Isoptericola haloaureus sp.

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Munisamy Prathaban, Ragothaman Prathiviraj, Mythili Ravichandran, Sharmila Devi Natarajan, Murugesan Sobanaa, S. Hari Krishna Kumar, Varadharaju Chandrasekar, Joseph Selvin
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引用次数: 0

摘要

从印度泰米尔纳德邦 Thandavarayancholanganpettai 的红树林土壤中分离出了 MP-1014T 菌株,这是一种嗜卤放线菌。采用多相法对其系统发育位置进行了全面探索。该分离菌呈革兰氏阳性、丝状、非运动性,在老培养物中呈茧状。理想的生长条件是温度为 30 °C,pH 值为 7.0,NaCl 含量为 5%(W/V),DNA G + C 含量为 73.3%。根据 16S rRNA 基因序列对该菌株进行的系统发育分析表明,它与公认的 Isoptericola 属物种的相似度为 97-99.8%。菌株 MP-1014T 与 I. sediminis JC619T(99.7%)、I. chiayiensis KCTC19740T(98.9%)和 I. halotolerans KCTC19646T(98.6%),与 Isoptericola 属中的其他成员相比(I. sediminis JC619T、I. chiayiensis KCTC19740T 和 I. halotolerans KCTC19646T 的 T 值分别为 86.4%、84.2% 和 81.5%,dDDH 值分别为 59.7%、53.6% 和 34.8%)。MP-1014T 菌株的主要极性脂质为磷脂酰肌醇、磷脂酰甘油、二磷酰甘油、两种未知磷脂和糖脂。主要的呼吸型甲萘醌是 MK9 (H4) 和 MK9 (H2)。主要脂肪酸为前-C15:0、前-C17:0、异-C14:0、C15:0 和 C16:0。此外,对该生物体的初步基因组分析表明,它是一种生物刺激剂,可用于改善盐碱环境中的农业。鉴于其表型、系统发育和遗传的独特性,MP-1014 T 菌株代表了 Isoptericola 属的新物种,被分配为 Isoptericola haloaureus sp.模式菌株为 MP-1014T[(NCBI = OP672482.1 = GCA_036689775.1)ATCC = BAA 2646T;DSMZ = 29325T;MTCC = 13246T]。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Isoptericola haloaureus sp. nov., a dimorphic actinobacterium isolated from mangrove sediments of southeast India, implicating biosaline agricultural significance through nitrogen fixation and salt tolerance genes

Isoptericola haloaureus sp. nov., a dimorphic actinobacterium isolated from mangrove sediments of southeast India, implicating biosaline agricultural significance through nitrogen fixation and salt tolerance genes

Strain MP-1014T, an obligate halophilic actinobacterium, was isolated from the mangrove soil of Thandavarayancholanganpettai, Tamil Nadu, India. A polyphasic approach was utilized to explore its phylogenetic position completely. The isolate was Gram-positive, filamentous, non-motile, and coccoid in older cultures. Ideal growth conditions were seen at 30 °C and pH 7.0, with 5% NaCl (W/V), and the DNA G + C content was 73.3%. The phylogenic analysis of this strain based upon 16S rRNA gene sequence revealed 97–99.8% similarity to the recognized species of the genus Isoptericola. Strain MP-1014T exhibits the highest similarity to I. sediminis JC619T (99.7%), I. chiayiensis KCTC19740T (98.9%), and subsequently to I. halotolerans KCTC19646T (98.6%), when compared with other members within the Isoptericola genus (< 98%). ANI scores of strain MP-1014T are 86.4%, 84.2%, and 81.5% and dDDH values are 59.7%, 53.6%, and 34.8% with I. sediminis JC619T, I. chiayiensis KCTC19740T and I. halotolerans KCTC19646T respectively. The major polar lipids of the strain MP-1014T were phosphatidylinositol, phosphatidylglycerol, diphosphotidylglycerol, two unknown phospholipids, and glycolipids. The predominant respiratory menaquinones were MK9 (H4) and MK9 (H2). The major fatty acids were anteiso-C15:0, anteiso-C17:0, iso-C14:0, C15:0, and C16:0. Also, initial genome analysis of the organism suggests it as a biostimulant for enhancing agriculture in saline environments. Based on phenotypic and genetic distinctiveness, the strain MP-1014 T represents the novel species of the genus Isoptericola assigned Isoptericola haloaureus sp. nov., is addressed by the strain MP-1014 T, given its phenotypic, phylogenetic, and hereditary uniqueness. The type strain is MP-1014T [(NCBI = OP672482.1 = GCA_036689775.1) ATCC = BAA 2646T; DSMZ = 29325T; MTCC = 13246T].

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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