水流:观察小型底栖生物间隙行为的新系统

IF 2.2 4区 生物学 Q2 BIOLOGY
Integrative Organismal Biology Pub Date : 2024-05-25 eCollection Date: 2024-01-01 DOI:10.1093/iob/obae016
W M Ballentine, K M Dorgan
{"title":"水流:观察小型底栖生物间隙行为的新系统","authors":"W M Ballentine, K M Dorgan","doi":"10.1093/iob/obae016","DOIUrl":null,"url":null,"abstract":"<p><p>Meiofauna (benthic invertebrates < 1 mm in size) facilitate sediment biogeochemical cycling, alter sediment microbial community structure, and serve as an important trophic link between benthic micro- and macrofauna, yet the behaviors that mechanistically link individuals to their ecological effects are largely unknown. Meiofauna are small and sediments are opaque, making observing the <i>in situ</i> activities of these animals challenging. We developed the Meioflume, a small, acrylic flow tunnel filled with grains of cryolite, a transparent sand analog, to simulate the <i>in situ</i> conditions experienced by meiofauna in an observable lab environment. The Meioflume has a working area (28.57 mm × 10.16 mm × 1 mm) that is small enough to quickly locate fauna and clearly observe behavior but large enough that animals are not tightly confined. When connected to a syringe press, the Meioflume can produce low velocity flows consistently and evenly across the width of its working area while retaining the contents. To demonstrate its functionality in observing the behavior of meiofauna, we placed individual meiofaunal animals (a protodrilid annelid, a harpacticoid copepod, and a platyhelminth flatworm) in Meioflumes and filmed their behavioral response to a sudden initiation of porewater flow. All animals were clearly visible within the flume and could be observed responding to the onset of flow. The design and construction of the Meioflume make it an accessible, affordable tool for researchers. This experimental system could be modified to address many questions in meiofaunal ecology, such as studying behavior in response to chemical cues, allowing us to observe meiofaunal behaviors to better understand their ecological effects.</p>","PeriodicalId":13666,"journal":{"name":"Integrative Organismal Biology","volume":"6 1","pages":"obae016"},"PeriodicalIF":2.2000,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11177882/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Meioflume: A New System for Observing the Interstitial Behavior of Meiofauna.\",\"authors\":\"W M Ballentine, K M Dorgan\",\"doi\":\"10.1093/iob/obae016\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Meiofauna (benthic invertebrates < 1 mm in size) facilitate sediment biogeochemical cycling, alter sediment microbial community structure, and serve as an important trophic link between benthic micro- and macrofauna, yet the behaviors that mechanistically link individuals to their ecological effects are largely unknown. Meiofauna are small and sediments are opaque, making observing the <i>in situ</i> activities of these animals challenging. We developed the Meioflume, a small, acrylic flow tunnel filled with grains of cryolite, a transparent sand analog, to simulate the <i>in situ</i> conditions experienced by meiofauna in an observable lab environment. The Meioflume has a working area (28.57 mm × 10.16 mm × 1 mm) that is small enough to quickly locate fauna and clearly observe behavior but large enough that animals are not tightly confined. When connected to a syringe press, the Meioflume can produce low velocity flows consistently and evenly across the width of its working area while retaining the contents. To demonstrate its functionality in observing the behavior of meiofauna, we placed individual meiofaunal animals (a protodrilid annelid, a harpacticoid copepod, and a platyhelminth flatworm) in Meioflumes and filmed their behavioral response to a sudden initiation of porewater flow. All animals were clearly visible within the flume and could be observed responding to the onset of flow. The design and construction of the Meioflume make it an accessible, affordable tool for researchers. This experimental system could be modified to address many questions in meiofaunal ecology, such as studying behavior in response to chemical cues, allowing us to observe meiofaunal behaviors to better understand their ecological effects.</p>\",\"PeriodicalId\":13666,\"journal\":{\"name\":\"Integrative Organismal Biology\",\"volume\":\"6 1\",\"pages\":\"obae016\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11177882/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Integrative Organismal Biology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/iob/obae016\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Integrative Organismal Biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/iob/obae016","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

小型底栖动物(底栖无脊椎动物)的原位活动具有挑战性。我们开发了一个小型丙烯酸流动隧道--Meioflume,里面装满了冰晶石颗粒(一种透明的沙子类似物),以模拟水底无脊椎动物在可观察的实验室环境中所经历的原位条件。Meioflume 的工作区域(28.57 毫米 × 10.16 毫米 × 1 毫米)足够小,可以快速定位动物并清楚地观察其行为,但又足够大,不会将动物紧紧束缚住。当连接到注射器压力机时,Meioflume 可以在其工作区域的宽度上持续、均匀地产生低速流动,同时保留内容物。为了证明 Meioflume 在观察小型底栖生物行为方面的功能,我们将单个小型底栖生物(一种原口环带虫、一种桡足类桡足虫和一种扁形动物)放入 Meioflume 中,并拍摄它们对突然启动的孔隙水流的行为反应。所有动物在水槽中都清晰可见,并能观察到它们对水流开始时的反应。Meioflume 的设计和构造使其成为研究人员可以使用且负担得起的工具。这种实验系统可用于解决小型底栖生物生态学中的许多问题,如研究对化学线索的行为反应,使我们能够观察小型底栖生物的行为,从而更好地了解它们对生态的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Meioflume: A New System for Observing the Interstitial Behavior of Meiofauna.

Meiofauna (benthic invertebrates < 1 mm in size) facilitate sediment biogeochemical cycling, alter sediment microbial community structure, and serve as an important trophic link between benthic micro- and macrofauna, yet the behaviors that mechanistically link individuals to their ecological effects are largely unknown. Meiofauna are small and sediments are opaque, making observing the in situ activities of these animals challenging. We developed the Meioflume, a small, acrylic flow tunnel filled with grains of cryolite, a transparent sand analog, to simulate the in situ conditions experienced by meiofauna in an observable lab environment. The Meioflume has a working area (28.57 mm × 10.16 mm × 1 mm) that is small enough to quickly locate fauna and clearly observe behavior but large enough that animals are not tightly confined. When connected to a syringe press, the Meioflume can produce low velocity flows consistently and evenly across the width of its working area while retaining the contents. To demonstrate its functionality in observing the behavior of meiofauna, we placed individual meiofaunal animals (a protodrilid annelid, a harpacticoid copepod, and a platyhelminth flatworm) in Meioflumes and filmed their behavioral response to a sudden initiation of porewater flow. All animals were clearly visible within the flume and could be observed responding to the onset of flow. The design and construction of the Meioflume make it an accessible, affordable tool for researchers. This experimental system could be modified to address many questions in meiofaunal ecology, such as studying behavior in response to chemical cues, allowing us to observe meiofaunal behaviors to better understand their ecological effects.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
3.70
自引率
6.70%
发文量
48
审稿时长
20 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信