Munisamy Prathaban, Ragothaman Prathiviraj, Mythili Ravichandran, Sharmila Devi Natarajan, Murugesan Sobanaa, S Hari Krishna Kumar, Varadharaju Chandrasekar, Joseph Selvin
{"title":"Isoptericola haloaureus sp.","authors":"Munisamy Prathaban, Ragothaman Prathiviraj, Mythili Ravichandran, Sharmila Devi Natarajan, Murugesan Sobanaa, S Hari Krishna Kumar, Varadharaju Chandrasekar, Joseph Selvin","doi":"10.1007/s10482-024-01985-7","DOIUrl":null,"url":null,"abstract":"<p><p>Strain MP-1014<sup>T</sup>, an obligate halophilic actinobacterium, was isolated from the mangrove soil of Thandavarayancholanganpettai, Tamil Nadu, India. A polyphasic approach was utilized to explore its phylogenetic position completely. The isolate was Gram-positive, filamentous, non-motile, and coccoid in older cultures. Ideal growth conditions were seen at 30 °C and pH 7.0, with 5% NaCl (W/V), and the DNA G + C content was 73.3%. The phylogenic analysis of this strain based upon 16S rRNA gene sequence revealed 97-99.8% similarity to the recognized species of the genus Isoptericola. Strain MP-1014<sup>T</sup> exhibits the highest similarity to I. sediminis JC619<sup>T</sup> (99.7%), I. chiayiensis KCTC19740<sup>T</sup> (98.9%), and subsequently to I. halotolerans KCTC19646<sup>T</sup> (98.6%), when compared with other members within the Isoptericola genus (< 98%). ANI scores of strain MP-1014<sup>T</sup> are 86.4%, 84.2%, and 81.5% and dDDH values are 59.7%, 53.6%, and 34.8% with I. sediminis JC619<sup>T</sup>, I. chiayiensis KCTC19740<sup>T</sup> and I. halotolerans KCTC19646<sup>T</sup> respectively. The major polar lipids of the strain MP-1014<sup>T</sup> were phosphatidylinositol, phosphatidylglycerol, diphosphotidylglycerol, two unknown phospholipids, and glycolipids. The predominant respiratory menaquinones were MK<sub>9</sub> (H<sub>4</sub>) and MK<sub>9</sub> (H<sub>2</sub>). The major fatty acids were anteiso-C<sub>15:0</sub>, anteiso-C<sub>17:0</sub>, iso-C<sub>14:0</sub>, C<sub>15:0,</sub> and C<sub>16:0</sub>. Also, initial genome analysis of the organism suggests it as a biostimulant for enhancing agriculture in saline environments. Based on phenotypic and genetic distinctiveness, the strain MP-1014 <sup>T</sup> represents the novel species of the genus Isoptericola assigned Isoptericola haloaureus sp. nov., is addressed by the strain MP-1014 <sup>T</sup>, given its phenotypic, phylogenetic, and hereditary uniqueness. The type strain is MP-1014<sup>T</sup> [(NCBI = OP672482.1 = GCA_036689775.1) ATCC = BAA 2646<sup>T</sup>; DSMZ = 29325<sup>T</sup>; MTCC = 13246<sup>T</sup>].</p>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Isoptericola haloaureus sp. nov., a dimorphic actinobacterium isolated from mangrove sediments of southeast India, implicating biosaline agricultural significance through nitrogen fixation and salt tolerance genes.\",\"authors\":\"Munisamy Prathaban, Ragothaman Prathiviraj, Mythili Ravichandran, Sharmila Devi Natarajan, Murugesan Sobanaa, S Hari Krishna Kumar, Varadharaju Chandrasekar, Joseph Selvin\",\"doi\":\"10.1007/s10482-024-01985-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Strain MP-1014<sup>T</sup>, an obligate halophilic actinobacterium, was isolated from the mangrove soil of Thandavarayancholanganpettai, Tamil Nadu, India. A polyphasic approach was utilized to explore its phylogenetic position completely. The isolate was Gram-positive, filamentous, non-motile, and coccoid in older cultures. Ideal growth conditions were seen at 30 °C and pH 7.0, with 5% NaCl (W/V), and the DNA G + C content was 73.3%. The phylogenic analysis of this strain based upon 16S rRNA gene sequence revealed 97-99.8% similarity to the recognized species of the genus Isoptericola. Strain MP-1014<sup>T</sup> exhibits the highest similarity to I. sediminis JC619<sup>T</sup> (99.7%), I. chiayiensis KCTC19740<sup>T</sup> (98.9%), and