Q Wang, B Song, S Hao, Z Xiao, L Jin, T Zheng, F Chai
{"title":"[甲状腺乳头状癌中 CCND2 表达的生物信息学分析及其对免疫浸润的影响]。","authors":"Q Wang, B Song, S Hao, Z Xiao, L Jin, T Zheng, F Chai","doi":"10.12122/j.issn.1673-4254.2024.05.21","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate cyclin D2 (CCND2) expression in papillary thyroid carcinoma (PTC) and its association with the clinicopathological features.</p><p><strong>Methods: </strong>The public databases TCGA, TIMER 2.0 and UALCAN were used to explore CCND2 expression level in PTC and adjacent tissues, and its diagnostic value for PTC was analyzed using ROC curves. GO enrichment analysis of CCND2-related differentially expressed genes (DEGs) in PTC was performed, and tumor immune infiltration of CCND2 in thyroid cancer was analyzed using TIMER database and CIBERSORT data source. RT-qPCR and Western blot were used to detect CCND2 expression in normal human thyroid cell line Nthy-ori-3-1 and human PTC cell lines TPC-1 and BCPAP. CCND2 expression was also detected in clinical specimens of PTC and adjacent tissues by immunohistochemistry, and its correlation with clinicopathological features of the patients were analyzed.</p><p><strong>Results: </strong>Informatic analysis revealed significantly higher CCND2 mRNA expression in thyroid cancer than in the adjacent tissues (<i>P</i> < 0.001) in close correlation with tumor stage, gender, age, pathological subtype, and lymph node involvement (<i>P</i> < 0.05). ROC curve analysis showed that at the cutoff value of 4.983, the diagnostic sensitivity, specificity, and accuracy of CCND2 expression for PTC was 83.6%, 94.9%, and 78.5%, respectively. CCND2 expression was positively correlated with B cells, CD4<sup>+</sup> T cells, and macrophages (<i>P</i> < 0.001) and negatively with CD8<sup>+</sup> T cells (<i>P</i> < 0.01), and also correlated with memory B-cell infiltration, CD4<sup>+</sup> T-cell memory activation, M2 macrophages, resting mast cells, and mast cell activation (<i>P</i> < 0.05). RT-qPCR, Western blot and immunohistochemistry showed significantly higher CCND2 expression in the PTC cells than in Nthy-ori-3-1 cells (<i>P</i> < 0.01) and also in clinical PTC tissues than in the adjacent tissues (<i>P</i> < 0.05) in correlation with tumor size, lymph node metastasis and TNM stage (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>CCND2 overexpression is closely correlated with tumor progression and immune cell infiltration in PTC patients..</p>","PeriodicalId":18962,"journal":{"name":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11166724/pdf/","citationCount":"0","resultStr":"{\"title\":\"[Bioinformatic analysis of CCND2 expression in papillary thyroid carcinoma and its impact on immune infiltration].\",\"authors\":\"Q Wang, B Song, S Hao, Z Xiao, L Jin, T Zheng, F Chai\",\"doi\":\"10.12122/j.issn.1673-4254.2024.05.21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate cyclin D2 (CCND2) expression in papillary thyroid carcinoma (PTC) and its association with the clinicopathological features.</p><p><strong>Methods: </strong>The public databases TCGA, TIMER 2.0 and UALCAN were used to explore CCND2 expression level in PTC and adjacent tissues, and its diagnostic value for PTC was analyzed using ROC curves. GO enrichment analysis of CCND2-related differentially expressed genes (DEGs) in PTC was performed, and tumor immune infiltration of CCND2 in thyroid cancer was analyzed using TIMER database and CIBERSORT data source. RT-qPCR and Western blot were used to detect CCND2 expression in normal human thyroid cell line Nthy-ori-3-1 and human PTC cell lines TPC-1 and BCPAP. CCND2 expression was also detected in clinical specimens of PTC and adjacent tissues by immunohistochemistry, and its correlation with clinicopathological features of the patients were analyzed.