利用酵母研究蛋白质进化中的非功能性因素。

IF 2.2 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yeast Pub Date : 2024-09-01 Epub Date: 2024-06-19 DOI:10.1002/yea.3970
Katarzyna Potera, Katarzyna Tomala
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引用次数: 0

摘要

驱动蛋白质序列进化的不仅有与蛋白质功能和形状直接相关的因素,还有非功能性因素。蛋白质进化中的这些因素可分为与能量成本、合成效率以及避免错误折叠和毒性有关的因素。研究这些因素的常见方法是进行相关分析,将其与蛋白质的某些特征(如氨基酸组成)进行对比,但这些特征是相互依存的。为了避免可能出现的偏差,需要进行实证研究,但迄今为止这方面的工作还不够多。在这篇综述中,我们描述了非功能因子在蛋白质进化中的作用,并提出了一种以酵母为合适模式生物的实验方法。该方法的重点在于改变与功能无关的蛋白质特性的突变对适宜性的潜在负面影响,以及改变这些特性的突变的频率。以避免错误折叠假说来解释高表达蛋白质进化缓慢的实验结果与相关研究结果不一致。因此,应该做出更多努力,对蛋白质进化中的非功能性因素的影响进行实证检验,并将这些结果与相关分析方法的结果进行对比。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using yeasts for the studies of nonfunctional factors in protein evolution.

The evolution of protein sequence is driven not only by factors directly related to protein function and shape but also by nonfunctional factors. Such factors in protein evolution might be categorized as those connected to energetic costs, synthesis efficiency, and avoidance of misfolding and toxicity. A common approach to studying them is correlational analysis contrasting them with some characteristics of the protein, like amino acid composition, but these features are interdependent. To avoid possible bias, empirical studies are needed, and not enough work has been done to date. In this review, we describe the role of nonfunctional factors in protein evolution and present an experimental approach using yeast as a suitable model organism. The focus of the proposed approach is on the potential negative impact on the fitness of mutations that change protein properties not related to function and the frequency of mutations that change these properties. Experimental results of testing the misfolding avoidance hypothesis as an explanation for why highly expressed proteins evolve slowly are inconsistent with correlational research results. Therefore, more efforts should be made to empirically test the effects of nonfunctional factors in protein evolution and to contrast these results with the results of the correlational analysis approach.

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来源期刊
Yeast
Yeast 生物-生化与分子生物学
CiteScore
5.30
自引率
3.80%
发文量
55
审稿时长
3 months
期刊介绍: Yeast publishes original articles and reviews on the most significant developments of research with unicellular fungi, including innovative methods of broad applicability. It is essential reading for those wishing to keep up to date with this rapidly moving field of yeast biology. Topics covered include: biochemistry and molecular biology; biodiversity and taxonomy; biotechnology; cell and developmental biology; ecology and evolution; genetics and genomics; metabolism and physiology; pathobiology; synthetic and systems biology; tools and resources
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