基于Oplegnathus punctatus Cdkn1/srsf3基因间区的DNA插入特异性标记的开发,用于性别鉴定。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yuting Ma, Yongshuang Xiao, Zhizhong Xiao, Jun Li
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引用次数: 0

摘要

斑刀鱼(Oplegnathus punctatus)是一种具有较高食用和生态价值的海洋经济鱼类,其生长过程具有明显的雌雄性二型,雄鱼生长速度明显快于雌鱼。然而,目前的性别鉴定技术尚不成熟,这将限制O. punctatus的养殖生长速度和分性繁殖效率,因此开发高效的性别分子标记势在必行。本研究通过全基因组扫描、比较基因组学和结构变异分析,在雄性O. punctatus的cdkn1/srsf3基因间区发现了一个926 bp的DNA插入片段。根据雄性个体 Y 染色体基因间区的插入信息设计了一对引物。琼脂糖凝胶电泳显示,在雄性标点虫个体中扩增出两个DNA片段,分别为1118 bp和192 bp。在雄性个体中,926 bp片段被鉴定为插入到cdkn1/srsf3的基因间区,而在雌性个体中仅扩增出一个192 bp的DNA片段。通过这种方法鉴定的个体的生物学性别与其已知的表型性别一致。在这项研究中,我们开发了一种检测O. punctatus基因间区DNA插入变异的方法。此外,我们还引入了一种新的DNA标记,用于快速鉴定O. punctatus的性别,提高了检测效率。该文在性别鉴定、全雄育种、品系选择等方面具有重要的参考意义和应用价值。该文对cdkn1/srsf3基因间区的变异调控和RNA剪切的研究提供了新的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification

Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification

Development of DNA Insertion-specific Markers Based on the Intergenic Region of Oplegnathus punctatus Cdkn1/srsf3 for Sex Identification

Spotted knifejaw (Oplegnathus punctatus) is a marine economic fish with high food and ecological value, and its growth process has obvious male and female sexual dimorphism, with males growing significantly faster than females. However, the current sex identification technology is not yet mature, which will limit the growth rate of O. punctatus aquaculture and the efficiency of separate sex breeding, so the development of efficient sex molecular markers is imperative. This study identified a 926 bp DNA insertion fragment in the cdkn1/srsf3 intergenic region of O. punctatus males through whole-genome scanning, comparative genomics, and structural variant analysis. A pair of primers was designed based on the insertion information of the Y chromosome intergenic region in male individuals. Agarose gel electrophoresis revealed the amplification of two DNA fragments, 1118 bp and 192 bp, in male O. punctatus individuals. The 926 bp fragment was identified as the insertion in the intergenic region of cdkn1/srsf3 in males, while only a single 192 bp DNA fragment was amplified in females. The biological sex of the individuals identified in this manner was consistent with their known phenotypic sex. In this study, we developed a method to detect DNA insertion variants in the intergenic region of O. punctatus. Additionally, we introduced a new DNA marker for the rapid identification of the sex of O. punctatus, which enhances detection efficiency. The text has important reference significance and application value in sex identification, all-male breeding, and lineage selection. It provides new insights into the regulation of variation in the intergenic region of cdkn1/srsf3 genes and the study of RNA shearing.

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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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