Shuai Wang, Jia-Nuo He, Ying-Jie Wang, Wen-Ya Zhao, Qing-Xia Yang, Ya-Na Wang, Yang Zhang, Li-Ping Zhang, Hong-Wei Liu
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引用次数: 0
摘要
与植物相关的内生真菌可能含有尚未发现的生物活性化合物。在标准的实验室条件下,大多数未被发现的化合物没有活性,而在不同的栽培条件下,这些化合物的产生会受到刺激。本研究从山东省济南市泉城公园的鹅掌楸(Koelreuteria paniculata)树皮中分离出六种内生真菌,其中一种被鉴定为 Aureobasidium pullulans 的新亚种,命名为 A. pullulans KB3。此外,研究人员还利用代谢组学工具筛选了适合 A. pullulans KB3 发酵的培养基,结果表明蛋白胨葡萄糖培养基(PDM)更有利于培养 A. pullulans KB3 分离新型化合物。通过利用 PDM 进行放大发酵,从 A. pullulans KB3 中初步分离出了一种多酮化合物 Sphaerolone。此外,还对 A. pullulans KB3 的全基因组 DNA 进行了测序,以促进化合物的分离并确定生物合成基因簇(BGC)。本研究报告了对 A. pullulans KB3 的多组学(代谢组和基因组)分析,为发现沉默 BGCs 的新型化合物并确定其生物合成途径奠定了基础。
Metabolome and Genome Analysis of a Novel Endophytic Fungus Aureobasidium pullulans KB3: Discovery of Polyketones and Polyketone Biosynthesis Pathway.
Endophytic fungi associated with plants may contain undiscovered bioactive compounds. Under standard laboratory conditions, most undiscovered compounds are inactive, whereas their production could be stimulated under different cultivation conditions. In this study, six endophytic fungi were isolated from the bark of Koelreuteria paniculata in Quancheng Park, Jinan City, Shandong Province, one of which was identified as a new subspecies of Aureobasidium pullulans, named A. pullulans KB3. Additionally, metabolomic tools were used to screen suitable media for A. pullulans KB3 fermentation, and the results showed that peptone dextrose medium (PDM) was more beneficial to culture A. pullulans KB3 for isolation of novel compounds. Sphaerolone, a polyketone compound, was initially isolated from A. pullulans KB3 via scaled up fermentation utilizing PDM. Additionally, the whole-genome DNA of A. pullulans KB3 was sequenced to facilitate compound isolation and identify the biosynthesis gene clusters (BGCs). This study reports the multi-omics (metabolome and genome) analysis of A. pullulans KB3, laying the foundation for discovering novel compounds of silent BGCs and identifying their biosynthesis pathway.
期刊介绍:
Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses.
Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication.
Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses.
Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods.
Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.