滑膜液能否成为硅藻法医分析的有用基质?

IF 1.5 4区 医学 Q2 MEDICINE, LEGAL
Stefano Tambuzzi MD, Guendalina Gentile BSc, Paolo Bailo MD, Riccardo Primavera MD, Salvatore Andreola MD, Riccardo Zoja MD, PhD
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引用次数: 0

摘要

迄今为止,在溺水法医鉴定中,滑膜液还没有作为一种可能的基质进行有针对性的分析,以寻找硅藻的存在。不过,滑膜液具有独特的生成特点,并能与外部环境隔离,因此适合用于这一目的,这与文献中对玻璃体液的研究结果类似。考虑到这一点,我们在一个特殊病例中对滑膜液进行了分析,该病例在尸检过程中被证实同时存在多发性创伤和溺水的迹象,表明在浸泡过程中滑膜液具有生命力。在对滑膜液样本离心后得到的上清液进行薄涂片后,成功检测到硅藻,这与在其他器官和运河水中发现的硅藻一致。在滑液中检测到硅藻是一个客观的发现,但由于这只是一个试点应用,其推广性受到了限制。不过,如果怀疑是溺水死亡,而且尸体有多个部位被外伤冲破,那么分析滑液中硅藻的技术可能大有可为。因此,应该进一步探索这种技术,以便在这种情况下获得更多的法医证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Could synovial fluid be a useful substrate for the forensic analysis of diatoms?

To date, synovial fluid has not been the subject of targeted analysis as a possible substrate to search for the presence of diatoms in the forensic context of drowning. However, its unique characteristics of production and isolation from the external environment could make it suitable for this purpose, similar to what has already been demonstrated in the literature for vitreous humor. By considering this, synovial fluid was analyzed in a specific case that came to our attention, where the coexisting signs of polytrauma and drowning were documented during autopsy, demonstrating a period of vitality during immersion. After a thin smear of the supernatant was obtained from the centrifugation of the synovial fluid sample, diatoms were successfully detected, consistent with those found in other organs and the water of the canal. The detection of diatoms in the synovial fluid was an objective finding, but its generalizability is limited because this was a pilot application. However, in cases where death by drowning is suspected and the body has multiple areas breached by trauma, the technique of analyzing diatoms in the synovial fluid could have great potential. Therefore, it is appropriate to further explore this technique in order to obtain more forensic evidence in such a setting.

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来源期刊
Journal of forensic sciences
Journal of forensic sciences 医学-医学:法
CiteScore
4.00
自引率
12.50%
发文量
215
审稿时长
2 months
期刊介绍: The Journal of Forensic Sciences (JFS) is the official publication of the American Academy of Forensic Sciences (AAFS). It is devoted to the publication of original investigations, observations, scholarly inquiries and reviews in various branches of the forensic sciences. These include anthropology, criminalistics, digital and multimedia sciences, engineering and applied sciences, pathology/biology, psychiatry and behavioral science, jurisprudence, odontology, questioned documents, and toxicology. Similar submissions dealing with forensic aspects of other sciences and the social sciences are also accepted, as are submissions dealing with scientifically sound emerging science disciplines. The content and/or views expressed in the JFS are not necessarily those of the AAFS, the JFS Editorial Board, the organizations with which authors are affiliated, or the publisher of JFS. All manuscript submissions are double-blind peer-reviewed.
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