subsequently to I. halotolerans KCTC19646<sup>T</sup> (98.6%), when compared with other members within the Isoptericola genus (< 98%). ANI scores of strain MP-1014<sup>T</sup> are 86.4%, 84.2%, and 81.5% and dDDH values are 59.7%, 53.6%, and 34.8% with I. sediminis JC619<sup>T</sup>, I. chiayiensis KCTC19740<sup>T</sup> and I. halotolerans KCTC19646<sup>T</sup> respectively. The major polar lipids of the strain MP-1014<sup>T</sup> were phosphatidylinositol, phosphatidylglycerol, diphosphotidylglycerol, two unknown phospholipids, and glycolipids. The predominant respiratory menaquinones were MK<sub>9</sub> (H<sub>4</sub>) and MK<sub>9</sub> (H<sub>2</sub>). The major fatty acids were anteiso-C<sub>15:0</sub>, anteiso-C<sub>17:0</sub>, iso-C<sub>14:0</sub>, C<sub>15:0,</sub> and C<sub>16:0</sub>. Also, initial genome analysis of the organism suggests it as a biostimulant for enhancing agriculture in saline environments. Based on phenotypic and genetic distinctiveness, the strain MP-1014 <sup>T</sup> represents the novel species of the genus Isoptericola assigned Isoptericola haloaureus sp. nov., is addressed by the strain MP-1014 <sup>T</sup>, given its phenotypic, phylogenetic, and hereditary uniqueness. The type strain is MP-1014<sup>T</sup> [(NCBI = OP672482.1 = GCA_036689775.1) ATCC = BAA 2646<sup>T</sup>; DSMZ = 29325<sup>T</sup>; MTCC = 13246<sup>T</sup>].</p>\",\"PeriodicalId\":50746,\"journal\":{\"name\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2024-06-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10482-024-01985-7\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10482-024-01985-7","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Isoptericola haloaureus sp. nov., a dimorphic actinobacterium isolated from mangrove sediments of southeast India, implicating biosaline agricultural significance through nitrogen fixation and salt tolerance genes.
Strain MP-1014T, an obligate halophilic actinobacterium, was isolated from the mangrove soil of Thandavarayancholanganpettai, Tamil Nadu, India. A polyphasic approach was utilized to explore its phylogenetic position completely. The isolate was Gram-positive, filamentous, non-motile, and coccoid in older cultures. Ideal growth conditions were seen at 30 °C and pH 7.0, with 5% NaCl (W/V), and the DNA G + C content was 73.3%. The phylogenic analysis of this strain based upon 16S rRNA gene sequence revealed 97-99.8% similarity to the recognized species of the genus Isoptericola. Strain MP-1014T exhibits the highest similarity to I. sediminis JC619T (99.7%), I. chiayiensis KCTC19740T (98.9%), and subsequently to I. halotolerans KCTC19646T (98.6%), when compared with other members within the Isoptericola genus (< 98%). ANI scores of strain MP-1014T are 86.4%, 84.2%, and 81.5% and dDDH values are 59.7%, 53.6%, and 34.8% with I. sediminis JC619T, I. chiayiensis KCTC19740T and I. halotolerans KCTC19646T respectively. The major polar lipids of the strain MP-1014T were phosphatidylinositol, phosphatidylglycerol, diphosphotidylglycerol, two unknown phospholipids, and glycolipids. The predominant respiratory menaquinones were MK9 (H4) and MK9 (H2). The major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C14:0, C15:0, and C16:0. Also, initial genome analysis of the organism suggests it as a biostimulant for enhancing agriculture in saline environments. Based on phenotypic and genetic distinctiveness, the strain MP-1014 T represents the novel species of the genus Isoptericola assigned Isoptericola haloaureus sp. nov., is addressed by the strain MP-1014 T, given its phenotypic, phylogenetic, and hereditary uniqueness. The type strain is MP-1014T [(NCBI = OP672482.1 = GCA_036689775.1) ATCC = BAA 2646T; DSMZ = 29325T; MTCC = 13246T].
期刊介绍：
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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