Zengxu An, Zihan Yang, Yi Zhou, Shaojie Huo, Siyan Zhang, Dianxing Wu, Xiaoli Shu, Yin Wang
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引用次数: 0
摘要
由于低温会抑制植物生长并降低产量,因此迫切需要鉴定参与调控水稻耐寒性的新基因。耐寒性由多个基因位点控制,涉及复杂的调控网络。在这里,我们发现水稻巨球蛋白相关凝集素(OsJRL)能调节水稻的耐寒性。OsJRL 基因功能缺失会增加冷胁迫下的苯丙氨酸代谢和类黄酮生物合成。在冷胁迫下,OsJRL基因敲除(KO)品系比野生型水稻Nipponbare(NIP)具有更高的苯丙氨酸氨解酶(PAL)活性和更多的类黄酮积累。与 NIP 相比,叶片中活性氧(ROS)水平较低,耐寒性明显增强。相比之下,OsJRL过表达(OE)品系的ROS积累水平较高,耐寒性低于NIP。此外,OsJRL KO 株系在冷胁迫下积累的脱落酸(ABA)和茉莉酸(JA)比 NIP 多。与 NIP 相比,OsJRL OE 株系对 ABA 的敏感性有所提高。我们得出结论:OsJRL通过调节苯丙氨酸代谢和类黄酮生物合成来负向调节水稻的耐寒性。
OsJRL negatively regulates rice cold tolerance via interfering phenylalanine metabolism and flavonoid biosynthesis.
The identification of new genes involved in regulating cold tolerance in rice is urgent because low temperatures repress plant growth and reduce yields. Cold tolerance is controlled by multiple loci and involves a complex regulatory network. Here, we show that rice jacalin-related lectin (OsJRL) modulates cold tolerance in rice. The loss of OsJRL gene functions increased phenylalanine metabolism and flavonoid biosynthesis under cold stress. The OsJRL knock-out (KO) lines had higher phenylalanine ammonia-lyase (PAL) activity and greater flavonoid accumulation than the wild-type rice, Nipponbare (NIP), under cold stress. The leaves had lower levels of reactive oxygen species (ROS) and showed significantly enhanced cold tolerance compared to NIP. In contrast, the OsJRL overexpression (OE) lines had higher levels of ROS accumulation and showed lower cold tolerance than NIP. Additionally, the OsJRL KO lines accumulated more abscisic acid (ABA) and jasmonic acid (JA) under cold stress than NIP. The OsJRL OE lines showed increased sensitivity to ABA compared to NIP. We conclude that OsJRL negatively regulates the cold tolerance of rice via modulation of phenylalanine metabolism and flavonoid biosynthesis.
期刊介绍:
Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.