阿米替林和胆钙化醇通过调节miR200/BMP4/Olig-2信号转导改善Wistar大鼠应激时的海马组织学结构和髓鞘化。

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Marian Maher Salib Roushdy, Jolly M. W. Labib, Dina Sayed Abdelrahim, Dalia Abdel Wahab Mohamed, Marian Farid Louka Abdelmalak
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引用次数: 0

摘要

慢性压力是一种普遍存在的状况,通常与许多精神疾病相关。大量证据讨论了慢性应激暴露对海马的影响,然而,其潜在的分子通路仍有待确定。我们研究了慢性应激对 miR200/BMP/Olig-2 信号传导和海马髓鞘化的影响。我们还比较了长期服用阿米替林和胆钙化醇对慢性应激海马的影响。与未经处理的慢性应激大鼠相比,阿米替林和胆钙化醇都能显著降低血清皮质醇水平,减少强迫游泳试验中的不动时间,增加开放场试验中的交叉方格数,降低海马中骨形态发生蛋白4(BMP4)的表达及其信使RNA(mRNA)水平,减少miR200的表达。此外,这两种药物都减轻了海马神经元的损伤,增加了存活细胞数量,并增加了Cornu Ammonis亚区1(CA1)锥体层厚度和齿状回颗粒细胞层厚度。与阿米替林相比,胆钙化醇能更有效地增加慢性应激暴露大鼠海马中髓鞘碱性蛋白(MBP)和Olig-2阳性细胞的面积百分比,从而增强髓鞘化。我们还发现,BMP4、其 mRNA、miR200 的表达与 MBP 和 Olig-2 蛋白的免疫表达呈负相关。这项研究强调,长期服用阿米替林或胆钙化醇后,应激诱导的行为变化会得到改善,miR200/BMP4 信号传导会受到抑制,海马髓鞘化会得到增强,但胆钙化醇似乎对大脑再髓鞘化更有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Amitriptyline and cholecalciferol amend hippocampal histological structure and myelination during stress in Wistar rats via regulating miR200/BMP4/Olig-2 signaling

Chronic stress is a universal condition commonly associated with many psychiatric diseases. An extensive body of evidence discussed hippocampal affection upon chronic stress exposure, however, the underlying molecular pathways still need to be identified. We investigated the impact of chronic stress on miR200/BMP/Olig-2 signaling and hippocampal myelination. We also compared the effects of chronic administration of amitriptyline and cholecalciferol on chronically stressed hippocampi. Both amitriptyline and cholecalciferol significantly decreased serum cortisol levels, reduced immobility time in the forced swim test, increased the number of crossed squares in open field test, decreased the hippocampal expression of bone morphogenetic protein 4 (BMP4) and its messenger RNA (mRNA) levels, reduced miR200 expression as compared to untreated chronically stressed rats. Also, both drugs amended the hippocampal neuronal damage, enhanced the surviving cell count, and increased the pyramidal layer thickness of Cornu Ammonis subregion 1 (CA1) and granule cell layer of the dentate gyrus. Cholecalciferol was more effective in increasing the area percentage of myelin basic protein (MBP) and Olig-2 positive cells count in hippocampi of chronic stress-exposed rats than amitriptyline, thus enhancing myelination. We also found a negative correlation between the expression of BMP4, its mRNA, miR200, and the immunoexpression of MBP and Olig-2 proteins. This work underscores the amelioration of the stress-induced behavioral changes, inhibition of miR200/BMP4 signaling, and enhancement of hippocampal myelination following chronic administration of either amitriptyline or cholecalciferol, though cholecalciferol seemed more effective in brain remyelination.

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来源期刊
Cell Biology International
Cell Biology International 生物-细胞生物学
CiteScore
7.60
自引率
0.00%
发文量
208
审稿时长
1 months
期刊介绍: Each month, the journal publishes easy-to-assimilate, up-to-the minute reports of experimental findings by researchers using a wide range of the latest techniques. Promoting the aims of cell biologists worldwide, papers reporting on structure and function - especially where they relate to the physiology of the whole cell - are strongly encouraged. Molecular biology is welcome, as long as articles report findings that are seen in the wider context of cell biology. In covering all areas of the cell, the journal is both appealing and accessible to a broad audience. Authors whose papers do not appeal to cell biologists in general because their topic is too specialized (e.g. infectious microbes, protozoology) are recommended to send them to more relevant journals. Papers reporting whole animal studies or work more suited to a medical journal, e.g. histopathological studies or clinical immunology, are unlikely to be accepted, unless they are fully focused on some important cellular aspect. These last remarks extend particularly to papers on cancer. Unless firmly based on some deeper cellular or molecular biological principle, papers that are highly specialized in this field, with limited appeal to cell biologists at large, should be directed towards journals devoted to cancer, there being very many from which to choose.
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