小鼠主动脉心脏瓣膜的体内三维几何重建。

IF 3 2区 医学 Q3 ENGINEERING, BIOMEDICAL
Daniel P. Gramling, Aletea L. van Veldhuisen, Frederick W. Damen, Kaitlyn Thatcher, Felix Liu, David McComb, Joy Lincoln, Christopher K. Breuer, Craig J. Goergen, Michael S. Sacks
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引用次数: 0

摘要

在美国,主动脉瓣(AV)疾病是一种常见的瓣膜病变,65 岁时发病率约为 5%,随着年龄的增长,发病率也在不断上升。虽然目前的心脏瓣膜置换术延长了许多人的寿命,但其长期使用仍受到耐用性的限制。目前还没有针对房室疾病的非手术疗法,这在很大程度上是因为我们对房室疾病病因的了解还不全面。对人类房室疾病的直接研究仍然受到以下事实的阻碍:只有在治疗时才能获得临床数据,此时疾病已处于或接近终末期,任何时间进展信息都已丢失。大型动物模型长期以来一直用于评估替代性房室设备,但目前还不能再现房室疾病的过程。作为一种重要的替代方法,小鼠动物模型因其能够对房室疾病过程进行遗传研究和测试潜在的药物治疗而具有吸引力。虽然小鼠模型已被用于房室疾病的细胞和遗传研究,但其较小的体型和较快的心率阻碍了它们用于组织和器官水平的研究。我们最近开发了一种基于微计算机断层扫描的新型体外方法来三维重建小鼠心脏瓣膜并估算瓣叶机械行为(Feng 等,发表于《科学报告》13(1):12852, 2023)。在本研究中,我们利用高频四维超声(4DUS)将我们的方法扩展到体内功能性小鼠房室(mAV)几何形状的三维重建。我们从生成的 4DUS 图像中数字化了完全关闭和完全打开状态下的 mAV 中表面坐标。然后,我们利用匹配的体外小鼠 mAV 高分辨率 µCT 图像建立了基于 NURBS 的 mAV 几何模型。然后,我们将 mAV 几何模型拟合到体内数据,重建了 n = 3 个 mAV 在闭合和打开状态下的三维体内 mAV 几何结构。结果显示了高保真的几何结果。据我们所知,这是首次实现这种重建。这种对体内主动脉瓣叶运动学进行三维稳健评估的方法,为小鼠主动脉瓣疾病模型的纵向特征描述提供了可能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In Vivo Three-Dimensional Geometric Reconstruction of the Mouse Aortic Heart Valve

In Vivo Three-Dimensional Geometric Reconstruction of the Mouse Aortic Heart Valve

Aortic valve (AV) disease is a common valvular lesion in the United States, present in about 5% of the population at age 65 with increasing prevalence with advancing age. While current replacement heart valves have extended life for many, their long-term use remains hampered by limited durability. Non-surgical treatments for AV disease do not yet exist, in large part because our understanding of AV disease etiology remains incomplete. The direct study of human AV disease remains hampered by the fact that clinical data is only available at the time of treatment, where the disease is at or near end stage and any time progression information has been lost. Large animal models, long used to assess replacement AV devices, cannot yet reproduce AV disease processes. As an important alternative mouse animal models are attractive for their ability to perform genetic studies of the AV disease processes and test potential pharmaceutical treatments. While mouse models have been used for cellular and genetic studies of AV disease, their small size and fast heart rates have hindered their use for tissue- and organ-level studies. We have recently developed a novel ex vivo micro-CT-based methodology to 3D reconstruct murine heart valves and estimate the leaflet mechanical behaviors (Feng et al. in Sci Rep 13(1):12852, 2023). In the present study, we extended our approach to 3D reconstruction of the in vivo functional murine AV (mAV) geometry using high-frequency four-dimensional ultrasound (4DUS). From the resulting 4DUS images we digitized the mAV mid-surface coordinates in the fully closed and fully opened states. We then utilized matched high-resolution µCT images of ex vivo mouse mAV to develop mAV NURBS-based geometric model. We then fitted the mAV geometric model to the in vivo data to reconstruct the 3D in vivo mAV geometry in the closed and open states in n = 3 mAV. Results demonstrated high fidelity geometric results. To our knowledge, this is the first time such reconstruction was ever achieved. This robust assessment of in vivo mAV leaflet kinematics in 3D opens up the possibility for longitudinal characterization of murine models that develop aortic valve disease.

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来源期刊
Annals of Biomedical Engineering
Annals of Biomedical Engineering 工程技术-工程:生物医学
CiteScore
7.50
自引率
15.80%
发文量
212
审稿时长
3 months
期刊介绍: Annals of Biomedical Engineering is an official journal of the Biomedical Engineering Society, publishing original articles in the major fields of bioengineering and biomedical engineering. The Annals is an interdisciplinary and international journal with the aim to highlight integrated approaches to the solutions of biological and biomedical problems.
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