利用傅立叶变换红外光谱进行微生物菌种分类的综合方法

IF 2.3 Q3 BIOCHEMICAL RESEARCH METHODS
F. Muchaamba, Roger Stephan
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引用次数: 0

摘要

及时准确地检测微生物威胁并确定其特征对于有效管理感染和疫情爆发至关重要。此外,在食品生产中,快速鉴定微生物对于保持质量控制和卫生标准也是不可或缺的。目前对微生物菌株进行分型的方法通常依赖于劳动密集型、耗时且昂贵的 DNA 和血清分型技术,这限制了它们在快速反应场景中的适用性。在这种情况下,利用傅立叶变换红外(FTIR)光谱的 IR Biotyper® 提供了一种新方法,可在 3 小时内为快速菌株分型提供特异性光谱。它包含有关样品制备、数据采集和分析技术的详细指南,可确保生成可靠、可重复的结果。我们强调了 IR Biotyper® 快速准确的分辨能力,展示了其在病原体实时监测和来源追踪方面的潜力,以提高公共卫生和食品安全。我们建议将其整合为一种早期筛选方法,然后利用全基因组测序进行更详细的分析,以优化微生物监测系统的检测准确性和响应效率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Comprehensive Methodology for Microbial Strain Typing Using Fourier-Transform Infrared Spectroscopy
Timely and accurate detection and characterization of microbial threats is crucial for effective infection and outbreak management. Additionally, in food production, rapid microbe identification is indispensable for maintaining quality control and hygiene standards. Current methods for typing microbial strains often rely on labor-intensive, time-consuming, and expensive DNA- and sera-serotyping techniques, limiting their applicability in rapid-response scenarios. In this context, the IR Biotyper®, utilizing Fourier-transform infrared (FTIR) spectroscopy, offers a novel approach, providing specific spectra for fast strain typing within 3 h. This methodology article serves as a comprehensive resource for researchers and technicians aiming to utilize FTIR spectroscopy for microbial strain typing. It encompasses detailed guidelines on sample preparation, data acquisition, and analysis techniques, ensuring the generation of reliable and reproducible results. We highlight the IR Biotyper®’s rapid and accurate discrimination capabilities, showcasing its potential for real-time pathogen monitoring and source-tracking to enhance public health and food safety. We propose its integration as an early screening method, followed by more detailed analysis with whole-genome sequencing, to optimize detection accuracy and response efficiency in microbial surveillance systems.
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来源期刊
Methods and Protocols
Methods and Protocols Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (miscellaneous)
CiteScore
3.60
自引率
0.00%
发文量
85
审稿时长
8 weeks
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