血清细胞外囊泡衍生的 miR-21-5p 和 miR-26a-5p 作为胃癌的非侵入性诊断潜在生物标记物:初步研究。

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jun-Hong Wang, Zhao-Zhao Bai, Xing-Dong Niu, Cheng-Lou Zhu, Tong Liang, Yong-Li Hu, Zhen-Hua Gao, Ming-Xu Da
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引用次数: 0

摘要

目的:胃癌是全球最常见的恶性肿瘤,也是导致癌症相关死亡的第三大原因,迫切需要早期非侵入性诊断。循环中的细胞外囊泡有可能成为以非侵入性方式进行快速诊断的有前途的生物标志物:利用高通量小 RNA 测序技术,我们分析了健康对照组和胃癌患者血清来源的细胞外囊泡中的小 RNA 群体。我们随机选择了差异表达的微RNA(miRNA),并通过反转录-定量实时聚合酶链反应进行了验证。采用接收者操作特征曲线评估 miRNA 对胃癌的预测价值:结果:本研究发现了 193 个差异表达的 miRNA,其中 152 个上调,41 个显著下调。在不同表达的 miRNA 中,miR-21-5p、miR-26a-5p 和 miR-27a-3p 在胃癌患者血清来源的细胞外囊泡中的表达水平显著升高。miR-21-5p 和 miR-27a-3p 与肿瘤大小密切相关。此外,胃癌患者术后血清中 miR-21-5p 和 miR-26a-5p 的表达水平明显下降:本研究发现了血清 miR-21-5p 和 miR-26a-5p 作为胃癌诊断和预后标志物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Serum extracellular vesicle-derived miR-21-5p and miR-26a-5p as non-invasive diagnostic potential biomarkers for gastric cancer: A preliminary study.

Purpose: Gastric cancer is the most common malignancy worldwide and is the third leading cause of cancer-related deaths, urgently requiring an early and non-invasive diagnosis. Circulating extracellular vesicles may emerge as promising biomarkers for the rapid diagnosis in a non-invasive manner.

Methods: Using high-throughput small RNA sequencing, we profiled the small RNA population of serum-derived extracellular vesicles from healthy controls and gastric cancer patients. Differentially expressed microRNAs (miRNAs) were randomly selected and validated by reverse transcription-quantitative real-time polymerase chain reaction. Receiver operating characteristic curves were employed to assess the predictive value of miRNAs for gastric cancer.

Results: In this study, 193 differentially expressed miRNAs were identified, of which 152 were upregulated and 41 were significantly downregulated. Among the differently expressed miRNA, the expression levels of miR-21-5p, miR-26a-5p, and miR-27a-3p were significantly elevated in serum-derived extracellular vesicles of gastric cancer patients. The miR-21-5p and miR-27a-3p were closely correlated with the tumor size. Moreover, the expression levels of serum miR-21-5p and miR-26a-5p were significantly decreased in gastric cancer patients after surgery.

Conclusions: The present study discovered the potential of serum miR-21-5p and miR-26a-5p as promising candidates for the diagnostic and prognostic markers of gastric cancer.

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来源期刊
International Journal of Biological Markers
International Journal of Biological Markers 医学-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
43
期刊介绍: IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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