Meng Zhang, Xiuli Zheng, Chunyan Wang, Shengmian Li
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引用次数: 0
摘要
理由：本研究采用基于蛋白质组学的数据独立获取（DIA）技术，旨在筛选I型胃神经内分泌肿瘤（g-NEN）中的差异表达蛋白：方法：利用液相色谱/串联质谱（DIA-LC/MS/MS）技术筛选 I 型胃神经内分泌瘤和瘤周组织中的差异表达蛋白。然后使用生物信息学方法对鉴定出的蛋白质进行功能分析。我们选择了三种表达量最高的蛋白质，结合患者的临床数据进行预后分析：结果：与瘤周组织相比，224个蛋白上调，70个蛋白下调。最明显富集的生物过程和通路是空泡质子转运V型ATP酶复合物组装和代谢相关通路。在I型g-NENs中，PCSK1、FBXO2、ACSL1、IRS2和PTPRZ1的表达明显上调。IRS2的高表达与较短的复发时间明显相关：我们的研究提供了基于 DIA-LC/MS/MS 的全面蛋白质组特征，并强调 IRS2 高表达是 I 型 g-NENs 的潜在预后标志物。
Data-independent acquisition combined with liquid chromatography mass spectrometry technique to detect prognostic protein markers in type I gastric neuroendocrine neoplasm
Rationale
This study used proteomics-based data-independent acquisition (DIA) technology with the aim of screening for differential expression proteins in type I gastric neuroendocrine neoplasm (g-NEN).
Methods
Differential expression proteins in type I g-NEN and peritumoral tissues were screened using DIA with liquid chromatography/tandem mass spectrometry (DIA-LC/MS/MS). The identified proteins were then functionally analysed using bioinformatics methods. We selected the three most highly expressed proteins, combined with patients' clinical data, for prognostic analysis.
Results
Compared with peritumoral tissues, 224 proteins were up-regulated, and 70 were down-regulated. The most significantly enriched biological processes and pathways were vacuolar proton-transporting V-type ATPase complex assembly and metabolism-related pathways. PCSK1, FBXO2, ACSL1, IRS2, and PTPRZ1 expression was markedly up-regulated in type I g-NENs. High IRS2 expression significantly correlated with a shorter time to recurrence.
Conclusions
Our study provides a comprehensive proteomic signature based on DIA-LC/MS/MS and highlights high IRS2 expression as a potential prognostic marker for type I gNENs.
期刊介绍：
Rapid Communications in Mass Spectrometry is a journal whose aim is the rapid publication of original research results and ideas on all aspects of the science of gas-phase ions; it covers all the associated scientific disciplines. There is no formal limit on paper length ("rapid" is not synonymous with "brief"), but papers should be of a length that is commensurate with the importance and complexity of the results being reported. Contributions may be theoretical or practical in nature; they may deal with methods, techniques and applications, or with the interpretation of results; they may cover any area in science that depends directly on measurements made upon gaseous ions or that is associated with such measurements.
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