利用伽马射线合成的聚(N-乙烯基吡咯烷酮/壳聚糖/银纳米颗粒)纳米复合材料的抗癌效果

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-06-12 DOI:10.1177/08927057241259757

Mohamed Ali Hassan, Mohamed S El-Deap, Ahmed Mahmoud Farag, Ibrahim Y. Abdelrahman, A. M. Abdel-Ghaffar

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引用次数: 0

摘要

利用伽马射线制备了聚(N-乙烯基吡咯烷酮/壳聚糖/银纳米粒子)聚(NVP/CS/AgNPs)纳米复合材料。利用伽马射线将硝酸银还原成 AgNPs，从而在纳米复合材料网络中形成高度稳定和均匀分散的 AgNPs。傅立叶变换红外光谱（FTIR）、X 射线衍射（XRD）、透射电子显微镜（TEM）和扫描电子显微镜与能量色散 X 射线光谱（SEM-EDX）分析用于确定制备的纳米复合材料的特性。利用伽马射线制备的新型生物相容性聚（NVP/CS/AgNPs）纳米复合材料被用作抗癌药物的模型。在对癌细胞的应用方面，研究结果表明，使用浓度为 30 µg/ml 的 Poly (NVP/CS/AgNPs) 纳米复合材料，72 小时后可显著降低 HepG2 细胞的存活率，从 100% 降至 1.5%，72 小时后的 IC50 值为 10.024 µg/ml。此外，在 24、48 和 72 小时的不同时间间隔内，使用 30 µg/ml 和 3.75 µg/ml 不同浓度的 Poly（NVP/CS/AgNPs）纳米复合材料处理癌细胞可增加其细胞毒性。与未处理的细胞相比，处理过的细胞中 MDA 和 H2O2 的变化百分比分别增加了 127% 和 69%，但 CAT 活性降低了 47%。对照组和处理组都进行了相对基因表达。基因表达显示，作为抗凋亡基因的 Bcl-2 基因表达在处理组中下调。与此相反，凋亡基因 BAX 在治疗组中上调，达到 7383 倍。Bax/ Bcl2 的比值达到了 625.19，这是一个很好的凋亡指标。利用 Western 印迹技术，Caspse-3 和 P53 的蛋白表达量分别增加了 203% 和 109%。总之，Poly（NVP/CS/AgNPs）纳米复合材料具有杀死癌细胞的强大功效。

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Anticancer effect of Poly(N-vinyl pyrrolidone/chitosan/silver nanoparticles) nanocomposite synthesized by gamma radiation

Poly(N-vinyl pyrrolidone/chitosan/Ag nanoparticles) Poly (NVP/CS/AgNPs) nanocomposite was prepared by using gamma radiation. Silver nitrate was reduced to AgNPs by using gamma radiation to create highly stable, and evenly dispersed AgNPs within the nanocomposite network. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) analysis are used to identify the characteristics of the prepared nanocomposite. The newly biocompatible Poly (NVP/CS/AgNPs) nanocomposite prepared by the gamma radiation is used as a model for an anticancer drug. Regarding its application on cancer cells, the results of the outcome demonstrate that the Poly (NVP/CS/AgNPs) nanocomposite significantly decreased the HepG2 cell viability from 100% to 1.5% after 72 hours using the concentration of 30 µg/ml, and the IC50 recorded 10.024 µg/ml at 72 hours of treatment. Moreover, the treatment with the Poly (NVP/CS/AgNPs) nanocomposite increases the cytotoxicity against cancer cells at different concentrations 30 µg/ml and 3.75 µg/ml at different time intervals of 24, 48, and 72 hours. The percent change of MDA, and H2O2 increased in treated cells, and was recorded at 127%, and 69%, respectively, but the CAT activity decreased by 47% in comparison with untreated cells. Relative gene expression was performed in the control, and treated group. The gene expression revealed that the Bcl-2 gene expression as the anti-apoptotic gene was downregulated in the treated group. In contrast, the apoptotic gene BAX was upregulated, and reached 7383-fold in the group treated. The Bax/ Bcl2 ratio reached 625.19 which is an excellent indicator of apoptosis. Using western blot, the protein expression increased for Caspse-3 and P53 recorded at 203% and 109%, respectively. In conclusion, Poly (NVP/CS/AgNPs) nanocomposite has a potent effect on killing cancer cells.

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567

期刊介绍： ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/Abstracted： Web of Science SCIE Scopus CAS INSPEC Portico