-PAINT and FLASH-PAINT, methods that leverage adaptor DNA strand design to combine advances in multiplexing with increases in speed of label exchange. These advances permit unbiased omics-style analyses to advance biological insights from super-resolution images.","PeriodicalId":29773,"journal":{"name":"Cell Reports Methods","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11228366/pdf/","citationCount":"0","resultStr":"{\"title\":\"DNA-PAINT adaptors make for efficient multiplexing.\",\"authors\":\"Matthew D Lycas, Suliana Manley\",\"doi\":\"10.1016/j.crmeth.2024.100801\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Multiplexed super-resolution imaging offers a route to spatial proteomics; however, time-efficient mapping of many protein species has been challenging. Two recent works in Cell highlight SUM-PAINT and FLASH-PAINT, methods that leverage adaptor DNA strand design to combine advances in multiplexing with increases in speed of label exchange. These advances permit unbiased omics-style analyses to advance biological insights from super-resolution images.\",\"PeriodicalId\":29773,\"journal\":{\"name\":\"Cell Reports Methods\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11228366/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cell Reports Methods\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.crmeth.2024.100801\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell Reports Methods","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.crmeth.2024.100801","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}

引用次数: 0

摘要

多路复用超分辨率成像为空间蛋白质组学提供了一条途径；然而，许多蛋白质种类的时间效率绘图一直是个挑战。最近发表在《细胞》（Cell）杂志上的两篇论文重点介绍了SUM-PAINT和FLASH-PAINT，这两种方法利用适配器DNA链的设计，将多路复用技术的进步与标签交换速度的提高结合起来。这些进步使得无偏见的omics式分析得以从超分辨率图像中推进生物洞察力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

DNA-PAINT adaptors make for efficient multiplexing.

Multiplexed super-resolution imaging offers a route to spatial proteomics; however, time-efficient mapping of many protein species has been challenging. Two recent works in Cell highlight SUM-PAINT and FLASH-PAINT, methods that leverage adaptor DNA strand design to combine advances in multiplexing with increases in speed of label exchange. These advances permit unbiased omics-style analyses to advance biological insights from super-resolution images.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Cell Reports Methods Chemistry (General), Biochemistry, Genetics and Molecular Biology (General), Immunology and Microbiology (General)

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

111 days