Ayesha Malik, Kiran Afshan, Mohammad K. Okla, Ibrahim A. Saleh, Abdul Razzaq, Munib Hussain, Sabika Firasat, Erinda Lika, Màrius Vicent Fuentes
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引用次数: 0
摘要
本研究通过对分子系统发生学的解释，阐明了Rhipicephalus蜱（Ixodidae）的色谱谱系，从而对其进化轨迹进行了全面的研究。这项研究利用先进的色谱工具探索了动态化学图谱，为了解蜱的进化历史和生态适应性提供了宝贵的信息。Rhipicephalus蜱在绵羊和山羊中的流行率分别为4.5%和3.9%。Rhipicephalus sanguineus（OK642408）和 Rhipicephalus microplus（OK642409）的 ITS2 序列与其他国家的序列形成了一个独特的支系。R. sanguineus（OK560870）的 16S rRNA 序列与热带、温带和东南部三个品系的序列聚类。经 Cox I 基因鉴定的 Rhipicephalus turanicus（OK623472）和 R. microplus（OK623463）与序列形成独立的支系。蜱虫样本的 HPLC 色谱图显示了一系列不同的已鉴定碳氢化合物，说明它们的外骨骼具有复杂的化学成分。这种分析方法提供了对特定碳氢化合物特征的宝贵见解，可潜在应用于物种区分、生态学研究，以及更深入地了解碳氢化合物在蜱虫生理中发挥的功能作用。研究结果揭示了将分子系统学工具与色谱法结合应用的潜力，这不仅能提高我们对蜱进化的认识，还能为Rhipicephalus蜱（Ixodidae）流行地区的疾病控制和管理策略提供信息。研究亮点：利用先进的色谱技术绘制化学图谱。扫描显微镜洞察高分辨率扫描工具，在分子水平上观察蜱的结构和形态特征。分子系统发育数据阐明蜱物种之间的进化关系。
Revealing tick diversity: Chemical profiling and dynamics in scanning microscopy and molecular phylogenetics
This study presents a comprehensive investigation into the evolutionary trajectories of Rhipicephalus ticks (Ixodidae) through the interpretation of molecular phylogenetics, elucidating their chromatographic spectrum. The use of advanced chromatographic tools in this study explored the dynamics chemical profiling, providing valuable insights into the evolutionary history and ecological adaptations. Prevalence of Rhipicephalus ticks was 4.5% in sheep and 3.9% in goats. The ITS2 sequence of the Rhipicephalus sanguineus (OK642408) and Rhipicephalus microplus (OK642409) form a distinct clade with sequences from other countries. The 16S rRNA sequences of R. sanguineus (OK560870) clustered with sequences form three lineages, tropical, temperate, and south-eastern. The Cox I gene-identified Rhipicephalus turanicus (OK623472) and R. microplus (OK623463) form separate clades with sequences. The HPLC chromatogram of tick samples reveals a diverse array of identified hydrocarbons, explained the complex chemical composition of their exoskeletons. This analytical approach provides valuable insights into the specific hydrocarbon profiles, allowing for potential applications in species differentiation, ecological studies, and a deeper understanding of the functional roles played by hydrocarbon compounds in tick physiology. The findings revealed the potential of applying molecular phylogenetics tools with chromatography not only to enhance our understanding of tick evolution but also to inform strategies for disease control and management in regions where Rhipicephalus ticks (Ixodidae) are endemic.
Research Highlights
Chemical mapping utilizing advanced chromatographic techniques.
Scanning microscopic insights high-resolution scanning tool to observe structural and morphological features of ticks at a molecular level.
Molecular phylogeny data elucidate the evolutionary relationships among tick species.
期刊介绍：
Microscopy Research and Technique (MRT) publishes articles on all aspects of advanced microscopy original architecture and methodologies with applications in the biological, clinical, chemical, and materials sciences. Original basic and applied research as well as technical papers dealing with the various subsets of microscopy are encouraged. MRT is the right form for those developing new microscopy methods or using the microscope to answer key questions in basic and applied research.
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