微胶囊冻干益生菌 Levilactobacillus brevis 菌株中的 S 层蛋白质

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-06-11 DOI:10.15567/mljekarstvo.2024.0304

Nina Čuljak

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引用次数: 0

摘要

表面（S）层蛋白的存在是乳酸菌的一种罕见特性，在表达生产菌株的益生特性和保护细胞免受不利环境条件影响方面发挥着重要作用。因此，这项工作的目的是评估之前从人奶中分离出来的四种左旋乳杆菌 MB1、MB2、MB13 和 MB20 菌株的 S 层蛋白的作用。研究结果表明，在模拟胃肠道（GIT）条件下，当 S 蛋白存在于受检的益生菌左旋乳杆菌菌株表面时，其存活率更高，与 Caco-2 细胞的粘附性更好。游离细胞和海藻酸盐微囊化细胞，无论是否含有半乳寡糖（GOS）和果寡糖（FOS），在冷冻干燥和 4 °C 下储存一个月期间，以及在模拟胃肠道条件下（>108 CFU/g），都有很高的存活率，从而满足了选择益生菌株的主要技术标准。与游离细胞相比，封装细胞的冻干存活率更高，这可能是由于海藻酸盐、GOS 和 FOS 作为益生元剂的保护作用。不过，在海藻酸盐中微胶囊化的保加利亚左旋乳杆菌 MB1、MB2、MB13 和 MB20 细胞在储存期间以及在胃肠道和冷冻干燥的苛刻条件下保持存活率方面，GOS 比 FOS 更有保护作用。

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S-layer proteins in microencapsulated freeze-dried probiotic Levilactobacillus brevis strains

The presence of surface (S)-layer proteins as a rare property of lactic acid bacteria plays an important role in the expression of the probiotic properties of the producer strain and protects the cells from unfavourable environmental conditions. Therefore, the aim of this work was to evaluate the role of S-layer proteins of four Levilactobacillus brevis MB1, MB2, MB13, and MB20 strains, previously isolated from human milk. The results showed better survival under simulated gastrointestinal tract (GIT) conditions and better adhesion to Caco-2 cells, achieved when S-proteins were present on the surface of the examined probiotic Levilactobacillus brevis strains. Free cells and cells microencapsulated in alginate, with or without galactooligosaccharides (GOS) and fructooligosaccharides (FOS), survived in high numbers during freeze-drying and one-month storage at 4 °C, as well as during exposure to simulated GIT conditions (>108 CFU/g), thus fulfilling the primary technological criterion for the selection of probiotic strains. Higher survival of freeze-drying of encapsulated cells than free cells, can be attributed to the protective effect of alginate, GOS and FOS as prebiotic agents. However, GOS was more protective than FOS in maintaining the viability of microencapsulated Levilactobacillus brevis MB1, MB2, MB13 and MB20 cells in alginate during storage and under the harsh conditions of GIT and freeze-drying.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.