从中国山东非孕期成人中分离出的无乳链球菌的基因组特征和耐药性特征

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Xinyi Gong , Yan Jin , Xiao Han , Xueqi Jiang , Beibei Miao , Shuang Meng , Jingyi Zhang , Haijian Zhou , Han Zheng , Jie Feng , Juan Li
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引用次数: 0

摘要

背景:无乳链球菌是一种公认的主要影响婴儿和孕妇的病原体。然而,由于其临床影响的严重性和多样性,它在非妊娠成人中引起侵袭性感染的作用日益重要,已成为一个重大的健康问题:方法:从中国山东的非孕期成人中分离出与临床感染相关的非重复性 S. agalactiae 临床菌株(n=139)。对这些菌株进行了抗生素敏感性测试、全基因组测序和基因组分析,以确定这些菌株的基因组特征和耐药性特征:结果:这些菌株对青霉素、氨苄西林、头孢他啶、美罗培南、利奈唑胺和万古霉素具有普遍的敏感性。值得注意的是,红霉素(91.4%)、林可霉素(89.2%)、左氧氟沙星(84.2%)、四环素(54.0%)的耐药率较高,氯霉素(12.9%)的耐药率较低。血清分型显示有 7 个血清型和 1 个不可分型菌株。血清型以 Ia、Ib、III 和 V 型为主,占菌株总数的 95.7%。19 种序列类型被分为 7 个克隆复合体,其中 CC10 最为普遍,占 48.9%。抗性基因 mreA(100%)、ermB(70.5%)和 tetM(46.0%)被普遍检测到。所有分离菌至少携带一种柔毛骨架决定簇和一种α样蛋白基因,其中以 PI-1+PI-2a 和 bca 基因最为常见,分别占 84.2% 和 54.7%:结论:虽然非妊娠期成人中的无乳链球菌菌株对β-内酰胺类抗生素仍然敏感,但对红霉素、林可霉素、左氧氟沙星和四环素的耐药性升高令人担忧。鉴于全球老年人口不断增加,感染 S. agalactiae 的负担十分沉重。持续监测血清型分布和抗生素耐药性模式对于制定有针对性的预防和治疗策略至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genomic characterization and resistance features of Streptococcus agalactiae isolated from non-pregnant adults in Shandong, China

Background

Streptococcus agalactiae is a recognized pathogen that primarily affects infants and pregnant women. However, its increasingly important role in causing invasive infections among non-pregnant adults has become a significant health concern due to the severity and variety of its clinical impacts.

Methods

Nonduplicate S. agalactiae clinical strains associated with clinical infections (n = 139) were isolated from non-pregnant adults in Shandong, China. Antibiotic susceptibility testing, whole-genome sequencing and genomic analyses were conducted to characterize the genome and identify resistance features of these strains.

Results

The strains exhibited universal susceptibility to penicillin, ampicillin, cefotaxime, meropenem, linezolid and vancomycin. Notably, high resistance rates were observed for erythromycin (91.4%), clindamycin (89.2%), levofloxacin (84.2%), tetracycline (54.0%) and, to a lesser extent, chloramphenicol (12.9%). Serotyping revealed seven serotypes and one non-typeable strain. Serotypes Ia, Ib, III and V predominated, representing 95.7% of the strains. Nineteen sequence types were categorized into seven clonal complexes, with CC10 being the most prevalent at 48.9%. The resistance genes mreA (100%), ermB (70.5%) and tetM (46.0%) were commonly detected. All the isolates carried at least one pilus backbone determinant and one alpha-like protein gene, with the PI-1+PI-2a and the bca gene being the most frequent at 84.2% and 54.7%, respectively.

Conclusions

While S. agalactiae strains in non-pregnant adults retain sensitivity to β-lactam antibiotics, the elevated resistance to erythromycin, clindamycin, levofloxacin and tetracycline is concerning. Given the growing elderly population worldwide, the burden of S. agalactiae infections is significant. Continuous surveillance of serotype distribution and antibiotic resistance patterns is imperative for targeted prevention and therapeutic strategies.

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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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