</p><p><strong>Results: </strong>Informatic analysis revealed significantly higher CCND2 mRNA expression in thyroid cancer than in the adjacent tissues (<i>P</i> < 0.001) in close correlation with tumor stage, gender, age, pathological subtype, and lymph node involvement (<i>P</i> < 0.05). ROC curve analysis showed that at the cutoff value of 4.983, the diagnostic sensitivity, specificity, and accuracy of CCND2 expression for PTC was 83.6%, 94.9%, and 78.5%, respectively. CCND2 expression was positively correlated with B cells, CD4<sup>+</sup> T cells, and macrophages (<i>P</i> < 0.001) and negatively with CD8<sup>+</sup> T cells (<i>P</i> < 0.01), and also correlated with memory B-cell infiltration, CD4<sup>+</sup> T-cell memory activation, M2 macrophages, resting mast cells, and mast cell activation (<i>P</i> < 0.05). RT-qPCR, Western blot and immunohistochemistry showed significantly higher CCND2 expression in the PTC cells than in Nthy-ori-3-1 cells (<i>P</i> < 0.01) and also in clinical PTC tissues than in the adjacent tissues (<i>P</i> < 0.05) in correlation with tumor size, lymph node metastasis and TNM stage (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>CCND2 overexpression is closely correlated with tumor progression and immune cell infiltration in PTC patients..</p>\",\"PeriodicalId\":18962,\"journal\":{\"name\":\"Nan fang yi ke da xue xue bao = Journal of Southern Medical University\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-05-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11166724/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nan fang yi ke da xue xue bao = Journal of Southern Medical University\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.12122/j.issn.1673-4254.2024.05.21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12122/j.issn.1673-4254.2024.05.21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
摘要
目的研究甲状腺细胞周期蛋白D2（CCND2）在甲状腺乳头状癌（PTC）中的表达及其与临床病理特征的关系：利用公共数据库TCGA、TIMER 2.0和UALCAN探讨CCND2在PTC及邻近组织中的表达水平，并利用ROC曲线分析其对PTC的诊断价值。对PTC中CCND2相关差异表达基因（DEGs）进行了GO富集分析，并利用TIMER数据库和CIBERSORT数据源分析了CCND2在甲状腺癌中的肿瘤免疫浸润。利用 RT-qPCR 和 Western 印迹技术检测了 CCND2 在正常人甲状腺细胞系 Nthy-orii-3-1 和人 PTC 细胞系 TPC-1 和 BCPAP 中的表达。免疫组化法还检测了CCND2在PTC临床标本和邻近组织中的表达，并分析了其与患者临床病理特征的相关性：信息分析显示，CCND2 mRNA在甲状腺癌中的表达明显高于邻近组织（P＜0.001），与肿瘤分期、性别、年龄、病理亚型和淋巴结受累密切相关（P＜0.05）。ROC曲线分析表明，在临界值为4.983时，CCND2表达对PTC的诊断敏感性、特异性和准确性分别为83.6%、94.9%和78.5%。CCND2 的表达与 B 细胞、CD4+ T 细胞和巨噬细胞呈正相关（P < 0.001），与 CD8+ T 细胞呈负相关（P < 0.01），还与记忆性 B 细胞浸润、CD4+ T 细胞记忆激活、M2 巨噬细胞、静止肥大细胞和肥大细胞激活相关（P < 0.05）。RT-qPCR、Western印迹和免疫组化显示，PTC细胞中CCND2的表达明显高于Nthy-ori-3-1细胞（P＜0.01），临床PTC组织中CCND2的表达也明显高于邻近组织（P＜0.05），与肿瘤大小、淋巴结转移和TNM分期相关（P＜0.05）：结论：CCND2过表达与PTC患者的肿瘤进展和免疫细胞浸润密切相关。
[Bioinformatic analysis of CCND2 expression in papillary thyroid carcinoma and its impact on immune infiltration].
Objective: To investigate cyclin D2 (CCND2) expression in papillary thyroid carcinoma (PTC) and its association with the clinicopathological features.
Methods: The public databases TCGA, TIMER 2.0 and UALCAN were used to explore CCND2 expression level in PTC and adjacent tissues, and its diagnostic value for PTC was analyzed using ROC curves. GO enrichment analysis of CCND2-related differentially expressed genes (DEGs) in PTC was performed, and tumor immune infiltration of CCND2 in thyroid cancer was analyzed using TIMER database and CIBERSORT data source. RT-qPCR and Western blot were used to detect CCND2 expression in normal human thyroid cell line Nthy-ori-3-1 and human PTC cell lines TPC-1 and BCPAP. CCND2 expression was also detected in clinical specimens of PTC and adjacent tissues by immunohistochemistry, and its correlation with clinicopathological features of the patients were analyzed.
Results: Informatic analysis revealed significantly higher CCND2 mRNA expression in thyroid cancer than in the adjacent tissues (P < 0.001) in close correlation with tumor stage, gender, age, pathological subtype, and lymph node involvement (P < 0.05). ROC curve analysis showed that at the cutoff value of 4.983, the diagnostic sensitivity, specificity, and accuracy of CCND2 expression for PTC was 83.6%, 94.9%, and 78.5%, respectively. CCND2 expression was positively correlated with B cells, CD4+ T cells, and macrophages (P < 0.001) and negatively with CD8+ T cells (P < 0.01), and also correlated with memory B-cell infiltration, CD4+ T-cell memory activation, M2 macrophages, resting mast cells, and mast cell activation (P < 0.05). RT-qPCR, Western blot and immunohistochemistry showed significantly higher CCND2 expression in the PTC cells than in Nthy-ori-3-1 cells (P < 0.01) and also in clinical PTC tissues than in the adjacent tissues (P < 0.05) in correlation with tumor size, lymph node metastasis and TNM stage (P < 0.05).
Conclusion: CCND2 overexpression is closely correlated with tumor progression and immune cell infiltration in PTC patients..
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