从纺织废水中分离出的 Caprae 葡萄球菌对 Reactive Red-195 偶氮染料的脱醇和解毒作用。

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Monika Yadav, Asha Lata Singh
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引用次数: 0

摘要

纺织业大量使用偶氮染料作为染色剂。因此,会产生大量富含染料的废水,进而造成环境问题。事实证明,利用含有偶氮还原酶的细菌进行生物处理,能更有效地处理染料废水。目前的工作重点是用 Caprae 葡萄球菌(S. caprae)降解和脱色 Reactive Red-195 (RR-195) 偶氮染料。为此,对 pH 值、温度、接种物、碳源和氮源以及染料浓度等因素进行了优化,以实现最大程度的脱色和降解。在浓度为 100 µg/mL、温度为 30 °C、pH 值为 7.0、接触时间为 12 小时的静态条件下,S. caprae(4 mg/mL)能有效地使 RR-195 染料脱色 90%。傅立叶变换红外光谱分析显示,处理后的染料中形成了新的官能团,如 3370 cm-1 的 O-H 伸展带、2928 cm-1 的 C-H 伸展带和 1608 cm-1 的新带,其中 1608 cm-1 代表芳香环的降解,1382 和 1118 cm-1 代表脱硫峰。经鉴定,RR-195 染料的生物降解代谢物(如苯酚、3, 5-二叔丁基苯酚和邻苯二甲酸)可用于工业。植物毒性测试表明,经处理的染料对 Vigna radiata 和 Triticum aestivum 种子的萌发无毒性影响。此外,抗生素扩散试验也证实了 S. caprae 的生物安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Decolourization and detoxification of Reactive Red-195 azo dye by Staphylococcus caprae isolated from textile effluent.

Decolourization and detoxification of Reactive Red-195 azo dye by Staphylococcus caprae isolated from textile effluent.

Azo dyes are used as coloring agent in textile industries at larger scale. As a result, large quantity of dye-enriched waste water is generated which subsequently poses environmental problems. Biological tool involving bacteria having azoreductase enzyme has proved to be more effective and efficient in dye effluent treatment. Current work focuses on Staphylococcus caprae (S. caprae) for degradation and decolorization of Reactive Red-195 (RR-195) azo dye. For this purpose, factors such as pH, temperature, inoculums, carbon and nitrogen sources, and dye concentrations have been optimized for maximum decolorization and degradation. S. caprae (4 mg/mL) efficiently resulted into 90% decolorization of RR-195 dye under static condition at 100 µg/mL concentration, 30 °C and pH 7.0 at a 12-h contact period. FTIR analysis has revealed the formation of new functional groups in the treated dye such as O-H stretch at 3370 cm-1, C-H band stretching at 2928 cm-1, and new band at 1608 cm-1 which specify the degradation of aromatic ring, 1382 and 1118 cm-1 represents desulfonated peaks. Biodegraded metabolites of RR-195 dye such as phenol, 3, 5-di-tert-butylphenol, and phthalic acid have been identified respectively that find industrial applications. Phytotoxicity test has shown non-toxic effects of treated dye on germination of Vigna radiata and Triticum aestivum seeds. Further, antibiotic diffusion assay has confirmed the biosafety of S. caprae.

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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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