重复经颅磁刺激(rTMS)对缺血性中风成年雄性小鼠神经重塑和运动功能的影响。

IF 2.9 3区 医学 Q2 NEUROSCIENCES
Wang Peipei, Deng Yu, Lin Xiaoyan, Liu Yunxia, Liang Liuming, Cheng Tongbin, Lv Shaoping
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引用次数: 0

摘要

小胶质细胞过度激活引起的神经炎症在缺血性中风的发病机制中起着关键作用。重复经颅磁刺激(rTMS)是一种非侵入性神经调节技术,最近有报道称它能调节小胶质细胞功能并发挥抗炎作用。经颅磁刺激中的间歇性脉冲刺激(iTBS)方案可提高神经元的兴奋性。然而,iTBS 是否通过刺激神经元从而调节小胶质细胞的极化来发挥抗炎作用仍不清楚。我们对大脑中动脉闭塞/再灌注(MCAO/r)损伤的成年雄性小鼠进行了为期一周的经颅磁刺激(iTBS 方案)治疗后的运动功能评估。我们还利用细胞增殖试验、多重细胞因子生物测定和免疫荧光染色研究了与小胶质细胞极化相关的分子生物学改变。 iTBS疗法能改善早期脑缺血损伤小鼠的平衡和运动协调功能,增加自发运动,改善行走功能。MCAO损伤小鼠体内IL-1β、TNF-α和IL-10的表达水平显著增加。尤其是经颅磁刺激能明显增加梗死皮质中增殖细胞的数量。MCAO损伤小鼠梗死周围区域的MAP2荧光强度较低,但信号范围较广。经颅磁刺激可抑制促炎性M1活化(Iba1+/CD86+),改善梗死周围区抗炎性M2活化(Iba1+/CD206+),从而显著改变M1/M2的表型比例。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of a novel regimen of repetitive transcranial magnetic stimulation (rTMS) on neural remodeling and motor function in adult male mice with ischemic stroke

Effects of a novel regimen of repetitive transcranial magnetic stimulation (rTMS) on neural remodeling and motor function in adult male mice with ischemic stroke

Neuroinflammation caused by excessive microglial activation plays a key role in the pathogenesis of ischemic stroke. Repetitive transcranial magnetic stimulation (rTMS) is a noninvasive neuromodulatory technique that has recently been reported to regulate microglial functions and exert anti-inflammatory effects. The intermittent burst stimulation (iTBS) regimen in rTMS improves neuronal excitability. However, whether iTBS exerts its anti-inflammatory effects by stimulating neurons and thereby modulating microglial polarization remains unclear. Motor function was assessed after 1 week of rTMS (iTBS regimen) treatment in adult male mice with occlusion/reperfusion of the middle cerebral artery (MCAO/r) injury. We also investigated the molecular biological alterations associated with microglial polarization using a cell proliferation assay, multiplex cytokine bioassays, and immunofluorescence staining. iTBS regimen can improve balance and motor coordination function, increase spontaneous movement, and improve walking function in mice with early cerebral ischemia injury. Expression levels of IL-1β, TNF-α, and IL-10 increased significantly in mice with MCAO injury. Especially, rTMS significantly increased the number of proliferating cells in the infarcted cortex. The fluorescence intensity of MAP2 in the peri-infarct area of MCAO injured mice was low, but the signal was broader. Compared with MCAO group, the fluorescence intensity of MAP2 in rTMS group was significantly increased. rTMS inhibited pro-inflammatory M1 activation (Iba1+/CD86+) and improved anti-inflammatory M2 activation (Iba1+/CD206+) in the peri-infarct zone, thus significantly changing the phenotypic ratio M1/M2. rTMS improves motor dysfunction and neuroinflammation after cerebral I/R injury in mice by regulating microglial polarization.

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来源期刊
Journal of Neuroscience Research
Journal of Neuroscience Research 医学-神经科学
CiteScore
9.50
自引率
2.40%
发文量
145
审稿时长
1 months
期刊介绍: The Journal of Neuroscience Research (JNR) publishes novel research results that will advance our understanding of the development, function and pathophysiology of the nervous system, using molecular, cellular, systems, and translational approaches. JNR covers both basic research and clinical aspects of neurology, neuropathology, psychiatry or psychology. The journal focuses on uncovering the intricacies of brain structure and function. Research published in JNR covers all species from invertebrates to humans, and the reports inform the readers about the function and organization of the nervous system, with emphasis on how disease modifies the function and organization.
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