加强核心针活检的淋巴瘤诊断:免疫组化与流式细胞术的结合。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Silvia Bellesi, Gabriele Schiaffini, Andrea Contegiacomo, Elena Maiolo, Camilla Iacovelli, Rosalia Malafronte, Simone D'Innocenzo, Eleonora Alma, Flaminia Bellisario, Marcello Viscovo, Fabrizia Campana, Alessandra De Filippis, Francesco D'Alò, Luigi Maria Larocca, Valerio De Stefano, Roberto Iezzi, Stefan Hohaus
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引用次数: 0

摘要

图像引导核心针活检(IG-CNB)是一种微创方法,可用于获取淋巴腺病和疑似淋巴瘤患者的组织。尽管这种方法很有用,但诊断方面的挑战依然存在,与切除活检相比疗效较低。我们的研究旨在评估在对疑似淋巴组织增生性疾病进行 IG-CNB 检查时,将流式细胞术(FC)与免疫组化(IHC)结合使用的潜在效用。我们分析了 170 例连续病例,在超声(94 例)或计算机断层扫描(76 例)的引导下,我们采用了一种在实验室实践中已经确立的诊断算法,利用三个配备鸡尾酒抗体的试管来确定淋巴瘤,尤其是 B 细胞来源的淋巴瘤。FC 加快了诊断过程,87.6%的病例在 48 小时内得到推定结果,阳性预测值高达 98%。在常规 IHC 中加入 FC,诊断率从 91.2% 提高到 95.3%,IG-CNB 失败率降低了 45%,从 8.8% 降至 4.7%。这种提高对深部病灶和疑似疾病复发的情况尤为明显。因此,FC 成为一种有价值的辅助工具,可提高诊断效果,尤其是量化靶向治疗表面标志物表达的能力,并有可能减少 IG-CNB 术后重复切除活检的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancing lymphoma diagnosis on core needle biopsies: Integrating immunohistochemistry with flow cytometry.

Image-guided core needle biopsies (IG-CNB) represent a minimally invasive approach for obtaining tissue in patients with lymphadenopathy and suspected lymphoma. Despite their utility, diagnostic challenges persist, with lower efficacy compared with excisional biopsies. Our study aimed to evaluate the potential utility of incorporation of flow cytometry (FC) alongside immunohistochemistry (IHC) when performing IG-CNB for suspected lymphoproliferative diseases. Analyzing 170 consecutive cases, guided by ultrasound (n = 94) or computer tomography (n = 76), we employed a diagnostic algorithm, already established in our laboratory practice, utilizing three antibody cocktail-equipped tubes tailored for defining lymphomas, particularly those of B-cell origin. FC expedited the diagnostic process, yielding presumptive results in 87.6% of cases within 48 h, with a positive predictive value of 98%. Addition of FC to routine IHC enhanced the diagnostic rate from 91.2% to 95.3%, reducing IG-CNB failure rate by 45%, from 8.8% to 4.7%. This enhancement was particularly notable for deep-seated sites and in the setting of suspected disease recurrences. Consequently, FC emerges as a valuable adjunctive tool, allowing for the improvement of diagnostic performance, with a particular focus on the ability to quantify the expression of surface markers for targeted therapies, and holding the potential to diminish the necessity for repeat excisional biopsies subsequent to IG-CNB procedures.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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