使用混合草药提取物配制漱口水,以控制主要口腔病原体和生物膜

IF 2.2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
A. Sakthivel , K. Sankaran , G. Rengasamy , V. Vishnu Priya , P. Sathishkumar
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引用次数: 0

摘要

导言在全球范围内,口腔疾病是影响生活质量的主要健康问题。口腔微生物菌群在口腔疾病中的作用已得到公认。然而,随着口腔疾病发病率的增加,现有药物对抗菌药物产生了抗药性,因此人们不得不从植物中寻找替代药物。方法通过抑菌区试验评估植物提取物对口腔病原体的抗菌活性。漱口水是用每种植物提取物(大马士革蔷薇花瓣、鹅掌楸叶、番石榴叶和酸浆果)各一克在 10%乙醇中配制而成。通过标准方案评估了配制漱口水对变异链球菌、粪肠球菌、金黄色葡萄球菌、大肠杆菌和白色念珠菌的抗菌功效。此外,还评估了配制漱口水对已形成的变异球菌生物膜的抗生物膜效果,并使用扫描电镜和扫描电子显微镜进行了观察。最低抑菌浓度结果表明,病原体的敏感性如下:金黄色葡萄球菌、变异金黄色葡萄球菌、白喉杆菌、粪大肠杆菌和大肠杆菌。此外,它还具有很好的消炎效果。SEM 和 CLSM 图像显示,配制的漱口水具有抑制金黄色葡萄球菌口腔生物膜的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Formulation of Mouthwash Using Combined Herbal Extracts to Control the Predominant Oral Pathogens and Biofilm

Formulation of Mouthwash Using Combined Herbal Extracts to Control the Predominant Oral Pathogens and Biofilm

Introduction

Globally, oral disease is a major health problem, which affects the quality of life. The role of oral microflora in the oral diseases is well established. However, with the increase in the frequency of oral disease, the development of antimicrobial drug resistance to the existing drugs has insisted on searching for alternative drugs from plant sources. Herein, herbal-based mouthwash is formulated to effectively control the dominant oral pathogens.

Methods

The antimicrobial activity of plant extracts was evaluated against oral pathogens by zone of inhibition test. Mouthwash was formulated with one gram of each plant extracts (Rosa damascena flower petals, Coleus amboinicus leaves, Psidium guajava leaves, and Phyllanthus acidus fruits) in 10% ethanol. Antimicrobial efficacy of the formulated mouthwash was assessed against Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, and Candida albicans by standard protocol. Also, the antibiofilm effect of the formulated mouthwash was assessed against preformed S mutans biofilm and visualised using SEM and CLSM.

Results

The formulated mouthwash exhibits excellent broad-spectrum antimicrobial activity. The minimum inhibitory concentration result indicates that the susceptibility of the pathogens was found as follows: S aureus < S mutans < C albicans < E faecalis < E coli. Also, it shows a great anti-inflammatory effect. SEM and CLSM images reveal that the formulated mouthwash has the potential to inhibit the oral biofilm of S. mutants.

Conclusions

This study concludes that the formulated herbal-based mouthwash might be useful in controlling multiple oral pathogens and biofilms.

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来源期刊
Journal of Herbal Medicine
Journal of Herbal Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
3.90
自引率
0.00%
发文量
94
期刊介绍: The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.
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