大鼠模型中 "牵引性阴道生成 "的组织学分析

IF 2.7 Q2 PATHOLOGY
Hannah Meyer, Lexus Trosclair, Sean D. Clayton, Collyn C. O’Quin, Carol Crochet, Joshua C. Colvin, Valerie L Welch, Ahmed Alhaque, Giovanni Solitro, Mila Shah-Bruce, J. Alexander, Donald Sorrells
{"title":"大鼠模型中 \"牵引性阴道生成 \"的组织学分析","authors":"Hannah Meyer, Lexus Trosclair, Sean D. Clayton, Collyn C. O’Quin, Carol Crochet, Joshua C. Colvin, Valerie L Welch, Ahmed Alhaque, Giovanni Solitro, Mila Shah-Bruce, J. Alexander, Donald Sorrells","doi":"10.3390/pathophysiology31020022","DOIUrl":null,"url":null,"abstract":"Vaginal agenesis (VA) is frequently associated with mullerian agenesis. VA treatments include mechanical dilation and surgical vaginoplasty. We created a vaginal expansion sleeve (VES) as a novel device to progressively lengthen the vaginal canal. This study evaluated the histologic effects of the VES on rat vaginal tissue. The VES is a spring-like device made of proprietary woven cylindrical material and flat resin caps. The VESs were constructed as 25–30 mm, pre-contracted springs, which were secured into the vaginas of six Sprague Dawley rats and allowed to re-expand post-surgically. After one week, the VESs were removed, and the vaginas were harvested and measured in length. Test (n = 6) and control (n = 4) formalin-fixed paraffin-embedded tissues were stained with hematoxylin and eosin (H&E), Masson’s trichrome, and anti-Desmin antibodies. The VESs achieved significant vaginal lengthening. The mean vaginal canal length increased from 20.0 ± 2.4 mm to 23.8 ± 1.2 mm after removal of the VESs (n = 6, p < 0.001), a 19% increase. There was a positive correlation between the expander/tension generated in the vagina and the amount of acute and chronic inflammation. H&E staining revealed increased submucosal eosinophilia in five of the six test tissues. One VES sample that was lengthened to 30 mm long showed evidence of lymphocytic and neutrophilic inflammation. Desmin immunostaining and Masson’s trichrome stain revealed a thinner muscularis with more infiltrative fibrous tissue between muscle fibers in the test tissue compared to the control tissue. Although effective, the VES may provoke at least a transient increase in eosinophils consistent with a localized immune reaction during muscularis remodeling.","PeriodicalId":19852,"journal":{"name":"Pathophysiology","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Histologic Analysis of ‘Distraction Vaginogenesis’ in a Rat Model\",\"authors\":\"Hannah Meyer, Lexus Trosclair, Sean D. Clayton, Collyn C. O’Quin, Carol Crochet, Joshua C. Colvin, Valerie L Welch, Ahmed Alhaque, Giovanni Solitro, Mila Shah-Bruce, J. Alexander, Donald Sorrells\",\"doi\":\"10.3390/pathophysiology31020022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Vaginal agenesis (VA) is frequently associated with mullerian agenesis. VA treatments include mechanical dilation and surgical vaginoplasty. We created a vaginal expansion sleeve (VES) as a novel device to progressively lengthen the vaginal canal. This study evaluated the histologic effects of the VES on rat vaginal tissue. The VES is a spring-like device made of proprietary woven cylindrical material and flat resin caps. The VESs were constructed as 25–30 mm, pre-contracted springs, which were secured into the vaginas of six Sprague Dawley rats and allowed to re-expand post-surgically. After one week, the VESs were removed, and the vaginas were harvested and measured in length. Test (n = 6) and control (n = 4) formalin-fixed paraffin-embedded tissues were stained with hematoxylin and eosin (H&E), Masson’s trichrome, and anti-Desmin antibodies. The VESs achieved significant vaginal lengthening. The mean vaginal canal length increased from 20.0 ± 2.4 mm to 23.8 ± 1.2 mm after removal of the VESs (n = 6, p < 0.001), a 19% increase. There was a positive correlation between the expander/tension generated in the vagina and the amount of acute and chronic inflammation. H&E staining revealed increased submucosal eosinophilia in five of the six test tissues. One VES sample that was lengthened to 30 mm long showed evidence of lymphocytic and neutrophilic inflammation. Desmin immunostaining and Masson’s trichrome stain revealed a thinner muscularis with more infiltrative fibrous tissue between muscle fibers in the test tissue compared to the control tissue. Although effective, the VES may provoke at least a transient increase in eosinophils consistent with a localized immune reaction during muscularis remodeling.