在家兔水体中同时测定布林佐胺降解产物(主要代谢物)中的溴莫尼丁和布林佐胺的简便有效的液相色谱-紫外法。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Marwa A Wahab Mubarak, M Abdul-Azim Mohammad, Ramzia I El-Bagary, Ehab Elkady, Nisreen F Abo-Talib
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引用次数: 0

摘要

建立并验证了一种灵敏、特异、可靠和低成本的LC-UV方法,用于同时定量检测兔水房水(AH)中酒石酸溴莫尼定(BM)和布林佐胺(BZ)的含量,N-去乙基布林佐胺(NDBZ)是BZ的主要降解产物,也被认为是其主要代谢产物。盐酸多佐胺(DZ)用作内标(IS)。使用 ZnSO4.7H2O 作为脱蛋白剂,通过简单的预处理从兔 AH 样品中提取分析物。分析物在氰丙沃特斯色谱柱(4.6 × 200 mm, 5 μm)上分离,流动相为 25 mM 乙酸铵 pH 4.5(用 85% 磷酸调节):甲醇:乙腈(94:4.5:1.5, v/v),流速为 1.0 mL min-1。检测波长为 254 纳米。兔 AH 中的定量下限为 100.0 纳克 mL-1。根据 EMA 指南对该方法进行了验证。结果表明,该方法在 BM 和 BZ 的 100.0-1000.0 ng mL-1 范围内准确、精确、线性。该方法简便、安全、环保、快速、准确,可用于BM和BZ的定量分析,并能成功分离出被认为是BZ潜在刺激性降解产物的NDBZ。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Simple Validated LC-UV Method for the Simultaneous Determination of Brimonidine and Brinzolamide in the Presence of Brinzolamide's Degradation Product (Major Metabolite) in Rabbit Aqueous Humor.

A sensitive, specific, reliable and low-cost LC-UV method has been developed and validated for simultaneous quantification of brimonidine tartrate (BM) and brinzolamide (BZ) in rabbit aqueous humor (AH) in the presence of N-desethyl-brinzolamide (NDBZ); BZ is a major degradation product, and it is also considered to be its major metabolite. Dorzolamide hydrochloride (DZ) was used as an internal standard (IS). The analytes were extracted from rabbit AH samples by a simple pre-treatment utilizing ZnSO4.7H2O as a deproteinizing agent. The analytes were separated on a cyanopropyl Waters column (4.6 × 200 mm, 5 μm) with an isocratic mobile phase consisting of 25 mM ammonium acetate pH 4.5 (adjusted with 85% phosphoric acid):methanol:acetonitrile (94:4.5:1.5, v/v) at a flow rate of 1.0 mL min-1. The detection was done at 254 nm. The lower limit of quantification in rabbit AH was 100.0 ng mL-1. The method was validated according to EMA guidelines. The method was confirmed to be accurate, precise and linear over a range of 100.0-1000.0 ng mL-1 for BM and BZ. The method developed herein is simple, safe, eco-friendly, rapid and accurately applied for the quantification of BM and BZ, along with the successful separation of NDBZ, which is considered as a potential irritant degradation product of BZ.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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