解密波形蛋白组装：连接理论模型与实验方法

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecules and Cells Pub Date : 2024-07-01 DOI:10.1016/j.mocell.2024.100080

Soyeon Jeong , Nam-Chul Ha

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引用次数: 0

摘要

波形蛋白中间丝（IFs）是真核生物细胞骨架的关键组成部分，其复杂的组装过程尚待阐明。在这项工作中，我们研究了从可溶性四聚体波形蛋白单位到成熟的 11-nm 管状丝的转变过程，解决了人们对波形蛋白中间丝组装理解的一个重大空白。通过理论建模和实验数据分析的结合，我们提出了一种新的组装序列，强调了可溶性四聚体的螺旋转折和间隙填充的作用。我们的发现揭示了波形蛋白独特的结构动力学，并对中间丝形成的一般原理产生了更广泛的影响。

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Deciphering vimentin assembly: Bridging theoretical models and experimental approaches

The intricate assembly process of vimentin intermediate filaments (IFs), key components of the eukaryotic cytoskeleton, has yet to be elucidated. In this work, we investigated the transition from soluble tetrameric vimentin units to mature 11-nm tubular filaments, addressing a significant gap in the understanding of IF assembly. Through a combination of theoretical modeling and analysis of experimental data, we propose a novel assembly sequence, emphasizing the role of helical turns and gap filling by soluble tetramers. Our findings shed light on the unique structural dynamics of vimentin and suggest broader implications for the general principles of IF formation.

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来源期刊

Molecules and Cells 生物-生化与分子生物学

CiteScore

6.60

自引率

10.50%

发文量

审稿时长

2.3 months

期刊介绍： Molecules and Cells is an international on-line open-access journal devoted to the advancement and dissemination of fundamental knowledge in molecular and cellular biology. It was launched in 1990 and ISO abbreviation is ''Mol. Cells''. Reports on a broad range of topics of general interest to molecular and cell biologists are published. It is published on the last day of each month by the Korean Society for Molecular and Cellular Biology.