\",\"PeriodicalId\":19852,\"journal\":{\"name\":\"Pathophysiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-06-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathophysiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3390/pathophysiology31020022\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PATHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathophysiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/pathophysiology31020022","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PATHOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

阴道发育不良(VA)经常与穆勒氏管发育不良有关。治疗阴道缺失的方法包括机械扩张术和阴道成形术。我们创造了一种阴道扩张套(VES),作为逐步延长阴道管的新型装置。本研究评估了 VES 对大鼠阴道组织的组织学影响。VES 是一种类似弹簧的装置,由专有的圆柱形编织材料和扁平树脂帽制成。VES 被制成 25-30 毫米的预收缩弹簧,将其固定在六只 Sprague Dawley 大鼠的阴道内,并让其在手术后重新膨胀。一周后,取出 VES,收获阴道并测量其长度。用苏木精和伊红(H&E)、Masson 三色和抗 Desmin 抗体对试验组织(n = 6)和对照组织(n = 4)进行福尔马林固定石蜡包埋染色。VES 明显延长了阴道长度。移除 VES 后,平均阴道长度从 20.0 ± 2.4 mm 增加到 23.8 ± 1.2 mm(n = 6,p < 0.001),增加了 19%。阴道内产生的扩张器/张力与急性和慢性炎症的数量呈正相关。H&E 染色显示,在六个测试组织中,有五个组织的粘膜下嗜酸性粒细胞增多。一个被延长至 30 毫米长的 VES 样本显示出淋巴细胞和中性粒细胞炎症的证据。Desmin 免疫染色法和 Masson 三色染色法显示,与对照组织相比,测试组织的肌肉组织更薄,肌纤维之间的浸润性纤维组织更多。VES 虽然有效,但至少会引起嗜酸性粒细胞的短暂增加,这与肌肉重塑过程中的局部免疫反应一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histologic Analysis of ‘Distraction Vaginogenesis’ in a Rat Model
Vaginal agenesis (VA) is frequently associated with mullerian agenesis. VA treatments include mechanical dilation and surgical vaginoplasty. We created a vaginal expansion sleeve (VES) as a novel device to progressively lengthen the vaginal canal. This study evaluated the histologic effects of the VES on rat vaginal tissue. The VES is a spring-like device made of proprietary woven cylindrical material and flat resin caps. The VESs were constructed as 25–30 mm, pre-contracted springs, which were secured into the vaginas of six Sprague Dawley rats and allowed to re-expand post-surgically. After one week, the VESs were removed, and the vaginas were harvested and measured in length. Test (n = 6) and control (n = 4) formalin-fixed paraffin-embedded tissues were stained with hematoxylin and eosin (H&E), Masson’s trichrome, and anti-Desmin antibodies. The VESs achieved significant vaginal lengthening. The mean vaginal canal length increased from 20.0 ± 2.4 mm to 23.8 ± 1.2 mm after removal of the VESs (n = 6, p < 0.001), a 19% increase. There was a positive correlation between the expander/tension generated in the vagina and the amount of acute and chronic inflammation. H&E staining revealed increased submucosal eosinophilia in five of the six test tissues. One VES sample that was lengthened to 30 mm long showed evidence of lymphocytic and neutrophilic inflammation. Desmin immunostaining and Masson’s trichrome stain revealed a thinner muscularis with more infiltrative fibrous tissue between muscle fibers in the test tissue compared to the control tissue. Although effective, the VES may provoke at least a transient increase in eosinophils consistent with a localized immune reaction during muscularis remodeling.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Pathophysiology
Pathophysiology Medicine-Pathology and Forensic Medicine
CiteScore
3.10
自引率
0.00%
发文量
48
期刊介绍: Pathophysiology is an international journal which publishes papers in English which address the etiology, development, and elimination of pathological processes. Contributions on the basic mechanisms underlying these processes, model systems and interdisciplinary approaches are strongly encouraged.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信