在微流体平台上设计用于细胞排列的独立电纺 PLLCL 纤维

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Özüm Yildirim-Semerci, Ahu Arslan-Yildiz
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引用次数: 0

摘要

本文通过在聚甲基丙烯酸甲酯(PMMA)微流体芯片上直接电纺聚(L-内酯-共ε-己内酯)(PLLCL),开发了一种 PLLCL 芯片平台。设计的微芯片可以电纺独立排列的 PLLCL 纤维,从而消除了传统电纺的局限性。此外,排列整齐的纤维结构有利于通过非接触式操作使细胞排列整齐。通过扫描电子显微镜分析评估了纤维的平均直径和纤维排列,然后研究了微芯片的泄漏曲线。使用 HeLa 和 NIH-3T3 细胞进行了三维细胞培养研究,结果表明,在 PLLCL 芯片上培养 15 天后,细胞存活率接近 85%,而根据活死细胞和阿拉玛蓝分析,二维对照组的细胞存活率在 7 天后开始下降。这些发现强调了用于三维细胞培养的片上 PLLCL 平台的生物相容性及其模拟细胞外基质(ECM)的能力。免疫染色结果证明,与对照组相比,芯片上 PLLCL 平台有利于 ECM 蛋白的分泌,而且芯片上 PLLCL 平台的细胞骨架排列整齐,而对照组的细胞骨架排列无序。总之,这些结果表明所开发的平台适用于形成各种三维细胞培养模型,是细胞排列研究的潜在候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment

Engineering free-standing electrospun PLLCL fibers on microfluidic platform for cell alignment

Here, a PLLCL-on-chip platform was developed by direct electrospinning of poly (L-lactide-co-ε-caprolactone) (PLLCL) on polymethyl methacrylate (PMMA) microfluidic chips. Designed microchip provides the electrospinning of free-standing aligned PLLCL fibers which eliminates limitations of conventional electrospinning. Besides, aligned fiber structure favors cell alignment through contactless manipulation. Average fiber diameter, and fiber alignment was evaluated by SEM analyses, then, leakage profile of microchip was investigated. 3D cell culture studies were conducted using HeLa and NIH-3T3 cells, and nearly 85% cell viability was observed in PLLCL-on-chip for 15 days, while cell viability of 2D control started to decrease after 7 days based on Live dead and Alamar Blue analyses. These findings emphasize biocompatibility of PLLCL-on-chip platform for 3D cell culture and its ability to mimic extracellular matrix (ECM). Immunostaining results prove that PLLCL-on-chip platform favors the secretion of ECM proteins compared to control groups, and cytoskeletons of cells were in aligned orientation in PLLCL-on-chip, while they were in random orientation in control groups. Overall, these results demonstrate that the developed platform is suitable for the formation of various 3D cell culture models and a potential candidate for cell alignment studies.

Graphical Abstract

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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