3'-UTR微RNA靶区单核苷酸多态性的筛选和分析及其对肺肿瘤发生的影响

IF 1.9 4区医学 Q3 PHARMACOLOGY & PHARMACY

Pharmacogenomics Pub Date : 2024-01-01 Epub Date: 2024-05-30 DOI:10.1080/14622416.2024.2355864

Anmol Bhatia, Atul Kumar Upadhyay, Siddharth Sharma

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引用次数: 0

摘要

目的：本研究旨在鉴定与肺癌相关基因的 miRNA 靶位点中影响较大的单核苷酸多态性 (SNP)。材料与方法：肺癌基因来自 Uniprot KB，miRNA 靶位点 SNP 来自 MirSNP、miRdSNP 和 TargetScan。根据结合影响、小等位基因频率和保守性筛选出 SNPs。分析了健康组织与肺癌组织中具有高影响 SNPs 基因的基因表达情况。此外，还进行了富集、通路和网络分析。结果在肺癌相关基因的 miRNA 靶位点中发现了 19 个高影响 SNPs。这些 SNPs 会影响 miRNA 结合和基因表达。这些基因参与了关键的癌症相关通路。结论已确定的高影响 miRNA 靶点 SNPs 和相关基因为不同人群肺癌患者的病例对照研究提供了一个起点。

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Screening and analysis of single nucleotide polymorphism in the 3'-UTR microRNA target regions and its implications for lung tumorigenesis.

Aim: The study aims to identify high-impact single nucleotide polymorphisms (SNPs) in miRNA target sites of genes associated with lung cancer.Materials & methods: Lung cancer genes were obtained from Uniprot KB. miRNA target site SNPs were mined from MirSNP, miRdSNP and TargetScan. SNPs were shortlisted based on binding impact, minor allele frequency and conservation. Gene expression was analyzed in genes with high-impact SNPs in healthy versus lung cancer tissue. Additionally, enrichment, pathway and network analyzes were performed.Results: 19 high-impact SNPs were identified in miRNA target sites of lung cancer-associated genes. These SNPs affect miRNA binding and gene expression. The genes are involved in key cancer related pathways.Conclusion: The identified high-impact miRNA target site SNPs and associated genes provide a starting point for case-control studies in lung cancer patients in different populations.

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来源期刊

Pharmacogenomics 医学-药学

CiteScore

3.40

自引率

9.50%

发文量

审稿时长

4-8 weeks

期刊介绍： Pharmacogenomics (ISSN 1462-2416) is a peer-reviewed journal presenting reviews and reports by the researchers and decision-makers closely involved in this rapidly developing area. Key objectives are to provide the community with an essential resource for keeping abreast of the latest developments in all areas of this exciting field. Pharmacogenomics is the leading source of commentary and analysis, bringing you the highest quality expert analyses from corporate and academic opinion leaders in the field.