K. Hosseini, T. Zivari-Ghader, P. Akbarzadehlaleh, V. Ebrahimi, B. E. Sharafabad, A. Dilmaghani
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A Comprehensive Review of L-Asparaginase: Production, Applications and Therapeutic Potential in Cancer Treatment
L-Asparaginase (EC 3.5.1.1) primarily catalyzes the hydrolysis of Asn into ammonium and L-Asp and has wide applications in various industrial sectors. This protein has several sources, including microorganisms, plants, and the serum of certain rodents. Currently, L-asparaginase is primarily produced through biotechnology using bacteria and fungi. Genetic engineering methods are employed to create more stable enzymes than those available for the wild type. Given the extensive industrial use of L-asparaginase, optimization, and purification techniques are utilized to increase ex vivo enzyme production. The objective of this study is to comprehensively review the current literature on the production of L-asparaginase from diverse sources and using various methodologies.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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