Ugne Simanaviciute , Harry G. Potter , Reinmar Hager , Jocelyn Glazier , Emma Hodson-Tole , John Gigg , Robyn Grant
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引用次数: 0
摘要
多聚 I:C 大鼠后代被用来研究子宫内暴露于母体免疫激活(MIA)的影响,并被认为是神经发育障碍(NDD)的模型。该模型的行为症状多种多样,会因背景品系的选择和饲养方法等外部因素而变化。测量啮齿动物的胡须运动可对其感官、运动和认知行为进行定量、可靠的测量。本研究调查了暴露于 MIA 的母鼠 50 天大的雄性和雌性后代的胡须运动,并与年龄匹配的对照组(车辆)母鼠的后代进行了比较。大鼠后代在光滑和纹理物体的顺序物体探索任务中使用高速录像机进行拍摄。在雌性后代中发现了多聚 I:C 处理效应,在物体探索过程中胡须平均角位置没有增加,尤其是在光滑物体上,这表明存在注意力缺陷。本文首次证明,在物体探索过程中对胡须进行追踪是一种有用、快速和非侵入性的工具,可用于识别 MIA 引起的 NDD 模型中的治疗效果和性别差异。
Maternal immune activation affects female offspring whisker movements during object exploration in a rat model of neurodevelopmental disorders
Poly I:C rat offspring are used to investigate the effects of in utero exposure to maternal immune activation (MIA) and have been suggested as a model of neurodevelopmental disorders (NDD). The behavioural symptoms of this model are diverse and can vary with external factors, including the choice of background strain and husbandry practices. Measuring whisker movements provides quantitative, robust measurements of sensory, motor and cognitive behaviours in rodents. In this study, whisker movements were investigated in 50-day-old male and female offspring of MIA-exposed rat dams and compared to age-matched offspring of control (vehicle) dams. Rat offspring were filmed using high-speed videography in a sequential object exploration task with smooth and textured objects. Poly I:C treatment effects were found in female offspring that did not increase whisker mean angular position during object exploration, especially for the smooth object, indicating an attentional deficit. Whisker tracking during object exploration is demonstrated here, for the first time, as a useful, quick and non-invasive tool to identify both treatment effects and sex differences in a model of MIA-induced NDDs.