在羊毛微生物的生物分解作用下,绵羊毛纤维的内部脂质及其脂肪酸组成

V. Tkachuk, P. Stapay, N. Z. ,. Ohorodnyk, N. Motko
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引用次数: 0

摘要

微生物对纤维的破坏是绵羊毛常见的损伤。考虑到内部脂质在羊毛纤维表面形成中的决定性作用,这项工作的目的是研究正常羊毛和受损羊毛的结构和脂质组成。研究对象是阿斯卡尼亚细羊毛品种的母羊。在浓密的营养环境中播种后,对微生物的含量进行了估计。用扫描电子显微镜研究了羊毛纤维的表面,用薄层色谱法研究了纤维初步碱性水解后内部脂质的含量,用气液相色谱法研究了脂肪酸的组成。结果表明,与完整羊毛相比,生物分解羊毛中的细菌含量几乎高出三倍,放线菌和蘑菇的含量也更高。羊毛微生物菌群的活动导致角质层受到破坏。与正常羊毛相比,有缺陷的羊毛内部游离脂质和非酯化脂肪酸的含量增加,而与蛋白质结合的脂质和酯化胆固醇以及神经酰胺的含量减少。受损羊毛蛋白质结合脂质中的 18-甲基二十酸含量降低,表明结构脂质通过 18-甲基二十酸与蛋白质共价连接的硫酯键遭到破坏。关键词:18-甲基二十酸18-甲基二十酸 生物破坏 脂肪酸 内部脂质 微生物 蛋白结合脂质 羊毛纤维
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Internal lipids and their fatty acids composition in a sheep wool fiber under biodestruction with fleece microorganisms
Microbiological destruction of fibers is a common damage to sheep’s wool. Considering the defining role of internal lipids in the formation of wool fibers surface the aim of the work was to study the structure and lipid composition of the normal and damaged wool. The research was carried out on ewes of the Askanian fine-wool breed. The content of microorganisms was estimated after sowing on dense nutrient environments. Wool fibers surface was studied by scanning electron microscopy, the content of internal lipids by thin layer chromatography after preliminary alkaline hydrolysis of the fiber, and fatty acids composition by gas-liquid chromatography. Biodestructed wool was shown to contain almost three times more bacteria, as well as higher levels of actinomycetes and mushrooms compared to intact wool. The violation of the cuticular layer was detected as the result of the fleece microflora activity. In a defective wool the content of the free internal lipids and non-esterified fatty acids was increased while the content of protein-bound lipids and esterified cholesterol as well as of ceramides was decreased as compared to normal wool. The level of 18-methyleicosanoic acid in the protein-bound lipids of damaged wool was decreased, indicating the destruction of the thioester bonds by which structural lipids are covalently linked to proteins through 18-methyleicosanoic acid. Keywords: 18-methyleicosanoic acid, biodestruction, fatty acids, internal lipids, microorganisms, protein-bound lipids, sheep’s wool fiber
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