利用伽马射线合成的聚(N-乙烯基吡咯烷酮/壳聚糖/银纳米颗粒)纳米复合材料的抗癌效果

IF 3.6 4区材料科学 Q2 MATERIALS SCIENCE, COMPOSITES

Journal of Thermoplastic Composite Materials Pub Date : 2024-06-12 DOI:10.1177/08927057241259757

Mohamed Ali Hassan, Mohamed S El-Deap, Ahmed Mahmoud Farag, Ibrahim Y. Abdelrahman, A. M. Abdel-Ghaffar

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引用次数: 0

摘要

利用伽马射线制备了聚(N-乙烯基吡咯烷酮/壳聚糖/银纳米粒子)聚(NVP/CS/AgNPs)纳米复合材料。利用伽马射线将硝酸银还原成 AgNPs，从而在纳米复合材料网络中形成高度稳定和均匀分散的 AgNPs。傅立叶变换红外光谱（FTIR）、X 射线衍射（XRD）、透射电子显微镜（TEM）和扫描电子显微镜与能量色散 X 射线光谱（SEM-EDX）分析用于确定制备的纳米复合材料的特性。利用伽马射线制备的新型生物相容性聚（NVP/CS/AgNPs）纳米复合材料被用作抗癌药物的模型。在对癌细胞的应用方面，研究结果表明，使用浓度为 30 µg/ml 的 Poly (NVP/CS/AgNPs) 纳米复合材料，72 小时后可显著降低 HepG2 细胞的存活率，从 100% 降至 1.5%，72 小时后的 IC50 值为 10.024 µg/ml。此外，在 24、48 和 72 小时的不同时间间隔内，使用 30 µg/ml 和 3.75 µg/ml 不同浓度的 Poly（NVP/CS/AgNPs）纳米复合材料处理癌细胞可增加其细胞毒性。与未处理的细胞相比，处理过的细胞中 MDA 和 H2O2 的变化百分比分别增加了 127% 和 69%，但 CAT 活性降低了 47%。对照组和处理组都进行了相对基因表达。基因表达显示，作为抗凋亡基因的 Bcl-2 基因表达在处理组中下调。与此相反，凋亡基因 BAX 在治疗组中上调，达到 7383 倍。Bax/ Bcl2 的比值达到了 625.19，这是一个很好的凋亡指标。利用 Western 印迹技术，Caspse-3 和 P53 的蛋白表达量分别增加了 203% 和 109%。总之，Poly（NVP/CS/AgNPs）纳米复合材料具有杀死癌细胞的强大功效。

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Anticancer effect of Poly(N-vinyl pyrrolidone/chitosan/silver nanoparticles) nanocomposite synthesized by gamma radiation

Poly(N-vinyl pyrrolidone/chitosan/Ag nanoparticles) Poly (NVP/CS/AgNPs) nanocomposite was prepared by using gamma radiation. Silver nitrate was reduced to AgNPs by using gamma radiation to create highly stable, and evenly dispersed AgNPs within the nanocomposite network. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) analysis are used to identify the characteristics of the prepared nanocomposite. The newly biocompatible Poly (NVP/CS/AgNPs) nanocomposite prepared by the gamma radiation is used as a model for an anticancer drug. Regarding its application on cancer cells, the results of the outcome demonstrate that the Poly (NVP/CS/AgNPs) nanocomposite significantly decreased the HepG2 cell viability from 100% to 1.5% after 72 hours using the concentration of 30 µg/ml, and the IC50 recorded 10.024 µg/ml at 72 hours of treatment. Moreover, the treatment with the Poly (NVP/CS/AgNPs) nanocomposite increases the cytotoxicity against cancer cells at different concentrations 30 µg/ml and 3.75 µg/ml at different time intervals of 24, 48, and 72 hours. The percent change of MDA, and H2O2 increased in treated cells, and was recorded at 127%, and 69%, respectively, but the CAT activity decreased by 47% in comparison with untreated cells. Relative gene expression was performed in the control, and treated group. The gene expression revealed that the Bcl-2 gene expression as the anti-apoptotic gene was downregulated in the treated group. In contrast, the apoptotic gene BAX was upregulated, and reached 7383-fold in the group treated. The Bax/ Bcl2 ratio reached 625.19 which is an excellent indicator of apoptosis. Using western blot, the protein expression increased for Caspse-3 and P53 recorded at 203% and 109%, respectively. In conclusion, Poly (NVP/CS/AgNPs) nanocomposite has a potent effect on killing cancer cells.

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来源期刊

Journal of Thermoplastic Composite Materials 工程技术-材料科学：复合

CiteScore

8.00

自引率

18.20%

发文量

104

审稿时长

5.9 months

期刊介绍： The Journal of Thermoplastic Composite Materials is a fully peer-reviewed international journal that publishes original research and review articles on polymers, nanocomposites, and particulate-, discontinuous-, and continuous-fiber-reinforced materials in the areas of processing, materials science, mechanics, durability, design, non destructive evaluation and manufacturing science. This journal is a member of the Committee on Publication Ethics (COPE).