核磁共振光谱法作为无色团生物聚合物的替代分析方法:以膳食补充剂中的透明质酸为例

IF 1.1 4区 物理与天体物理 Q4 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL
Fabienne M. Lang, Klaudia Adels, Bernd W. K. Diehl, Margit Schulze, Yulia B. Monakhova
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引用次数: 0

摘要

为满足市场对用于治疗骨关节炎或润肤的膳食补充剂(DSs)和营养化妆品中透明质酸(HA)日益增长的需求,必须有一种准确可靠的方法来分析最终产品中的透明质酸。该研究旨在开发和验证替代方法,分别使用 80 MHz 和 600 MHz 的低场(LF)和高场(HF)核磁共振(NMR)光谱对 DS 中的 HA 进行质量控制。此外,还可同时对 DS 中的另一种活性成分硫酸软骨素(CH)进行定量。1H-NMR 方法的检出限(LOD)和定量限(LOQ)分别为 0.1 毫克/毫升和 0.2 毫克/毫升(80 MHz)以及 0.2 毫克/毫升和 0.6 毫克/毫升(600 MHz)。两种光谱仪的回收率估计在 92% 和 120% 之间;600 MHz 和 80 MHz 的精确度(包括样品制备)分别为 4.2% 和 8.0%。对于基质不同的 16 种 DS,高频和低频 NMR 获得的定量结果具有可比性。70 ℃ 下的高频 NMR 实验是对多组分 DS 中的 HA 和 CH 进行质量控制的简单而有效的工具。在酸水解之前进行的台式 NMR 测量为分析不含 CH 和顺磁基质成分的 DS 提供了一种经济、无低温的替代方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

NMR Spectroscopy as an Alternative Analytical Method for Biopolymers Without Chromophore: Example of Hyaluronic Acid in Dietary Supplements

NMR Spectroscopy as an Alternative Analytical Method for Biopolymers Without Chromophore: Example of Hyaluronic Acid in Dietary Supplements

To respond to the increasing demand for hyaluronic acid (HA) in dietary supplements (DSs) and nutricosmetics marketed for the treatment of osteoarthritis or moistening, it is essential to have an accurate and reliable method for its analysis in the final products. The study aimed to develop and validate alternative method for the quality control of HA in DSs using low-field (LF) and high-field (HF) nuclear magnetic resonance (NMR) spectroscopy at 80 MHz and 600 MHz, respectively. Moreover, chondroitin sulphate (CH), another active ingredient in DSs, can be simultaneously quantified. The 1H-NMR methods have been successfully validated in terms of limit of detection (LOD) and limit of quantitation (LOQ), which were found to be 0.1 mg/mL and 0.2 mg/mL (80 MHz) as well as 0.2 mg/mL and 0.6 mg/mL (600 MHz). Recovery rates were estimated to be between 92 and 120% on both spectrometers; precision including sample preparation was found to be 4.2% and 8.0% for 600 MHz and 80 MHz, respectively. Quantitative results obtained by HF and LF NMR were comparable for 16 DSs with varying matrix. HF NMR experiments at 70 ℃ serve as a simple and efficient quality control tool for HA and CH in multicomponent DSs. Benchtop NMR measurements, upon preceding acid hydrolysis, offer a cost-effective and cryogen-free alternative for analyzing DSs in the absence of CH and paramagnetic matrix components.

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来源期刊
Applied Magnetic Resonance
Applied Magnetic Resonance 物理-光谱学
CiteScore
1.90
自引率
10.00%
发文量
59
审稿时长
2.3 months
期刊介绍: Applied Magnetic Resonance provides an international forum for the application of magnetic resonance in physics, chemistry, biology, medicine, geochemistry, ecology, engineering, and related fields. The contents include articles with a strong emphasis on new applications, and on new experimental methods. Additional features include book reviews and Letters to the Editor.
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